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    CHEMICAL SENSOR WITH CONDUCTIVE CUP-SHAPED SENSOR SURFACE
    61.
    发明申请
    CHEMICAL SENSOR WITH CONDUCTIVE CUP-SHAPED SENSOR SURFACE 审中-公开
    具有导电杯形传感器表面的化学传感器

    公开(公告)号：WO2013109877A3

    公开(公告)日：2013-10-03

    申请号：PCT/US2013022129

    申请日：2013-01-18

    Applicant: LIFE TECHNOLOGIES CORP ,  LI SHIFENG ,  BUSTILLO JAMES ,  HINZ WOLFGANG

    Inventor： LI SHIFENG ,  BUSTILLO JAMES ,  HINZ WOLFGANG

    IPC: G01N27/414

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/414

    Abstract: A chemical detection device and a method of its manufacture is described herein. The device includes a chemically-sensitive field effect transistor 214 including a floating gate conductor 218 having an upper surface 220. A dielectric material 210 defines a cavity 201 extending to the upper surface of the floating gate conductor. A conductive layer 250 is on a sidewall 260 of the cavity and electrically communicating with the floating gate conductor 220. An inner surface 251 of the conductive layer 250 defines a well 201 for the sensor.

    Abstract translation: 本文描述了化学检测装置及其制造方法。 该器件包括化学敏感的场效应晶体管214，其包括具有上表面220的浮栅导体218.介电材料210限定延伸到浮栅导体的上表面的空腔201。 导电层250在空腔的侧壁260上并与浮动栅极导体220电连通。导电层250的内表面251限定用于传感器的阱201。

    SYSTEMS AND METHODS FOR HYBRID ASSEMBLY OF NUCLEIC ACID SEQUENCES
    62.
    发明申请
    SYSTEMS AND METHODS FOR HYBRID ASSEMBLY OF NUCLEIC ACID SEQUENCES 审中-公开
    核酸序列杂交组合的系统和方法

    公开(公告)号：WO2012177774A3

    公开(公告)日：2013-07-18

    申请号：PCT/US2012043365

    申请日：2012-06-20

    Applicant: LIFE TECHNOLOGIES CORP ,  JIANG HONGSHAN ,  XU ZHAO ,  INGMAN MAX

    Inventor： JIANG HONGSHAN ,  XU ZHAO ,  INGMAN MAX

    IPC: G06F19/22

    CPC classification number: G06F19/22

    Abstract: Systems and methods for assembling a nucleic acid sequence are disclosed. A plurality of single fragment sequence reads and a plurality of paired fragment sequence reads are received. Each paired fragment sequence read comprises at least two sequence reads separated by an insert. Single fragment sequence reads are assembled into a plurality of contigs, and the paired fragment sequence reads are mapped to the contigs. Further, gap regions comprising a portion of the partially assembled nucleic acid sequence for which the single fragment sequence reads do not map are identified, and hanging pairwise sequence reads of the mapped paired fragment sequence reads are used to fill in the gap region.

    Abstract translation: 公开了用于组装核酸序列的系统和方法。 接收多个单片段序列读取和多个配对片段序列读取。 读取的每个配对片段序列包含由插入片段分开的至少两个序列读取。 单片段序列读取被组装成多个重叠群，并且将配对的片段序列读取映射到重叠群。 此外，识别包含单片段序列读取的部分组装的核酸序列的一部分不映射的间隙区域，并且使用映射的配对片段序列读取的悬挂成对序列读取来填充间隙区域。

    SYSTEMS AND METHODS FOR LABORATORY ASSAY VALIDATION OR VERIFICATION
    65.
    发明申请
    SYSTEMS AND METHODS FOR LABORATORY ASSAY VALIDATION OR VERIFICATION 审中-公开
    用于实验室检测或验证的系统和方法

    公开(公告)号：WO2012065092A3

    公开(公告)日：2012-11-15

    申请号：PCT/US2011060416

    申请日：2011-11-11

    Applicant: LIFE TECHNOLOGIES CORP ,  BOONYARATANAKORNKIT JERRY ,  SCHOENBRUNNER ERHARD ,  BALCER MARC

    Inventor： BOONYARATANAKORNKIT JERRY ,  SCHOENBRUNNER ERHARD ,  BALCER MARC

    IPC: G06F7/00

    CPC classification number: H04L67/42 ,  G06Q10/0639 ,  H04L29/06

    Abstract: Systems and methods are used to generate a protocol for an assay. At least one performance characteristic parameter of an assay and at least one standardized protocol for each assay of a plurality of assays and assay types are stored. A performance characteristic parameter selection and an assay selection are received from a client device of a laboratory. One or more performance characteristic parameters and a standardized protocol are retrieved from the database device. The client device is sent the one or more performance characteristic parameters and one or more study variable values. One or more amendments to the one or more performance characteristic parameters and one or more study variable values are received from the client device. A protocol for the assay is generated based on the one or more amendments.

    Abstract translation: 系统和方法用于生成测定方案。 存储测定的至少一个性能特征参数和用于多个测定和测定类型的每个测定的至少一个标准化方案。 从实验室的客户端装置接收性能特征参数选择和测定选择。 从数据库设备检索一个或多个性能特征参数和标准化协议。 客户端设备被发送一个或多个性能特征参数和一个或多个研究变量值。 从客户端设备接收对一个或多个性能特性参数和一个或多个研究变量值的一个或多个修改。 基于一个或多个修正产生测定方案。

    METHODS, COMPOSITIONS, AND KITS FOR DETECTING RARE CELLS
    67.
    发明申请
    METHODS, COMPOSITIONS, AND KITS FOR DETECTING RARE CELLS 审中-公开
    用于检测稀有细胞的方法，组合物和试剂盒

    公开(公告)号：WO2012097353A1

    公开(公告)日：2012-07-19

    申请号：PCT/US2012021397

    申请日：2012-01-14

    Applicant: LIFE TECHNOLOGIES CORP ,  DENG DAVID XINGFEI ,  CHEN CAIFU

    Inventor： DENG DAVID XINGFEI ,  CHEN CAIFU

    IPC: C12Q1/68 ,  G01N33/50

    CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/156

    Abstract: Disclosed herein are methods for identifying rare cells containing particular markers and/or alleles from biological samples that have not been substantially pre-processed (e.g., unprocessed whole blood). The methods described herein provide a system for digital enrichment of target cells from a biological sample and detection of such target cells, thereby allowing accurate and efficient detection and / or enumeration of such cells in the sample.

    Abstract translation: 本文公开了用于从未经过基本上预处理的生物样品（例如未加工的全血）鉴定含有特定标记物和/或等位基因的稀有细胞的方法。 本文描述的方法提供了用于从生物样品数字富集靶细胞并检测这样的靶细胞的系统，从而允许样品中这些细胞的准确和有效的检测和/或计数。

    SYSTEMS AND METHODS FOR THE ANALYSIS OF PROXIMITY BINDING ASSAY DATA
    68.
    发明申请
    SYSTEMS AND METHODS FOR THE ANALYSIS OF PROXIMITY BINDING ASSAY DATA 审中-公开
    用于分析接近结合测定数据的系统和方法

    公开(公告)号：WO2012068276A3

    公开(公告)日：2012-07-19

    申请号：PCT/US2011061034

    申请日：2011-11-16

    Applicant: LIFE TECHNOLOGIES CORP ,  LEONG HARRISON ,  MAJUMDAR NIVEDITA ,  SWARTZMAN ELANA

    Inventor： LEONG HARRISON ,  MAJUMDAR NIVEDITA ,  SWARTZMAN ELANA

    IPC: G06F19/24

    CPC classification number: G06F19/18 ,  G06F19/24

    Abstract: A proximity binding assay (PBA) is performed on at least one test sample, at least one reference sample, a background sample, and one or more calibration samples using a thermal cycler instrument. Ct values are determined for at least one set of test sample data and at least one set of reference sample data. Background corrected Ct values are calculated using a corresponding value in a background sample data set. A linear range is determined for the background corrected Ct values as a function of sample quantity. A linear regression line is calculated for each linear range. One or more parameter values of an exponential model (EM) fold change formula are estimated from the one or more sets of calibration sample data. A target protein quantity and associated confidence interval are calculated using the linear regression lines and the EM fold change formula.

    Abstract translation: 使用热循环仪器在至少一个测试样品，至少一个参考样品，背景样品和一个或多个校准样品上进行接近结合测定（PBA）。 对至少一组测试样本数据和至少一组参考样本数据确定Ct值。 背景校正的Ct值是使用背景样本数据集中的对应值计算的。 根据样品数量确定背景校正Ct值的线性范围。 针对每个线性范围计算线性回归线。 根据一组或多组校准样本数据估计指数模型（EM）倍数变化公式的一个或多个参数值。 使用线性回归线和EM倍数变化公式计算目标蛋白质量和相关置信区间。

    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS
    69.
    发明申请
    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 审中-公开
    序列合成方法中的替代核酸流

    公开(公告)号：WO2011156707A3

    公开(公告)日：2012-03-29

    申请号：PCT/US2011039973

    申请日：2011-06-10

    Applicant: LIFE TECHNOLOGIES CORP ,  SCHULTZ JONATHAN ,  DAVIDSON JOHN F

    Inventor： SCHULTZ JONATHAN ,  DAVIDSON JOHN F

    IPC: C12Q1/68 ,  G01N33/48 ,  G01N33/50

    CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

    Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

    ANALYTE DETECTION USING PROXIMITY PROBES
    70.
    发明申请
    ANALYTE DETECTION USING PROXIMITY PROBES 审中-公开
    使用临近探针分析检测

    公开(公告)号：WO2012006556A3

    公开(公告)日：2012-03-22

    申请号：PCT/US2011043406

    申请日：2011-07-08

    Applicant: LIFE TECHNOLOGIES CORP ,  SCHWEITZER BARRY ,  BRANCHAUD BRUCE

    Inventor： SCHWEITZER BARRY ,  BRANCHAUD BRUCE

    IPC: C12Q1/68 ,  A61K38/00 ,  G01N33/53 ,  G01N33/566

    CPC classification number: C12Q1/6816 ,  C12Q2563/179 ,  C12Q2565/101

    Abstract: Disclosed are methods of detecting target analytes using covalent analyte binding moieties in proximity detection assays. Specifically, two proximity detection probes, each comprises an analyte binding moiety and an oligonucleotide moiety, are used for the detection with one analyte binding moiety that covalently binds to the targeted analytes. Further disclosed are methods of coupling an analyte binding moiety with an oligonucleotide moiety to form a proximity detection probe during the detection assays. Reagents and kits for carrying out the methods are also provided.

    Abstract translation: 公开了使用共价分析物结合部分在接近检测测定中检测目标分析物的方法。 具体地，每个包含分析物结合部分和寡核苷酸部分的两个接近检测探针用于通过与目标分析物共价结合的一个分析物结合部分进行检测。 进一步公开的是在检测测定期间将分析物结合部分与寡核苷酸部分偶联以形成接近检测探针的方法。 还提供了用于执行方法的试剂和试剂盒。

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