公开(公告)号：WO2012174119A3

公开(公告)日：2013-04-11

申请号：PCT/US2012042263

申请日：2012-06-13

Applicant: LIFE TECHNOLOGIES CORP ,  JI YONGMEI ,  SHIH SHEUNG-MEI RITA ,  DEGORICIJA LOVORKA ,  ZHOU ZHAOHUI ,  CUMMINGS CRAIG ,  FURTADO MANOHAR ,  BRZOSKA PIUS ,  BURRELL ANGELA ,  LEONG HARRISON ,  FANG XINGWANG ,  BOUNPHENG MANGKEY ,  SHAH ROHAN

Inventor： JI YONGMEI ,  SHIH SHEUNG-MEI RITA ,  DEGORICIJA LOVORKA ,  ZHOU ZHAOHUI ,  CUMMINGS CRAIG ,  FURTADO MANOHAR ,  BRZOSKA PIUS ,  BURRELL ANGELA ,  LEONG HARRISON ,  FANG XINGWANG ,  BOUNPHENG MANGKEY ,  SHAH ROHAN

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12R1/01

Abstract: Disclosed are genomic sequences for nine strains of Cronobacter spp. (C. sakazakii - 696, 701, 680; C. malonaticus - 507, 681; C. turicensis - 564; C. muytjensii - 530; C. dublinensis - 582; C. genomospl - 581) and compositions, methods, and kits for detecting, identifying and distinguishing Cronobacter spp. strains from each other and from non-Cronobacter spp. strains. Some embodiments describe isolated nucleic acid compositions unique to certain Cronobacter strains as well as compositions that are specific to all Cronobacter spp. Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of Cronobacter spp. are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR

Abstract translation: 公开了九个克罗杆菌属菌株的基因组序列。 （C. sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组蛋白-58）和组合物，方法和试剂盒 用于检测，识别和鉴别克罗杆菌属。 菌株彼此和非克罗杆菌属。 株。 一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。 还提供引物和探针组合物以及引物和探针的使用方法。 克隆杆菌属鉴定试剂盒 也被描述。 一些实施例涉及用于设置用于PCR分析的基于控制的阈值的计算机软件方法

公开(公告)号：WO2012174119A2

公开(公告)日：2012-12-20

申请号：PCT/US2012/042263

申请日：2012-06-13

Applicant: LIFE TECHNOLOGIES CORPORATION ,  JI, Yongmei ,  SHIH, Sheung-Mei ("Rita") ,  DEGORICIJA, Lovorka ,  ZHOU, Zhaohui ,  CUMMINGS, Craig ,  FURTADO, Manohar ,  BRZOSKA, Pius ,  BURRELL, Angela ,  LEONG, Harrison ,  FANG, Xingwang ,  BOUNPHENG, Mangkey ,  SHAH, Rohan

Inventor： JI, Yongmei ,  SHIH, Sheung-Mei ("Rita") ,  DEGORICIJA, Lovorka ,  ZHOU, Zhaohui ,  CUMMINGS, Craig ,  FURTADO, Manohar ,  BRZOSKA, Pius ,  BURRELL, Angela ,  LEONG, Harrison ,  FANG, Xingwang ,  BOUNPHENG, Mangkey ,  SHAH, Rohan

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12R1/01

Abstract: Disclosed are genomic sequences for nine strains of C ronobacter spp . (C. sakazakii - 696, 701, 680; C. malonaticus - 507, 681; C. turicensis - 564; C. muytjensii - 530; C. dublinensis - 582; C. genomospl - 581) and compositions, methods, and kits for detecting, identifying and distinguishing C ronobacter spp . strains from each other and from non-C ronobacter spp . strains. Some embodiments describe isolated nucleic acid compositions unique to certain C ronobacter strains as well as compositions that are specific to all C ronobacter spp . Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of C ronobacter spp . are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR

Abstract translation: 公开了九个克罗杆菌属菌株的基因组序列。 （C. sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组蛋白-58）和组合物，方法和试剂盒 用于检测，识别和鉴别克罗杆菌属。 菌株彼此和非克罗杆菌属。 株。 一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。 还提供引物和探针组合物以及引物和探针的使用方法。 克隆杆菌属鉴定试剂盒 也被描述。 一些实施例涉及用于设置用于PCR分析的基于控制的阈值的计算机软件方法

公开(公告)号：WO2012083189A2

公开(公告)日：2012-06-21

申请号：PCT/US2011/065535

申请日：2011-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LI, Bin ,  LAO, Kai Qin ,  O'NEIL, Jennifer ,  KUNKEL, Jennifer ,  HALEY, Kellie ,  KASINSKAS, Rachel ,  MA, Zhaochun ,  BRZOSKA, Pius

Inventor： LI, Bin ,  LAO, Kai Qin ,  O'NEIL, Jennifer ,  KUNKEL, Jennifer ,  HALEY, Kellie ,  KASINSKAS, Rachel ,  MA, Zhaochun ,  BRZOSKA, Pius

IPC: C12Q1/68

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

Abstract translation: 提供了在支持物上产生固定化克隆扩增子的局部群体的新方法。

公开(公告)号：WO2011082325A3

公开(公告)日：2011-08-25

申请号：PCT/US2010062539

申请日：2010-12-30

Applicant: LIFE TECHNOLOGIES CORP ,  VATTA PAOLO ,  BRZOSKA PIUS ,  NEWTON ELIZABETH ,  PETRAUSKENE OLGA ,  FURTADO MANOHAR ,  CUMMINGS CRAIG ,  BARKER MELISSA

Inventor： VATTA PAOLO ,  BARKER MELISSA

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C07K14/245 ,  C12Q1/689 ,  C12Q2600/156

Abstract: Disclosed is the genomic sequence for E. coli O55:H7 as well as compositions, methods, and kits for detecting, identifying and distinguishing E. coli O55:H7 from non-O55:H7 strains. In some embodiments, isolated nucleic acid compositions unique and/or specific to E. coli O55:H7 are described. Methods of detection and/or indentifying E. coli O55:H7 comprising detecting at least one nucleic acid sequences comprising or derived from SEQ ID NO:1-5, SEQ ID NO: 66, SEQ ID NO: 252, SEQ ID NO: 1113, and SEQ ID NO: 1461, are described. Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of E. coli O55:H7 are also described.

Abstract translation: 公开了大肠杆菌O55：H7的基因组序列以及用于从非O55：H7菌株检测，鉴定和区分大肠杆菌O55：H7的组合物，方法和试剂盒。 在一些实施方案中，描述了对大肠杆菌O55：H7独特和/或特异性的分离的核酸组合物。 检测和/或鉴定大肠杆菌O55：H7的方法，包括检测至少一个包含或源自SEQ ID NO：1-5，SEQ ID NO：66，SEQ ID NO：252，SEQ ID NO：1113的核酸序列 ，和SEQ ID NO：1461。 还提供引物和探针组合物以及引物和探针的使用方法。 还描述了用于鉴定大肠杆菌O55：H7的试剂盒。

公开(公告)号：WO2011053871A2

公开(公告)日：2011-05-05

申请号：PCT/US2010/054867

申请日：2010-10-29

Applicant: LIFE TECHNOLOGIES CORPORATION ,  PETRAUSKENE, Olga ,  BRZOSKA, Pius ,  BIT, Somaya ,  LIU, Jen-Kuei ,  TEBBS, Robert ,  FURTADO, Manohar

Inventor： PETRAUSKENE, Olga ,  BRZOSKA, Pius ,  BIT, Somaya ,  LIU, Jen-Kuei ,  TEBBS, Robert ,  FURTADO, Manohar

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

Abstract translation: 公开了利用与单个容器中的样品接触的多个寡核苷酸引物并检测扩增产物的用于检测支原体物种和紧密相关物种的多引物扩增测定法，方法和试剂盒，其中 扩增产物的存在表明样品中的支原体。

公开(公告)号：WO2008103770A3

公开(公告)日：2008-08-28

申请号：PCT/US2008/054477

申请日：2008-02-20

Applicant: APPLERA CORPORATION ,  SHEWALE, Jaiprakash ,  FURTADO, Manohar ,  BRZOSKA, Pius ,  BARBISIN, Maura ,  FANG, Rixun ,  MALICDEM, Michael ,  O'SHEA, Cristin

Inventor： SHEWALE, Jaiprakash ,  FURTADO, Manohar ,  BRZOSKA, Pius ,  BARBISIN, Maura ,  FANG, Rixun ,  MALICDEM, Michael ,  O'SHEA, Cristin

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

Abstract: The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.

公开(公告)号：EP2721170A2

公开(公告)日：2014-04-23

申请号：EP12727766.3

申请日：2012-06-13

Applicant: Life Technologies Corporation ,  Ji, Yongmei ,  Shih, Sheung-mei ,  Degoricija, Lovorka ,  Zhou, Zhaohui ,  Cummings, Craig ,  Furtado, Manohar ,  Brzoska, Pius ,  Burrell, Angela ,  Leong, Harrison ,  Fang, Xingwang ,  Bounpheng, Mangkey ,  Shah, Rohan

Inventor： JI, Yongmei ,  SHIH, Sheung-Mei ("Rita") ,  DEGORICIJA, Lovorka ,  ZHOU, Zhaohui ,  CUMMINGS, Craig ,  FURTADO, Manohar ,  BRZOSKA, Pius ,  BURRELL, Angela ,  LEONG, Harrison ,  FANG, Xingwang ,  BOUNPHENG, Mangkey ,  SHAH, Rohan

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12R1/01

Abstract: Disclosed are genomic sequences for nine strains of C
ronobacter spp . (C.
sakazakii - 696, 701, 680; C.
malonaticus - 507, 681; C.
turicensis - 564; C.
muytjensii - 530; C.
dublinensis - 582; C.
genomospl - 581) and compositions, methods, and kits for detecting, identifying and distinguishing C
ronobacter spp . strains from each other and from non-C
ronobacter spp . strains. Some embodiments describe isolated nucleic acid compositions unique to certain C
ronobacter strains as well as compositions that are specific to all C
ronobacter spp . Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of C
ronobacter spp . are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR

公开(公告)号：WO2013029021A1

公开(公告)日：2013-02-28

申请号：PCT/US2012/052383

申请日：2012-08-24

Applicant: LIFE TECHNOLOGIES CORPORATION ,  TEBBS, Robert ,  CUMMINGS, Craig ,  BRZOSKA, Pius ,  MATHENY, Sharon ,  O'CONNELL, Catherine ,  FURTADO, Manohar ,  FANG, Rixun ,  PETRAUSKENE, Olga ,  LIEW, Sueh-Ning ,  SCHUMAKER, Michael

Inventor： TEBBS, Robert ,  CUMMINGS, Craig ,  BRZOSKA, Pius ,  MATHENY, Sharon ,  O'CONNELL, Catherine ,  FURTADO, Manohar ,  FANG, Rixun ,  PETRAUSKENE, Olga ,  LIEW, Sueh-Ning ,  SCHUMAKER, Michael

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/689 ,  C12Q2600/16

Abstract: The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin ( stx ) or an eae . In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.

Abstract translation: 本教导描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子（例如志贺毒素（stx）或eae）的一种或多种微生物。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体菌株和血清型。 一些实施方案描述了用于检测STEC微生物的组合物，方法和试剂盒。 还描述了用于多个微生物检测和鉴定的工作流程。

公开(公告)号：WO2012083189A3

公开(公告)日：2012-08-23

申请号：PCT/US2011065535

申请日：2011-12-16

Applicant: LIFE TECHNOLOGIES CORP ,  LI BIN ,  LAO KAI QIN ,  O'NEIL JENNIFER ,  KUNKEL JENNIFER ,  HALEY KELLIE ,  KASINSKAS RACHEL ,  MA ZHAOCHUN ,  BRZOSKA PIUS

Inventor： LI BIN ,  LAO KAI QIN ,  O'NEIL JENNIFER ,  KUNKEL JENNIFER ,  HALEY KELLIE ,  KASINSKAS RACHEL ,  MA ZHAOCHUN ,  BRZOSKA PIUS

IPC: C12Q1/68

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

Abstract translation: 提供了在载体上产生固定化克隆扩增子的局部群体的新方法。

公开(公告)号：WO2011053871A3

公开(公告)日：2011-09-29

申请号：PCT/US2010054867

申请日：2010-10-29

Applicant: LIFE TECHNOLOGIES CORP ,  PETRAUSKENE OLGA ,  BRZOSKA PIUS ,  BIT SOMAYA ,  LIU JEN-KUEI ,  TEBBS ROBERT ,  FURTADO MANOHAR

Inventor： PETRAUSKENE OLGA ,  BRZOSKA PIUS ,  BIT SOMAYA ,  LIU JEN-KUEI ,  TEBBS ROBERT ,  FURTADO MANOHAR

IPC: C12Q1/68 ,  C12P19/34 ,  G01N33/48

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

Abstract translation: 公开了一种多引物扩增测定法，用于利用在单个容器中与样品接触的多个寡核苷酸引物检测支原体物种和密切相关物种的检测扩增产物，其中扩增产物的存在表示支原体 在样品中。