公开(公告)号：EP2805157B1

公开(公告)日：2017-07-12

申请号：EP13704843.5

申请日：2013-01-18

Applicant: Life Technologies Corporation ,  Li, Shifeng ,  Bustillo, James ,  Hinz, Wolfgang

Inventor： LI, Shifeng ,  BUSTILLO, James ,  HINZ, Wolfgang

IPC: G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/414 ,  Y10T436/143333

Abstract: A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. The well wall structure defines an upper surface and defines a wall surface extending between the upper surface and the lower surface. The system further includes a conductive layer disposed over the lower surface and the wall surface.

Abstract translation: 一种系统包括传感器，该传感器包括传感器垫和限定可操作地耦合到传感器垫的井的井壁结构。 井由设置在传感器垫上的下表面进一步限定。 井壁结构限定上表面并限定在上表面和下表面之间延伸的壁表面。 该系统还包括设置在下表面和壁表面上的导电层。

公开(公告)号：EP2805157A2

公开(公告)日：2014-11-26

申请号：EP13704843.5

申请日：2013-01-18

Applicant: Life Technologies Corporation ,  Li, Shifeng ,  Bustillo, James ,  Hinz, Wolfgang

Inventor： LI, Shifeng ,  BUSTILLO, James ,  HINZ, Wolfgang

IPC: G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/414 ,  Y10T436/143333

Abstract: A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. The well wall structure defines an upper surface and defines a wall surface extending between the upper surface and the lower surface. The system further includes a conductive layer disposed over the lower surface and the wall surface.

Abstract translation: 一种系统包括传感器，其包括传感器垫和井壁结构，该结构限定了可操作地耦合到传感器垫的井。 该阱还由设置在传感器垫上的下表面限定。 井壁结构限定了上表面并限定了在上表面和下表面之间延伸的壁表面。 该系统还包括设置在下表面和壁表面上的导电层。

公开(公告)号：WO2013109877A3

公开(公告)日：2013-10-03

申请号：PCT/US2013022129

申请日：2013-01-18

Applicant: LIFE TECHNOLOGIES CORP ,  LI SHIFENG ,  BUSTILLO JAMES ,  HINZ WOLFGANG

Inventor： LI SHIFENG ,  BUSTILLO JAMES ,  HINZ WOLFGANG

IPC: G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/414

Abstract: A chemical detection device and a method of its manufacture is described herein. The device includes a chemically-sensitive field effect transistor 214 including a floating gate conductor 218 having an upper surface 220. A dielectric material 210 defines a cavity 201 extending to the upper surface of the floating gate conductor. A conductive layer 250 is on a sidewall 260 of the cavity and electrically communicating with the floating gate conductor 220. An inner surface 251 of the conductive layer 250 defines a well 201 for the sensor.

Abstract translation: 本文描述了化学检测装置及其制造方法。 该器件包括化学敏感的场效应晶体管214，其包括具有上表面220的浮栅导体218.介电材料210限定延伸到浮栅导体的上表面的空腔201。 导电层250在空腔的侧壁260上并与浮动栅极导体220电连通。导电层250的内表面251限定用于传感器的阱201。

公开(公告)号：WO2012031234A3

公开(公告)日：2012-03-08

申请号：PCT/US2011/050389

申请日：2011-09-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LI, Shifeng ,  LAFFERTY, Michael, W. ,  KOTSEROGLOU, Theo ,  OLDHAM, Mark

Inventor： LI, Shifeng ,  LAFFERTY, Michael, W. ,  KOTSEROGLOU, Theo ,  OLDHAM, Mark

IPC: B01J19/00 ,  B01L3/00 ,  G01N21/64

Abstract: Sequencing of nucleic acid strands, particularly DNA,has became increasingly important. The sequence of a nucleic acid sample can be determined by measuring ionic responses to nucleic acid sample can be determined by measuring fluorescent emissions resulting from nucleotide addition. The sequencing device includes a substrate having a refractive index of at least 1.45 and a surface layer disposed over a major surface of the substrate and defining a plurality of reaction volumes. The surface layer has a refractive index in a range of 1.32 to 1.38. The substrate may also comprise a pluralityof detectors.

公开(公告)号：WO2013109877A2

公开(公告)日：2013-07-25

申请号：PCT/US2013/022129

申请日：2013-01-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LI, Shifeng ,  BUSTILLO, James ,  HINZ, Wolfgang

Inventor： LI, Shifeng ,  BUSTILLO, James ,  HINZ, Wolfgang

IPC: G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/414 ,  Y10T436/143333

Abstract: In one implementation, a chemical detection device is described herein. The device includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines a cavity extending to the upper surface of the floating gate conductor. A conductive layer is on a sidewall of the cavity and electrically communicating with the floating gate conductor. An inner surface of the conductive layer defines a well for the sensor.

Abstract translation: 在一个实施方案中，本文描述了化学检测装置。 该器件包括具有上表面的浮栅导体的化学敏感场效应晶体管。 介电材料限定延伸到浮动栅极导体的上表面的空腔。 导电层位于空腔的侧壁上并与浮动栅极导体电连通。 导电层的内表面限定了传感器的孔。

公开(公告)号：WO2012024500A1

公开(公告)日：2012-02-23

申请号：PCT/US2011/048268

申请日：2011-08-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  HINZ, Wolfgang ,  MAURO, John, Matthew ,  LI, Shifeng ,  BUSTILLO, James, M.

Inventor： HINZ, Wolfgang ,  MAURO, John, Matthew ,  LI, Shifeng ,  BUSTILLO, James, M.

IPC: G01N27/26

CPC classification number: G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls, The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

Abstract translation: 所描述的实施例可以提供一种制造化学检测装置的方法。 该方法可以包括在CMOS器件上形成微孔。 微孔可以包括底表面和侧壁。该方法还可以包括施加第一化学物质以选择性地附着到微孔的底表面，在微孔的侧壁上形成金属氧化物层，并施加第二种化学物质 选择性地连接到微孔的侧壁。 第二种化学物质可能与第一种化学物质不具有亲和力。

公开(公告)号：WO2012031234A2

公开(公告)日：2012-03-08

申请号：PCT/US2011050389

申请日：2011-09-02

Applicant: LIFE TECHNOLOGIES CORP ,  LI SHIFENG ,  LAFFERTY MICHAEL W ,  KOTSEROGLOU THEO ,  OLDHAM MARK

Inventor： LI SHIFENG ,  LAFFERTY MICHAEL W ,  KOTSEROGLOU THEO ,  OLDHAM MARK

IPC: B01J19/00 ,  B01L3/00 ,  G01N21/64

CPC classification number: G01N21/648 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/168 ,  G01N21/6454

Abstract: A device includes a substrate having a refractive index of at least 1.45 and a surface layer disposed over a major surface of the substrate and defining a plurality of reaction volumes. The surface layer has a refractive index in a range of 1.32 to 1.38.

Abstract translation: 一种器件包括折射率至少为1.45的衬底和设置在衬底的主表面上并限定多个反应体积的表面层。 表面层的折射率在1.32〜1.38的范围内。