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61.发明公开DNA 마커인 RM18166, RM18171 및 Indel 15040을 포함하는 끝동매미충 저항성 유전자 Ｇrh1을 가진 벼 품종 선별용 조성물 有权用于选择含有DNA标记RM18166，RM18171和Indel 15040的含有Grh1基因的绿色稻叶蝉抗性品种的组合物
公开(公告)号：KR1020150020492A
公开(公告)日：2015-02-26
申请号：KR1020130097495
申请日：2013-08-16
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q1/6895 , C12Q2600/13
Abstract: The present invention relates to a composition for selecting a rice breed for selecting a rice breed having leafhopper-resistance. The composition includes a primer which detects at least one oligonucleotide or a fraction thereof selected from a group consisting of RM18166, RM18171, and Indel15040 as DNA markers. More specifically, the composition includes one or more primer sets selected from a group consisting of SEQ ID: 1-6. In addition, the present invention relates to a method and a kit for selecting a rice breed having leafhopper-resistance. The method includes the steps of: separating genome DNAs from a rice sample; amplifying a target sequence by performing amplification reaction using one or more primer sets which uses the separated genome DNAs as a template and is selected from a group consisting of SEQ ID: 1-6; and detecting the amplified product. When the method of the present invention is used, rice individuals which have leafhopper-resistance and sensitivity can be efficiently identified by using the makers, which are very closely related to locations of leafhopper-resistant gene.
Abstract translation: 本发明涉及用于选择具有叶蝉抗性的水稻品种的水稻品种的组合物。 组合物包括检测至少一种选自RM18166，RM18171和Indel15040的寡核苷酸或其部分作为DNA标记的引物。 更具体地，组合物包含选自SEQ ID：1-6所示的一种或多种引物组。 此外，本发明涉及一种用于选择具有叶蝉抗性的水稻品种的方法和试剂盒。 该方法包括以下步骤：从水稻样品中分离基因组DNA; 通过使用使用分离的基因组DNA作为模板的一个或多个引物组进行扩增反应来扩增靶序列，并且选自SEQ ID：1-6; 并检测扩增产物。 当使用本发明的方法时，可以通过使用与叶蝉抗性基因的位置非常密切相关的制造者有效地鉴定具有叶蝉抗性和敏感性的水稻个体。
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62.发明授权눈큰흑찰(흑찰거대배아미) 현미 또는 이의 추출물을 포함하는 황반변성의 예방 또는 치료용 조성물 有权包含Nunkeunhukchal（黑色糯米与巨胚）的提取物或糙米用于预防或治疗黄斑变性的组合物
公开(公告)号：KR101483581B1
公开(公告)日：2015-01-16
申请号：KR1020130058646
申请日：2013-05-23
Applicant: 대한민국(농촌진흥청장)
IPC: A61K36/899 , A61P27/02 , A61P27/00
Abstract: 본 발명은 흑찰거대배아미(눈큰흑찰) 및 이의 추출물을 유효성분으로 포함하는, 황반변성의 예방 또는 치료용 조성물 및 루테인이 강화된 식품 조성물에 관한 것이다.
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63.发明公开눈큰흑찰(흑찰거대배아미) 현미의 추출물로부터 비타민 E를 제조하는 방법 및 상기 추출물을 포함하는 조성물 无效用于制备维生素E的方法，包括提取物（NUNKEUNHUKCHAL）（黑色大豆和大豆胚芽））和包含提取物的组合物
公开(公告)号：KR1020140137757A
公开(公告)日：2014-12-03
申请号：KR1020130058644
申请日：2013-05-23
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A23L33/10 , A23L7/10 , A23L33/105 , A23L33/15
Abstract: 본 발명은 흑찰거대배아미(눈큰흑찰)의 추출물로부터 비타민 E를 제조하는 방법, 및 흑찰거대배아미 또는 이의 추출물을 유효성분으로 포함하는, 비타민 E가 강화된 식품 조성물에 관한 것이다.
Abstract translation: 本发明涉及一种由具有巨胚的黑色糯米（Nunkeunhukchal）的提取物制备维生素E的方法，以及含有具有巨型胚的黑色糯米或其提取物作为活性成分的具有增强维生素E的食品组合物。 更具体地，提供了一种制备维生素E的方法，包括通过使用选自水，具有1至4个碳原子的醇，己烷和乙酸乙酯的溶剂来提取具有巨胚的黑色粘米的步骤。
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公开(公告)号：KR1020140046570A
公开(公告)日：2014-04-21
申请号：KR1020120110742
申请日：2012-10-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/686 , C12Q2600/13
Abstract: The present invention provides: a primer set for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; a kit for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; and a method of differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors by performing an amplification reaction using the primer set. By using the primer set of the present invention, a user can differentiate black rice in a seedling condition compared to an existing sorting method of confirming the color of seed coats after the vegetative growth, discharging water, and grain filling; and the homomorphy and heteromorphy of characteristics can be possible, thereby improving the breeding efficiency utilizing the juvenile selection of black rice and minimum resources. [Reference numerals] (AA) Boseokheugchal(black rice); (BB) No.244 of Miryang(white rice); (CC) Approximately 450 bp; (DD) Approximately 215 bp
Abstract translation: 本发明提供了一种用于分化含有花青素和其他糙米种衣色的黑米的种皮颜色的引物组; 一种用于区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的试剂盒; 以及通过使用引物组进行扩增反应来区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的方法。 通过使用本发明的引物组，与用于确认营养生长后的种皮颜色的现有分类方法相比，使用者能够在苗期条件下区分黑米，排出水和灌浆; 特色的同性异体和异质性是可能的，从而通过青少年选择黑米和最低限度的资源提高育种效率。 （AA）Boseokheugchal（黑米）; （BB）米阳244号（白米）; （CC）约450bp; （DD）约215bp
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65.发明授权ＤＮＡ 마커를 포함하는 끝동매미충 저항성 유전자 Ｇｒｈ１을 가진 벼 품종 선별용 조성물 및 ＤＮＡ 마커를 이용한 끝동매미충 저항성 벼 품종 선별 방법 有权用于选择含有DNA标记的含有Grh1基因的绿色稻叶蝉抗性品种的组合物和使用DNA标记选择绿色水稻叶hop抗性品种的方法
公开(公告)号：KR101322345B1
公开(公告)日：2013-10-28
申请号：KR1020110030389
申请日：2011-04-01
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 RM516, RM6082, RM18182, RM18184, RM18185, RM5844 및 RM7409로 구성되는 군으로부터 선택된 하나 이상의 올리고뉴클레오티드 또는 이의 단편의 존재여부를 검출하는 프라이머를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물, 구체적으로는, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물에 관한 것이다. 또한, 본 발명은 벼 시료에서 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 이용하여 증폭 반응을 수행하여 표적 서열을 증폭하는 단계; 및 상기 증폭 산물을 검출하는 단계를 포함하는, 끝동매미충 저항성 벼 품종 선별 방법 및 벼 선별 키트에 관한 것이다. 본 발명에 따른 끝동매미충 저항성 개체를 선발하는 방법을 이용하면, 끝동매미충 저항성 유전자의 위치와 아주 밀접하게 연관된 마커를 이용하여 효율적으로 끝동매미충 저항성 및 감수성 개체를 구분할 수 있다.
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Patent Agency Ranking66.发明公开
公开(公告)号：KR1020130091434A
公开(公告)日：2013-08-19
申请号：KR1020120012710
申请日：2012-02-08
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12Q1/6844 , C12Q2600/13 , C12Q2600/158
Abstract: PURPOSE: A method of selecting a breed that is resistive to a rice stripe disease is provided to efficiently separate a breed that is resistive and sensitive with respect to the rice stripe disease by using a marker, which is closely related with a location of a rice stripe disease-resistant gene. CONSTITUTION: A pair of primers for selecting a breed of rice resistive against a rice stripe disease is selected from the group consisting of a primer pair represented by SEQ ID NO: 1 and 2; a primer pair represented by SEQ IE NO: 3 and 4; a primer pair represented by SEQ IE NO: 5 and 6; a primer pair represented by SEQ IE NO: 7 and 8; a primer pair represented by SEQ IE NO: 9 and 10; a primer pair represented by SEQ IE NO: 11 and 12; a primer pair represented by SEQ IE NO: 13 and 14; a primer pair represented by SEQ IE NO: 15 and 16; a primer pair represented by SEQ IE NO: 17 and 18; a primer pair represented by SEQ IE NO: 19 and 20; and a primer pair represented by SEQ IE NO: 21 and 22. A method of selecting a rice stripe disease-resistant breed comprises the steps of: (i) isolating DNA from a rice sample; (ii) amplifying a target sequence by performing an amplification reaction by having the isolated DNA as a template and using the at least one primer pair selected from above; and (iii) detecting the amplification product.
Abstract translation: 目的：提供一种选择抗稻瘟病抗性品种的方法，通过使用与稻米位置密切相关的标记，有效地分离出与水稻条纹病有关的抗性和敏感性品种 条纹抗病基因。 构成：从由SEQ ID NO：1和2表示的引物对组成的组中选择一对用于选择抗稻瘟病抗性的水稻品种的引物; 由SEQ ID NO：3和4表示的引物对; 由SEQ ID NO：5和6表示的引物对; 由SEQ ID NO：7和8表示的引物对; 由SEQ ID NO：9和10表示的引物对; 由SEQ ID NO：11和12表示的引物对; 由SEQ ID NO：13和14表示的引物对; 由SEQ ID NO：15和16表示的引物对; 由SEQ ID NO：17和18表示的引物对; 由SEQ ID NO：19和20表示的引物对; 和由SEQ ID NO：21和22表示的引物对。选择水稻条纹抗病品种的方法包括以下步骤：（i）从稻样品中分离DNA; （ii）通过使分离的DNA作为模板并使用选自上述的至少一种引物对进行扩增反应来扩增靶序列; 和（iii）检测扩增产物。
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67.发明授权
公开(公告)号：KR101278600B1
公开(公告)日：2013-06-25
申请号：KR1020110146547
申请日：2011-12-29
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A23L7/115 , A23L5/32 , A23V2002/00 , A23V2300/14 , A23V2300/48
Abstract: PURPOSE: A method of effectively producing GABA(Gamma-aminobutyric acid) and amino acid and a composition thereof are provided to maximize the production of GABA and amino acid from unpolished rice of giant embryonic rice and rice bran. CONSTITUTION: Rice bran of giant embryonic rice Milyang No. 263 is mixed with a solvent and extracted by ultrasonic waves. The solvent used in the extraction is water, C1-C4 alcohol, ethyl acetate, acetone, or a mixed solvent thereof. [Reference numerals] (AA) Properties; (BB) Varieties; (CC) Ordinary rice; (DD) Superb rice; (EE) Black glutinous rice; (FF) Early maturing black glutinous rice; (GG) Giant embryonic rice; (HH) Embryo; (II) Giant embryonic glutinous rice; (JJ) Giant black glutinous rice; (KK) Mylyang No. 263
Abstract translation: 目的：提供有效生产GABA（γ-氨基丁酸）和氨基酸及其组合物的方法，以最大限度地利用巨型胚胎米和米糠未糙米的GABA和氨基酸的产生。 构成：将巨型胚胎米苗263号的米糠与溶剂混合并通过超声波提取。 萃取中使用的溶剂是水，C1-C4醇，乙酸乙酯，丙酮或其混合溶剂。 （附图标记）（AA）性质; （BB）品种; （CC）普通米; （DD）精湛的米饭; （EE）黑糯米; （FF）早熟黑糯米; （GG）巨型大米; （HH）胚胎; （二）大型糯米糯米; （JJ）巨型黑糯米; （KK）Mylyang No.263
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公开(公告)号：KR1020120037585A
公开(公告)日：2012-04-20
申请号：KR1020100099131
申请日：2010-10-12
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A cosmetic composition containing black waxy rice extract is provided to ensure excellent anti-wrinkling and skin whitening. CONSTITUTION: A cosmetic composition for anti-wrinkling and skin whitening contains 0.01-10 wt% of black waxy rice extract as an active ingredient. The extract is prepared using water, ethanol, methanol, or ethyl acetate. The extract contains anthocyanine, amylo pectin, GABA, gamma-oryzanol, proteins, fat and fatty acid, inorganic ingredients, amino acids, saccharides, and vitamins.
Abstract translation: 目的：提供含有黑糯米提取物的化妆品组合物，以确保优异的抗皱和皮肤美白。 构成：抗皱和皮肤美白化妆品组合物含有0.01-10重量％的黑糯米提取物作为活性成分。 萃取液用水，乙醇，甲醇或乙酸乙酯制备。 提取物含有花青，淀粉果胶，GABA，γ-谷维素，蛋白质，脂肪和脂肪酸，无机成分，氨基酸，糖类和维生素。
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公开(公告)号：KR1020170056090A
公开(公告)日：2017-05-23
申请号：KR1020150159108
申请日：2015-11-12
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은삼강벼내한발성분자마커및 이의용도에관한것으로, 보다구체적으로, 본발명은삼강벼내한발성분자마커, 상기분자마커를검출할수 있는프로브또는증폭할수 있는프라이머를포함하는내한발성삼강벼선별용조성물, 상기조성물을포함하는내한발성삼강벼선별용키트및 상기삼강벼내한발성분자마커를검출하는단계를포함하는내한발성삼강벼선별방법에관한것이다. 본발명에서제공하는삼강벼내한발성분자마커를이용하면내한발성벼 계통및 품종의육종효율을증대할수 있고내한발성대량생물검정을위한투자비용을절감할수 있다.
Abstract translation: 本发明涉及一种冷samgangbyeo发声分子标记及其用途，更具体地，本发明samgangbyeo冷发声分子标记，冷发声samgangbyeo筛选组合物，包括引物，可以探测或扩增可检测的分子标记 ，含有该组合物的耐冷三联体选择试剂盒和三重冷驻留分子标记物。 与samgangbyeo冷挥发性分子标志物的本发明所提供的，并能增加的育种系和品种稻谷的效率也可以是冷灾区减少遭致质量生物测定冷的投资成本。
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70.发明公开벼 유래의 고친화성 인산운반체 OsPT4 유전자가 과발현된 항생제 마커프리 형질전환 벼 有权抗生素无标记的转基因大米，具有超高表达的高亲和力磷酸盐转运蛋白OSPT4基因ORYZA SATIVA
公开(公告)号：KR1020160059103A
公开(公告)日：2016-05-26
申请号：KR1020140160405
申请日：2014-11-17
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/82 , A01H5/00 , C12N15/8201
Abstract: 본발명은식물체형질전환에관한기술로서, 구체적으로벼 유래의고친화성인산운반체유전자인 OsPT4가도입되고항생제선발마커가제거된형질전환벼 및이의제조방법에관한것이다. 본발명의형질전환벼는항생제선발마커가들어있지않아 GM작물에대한소비자들의안전성에대한우려를경감하여상업적인활용이타 형질전환벼에비해우수하다. 또한, 본발명에사용된 OsPT4은인산결핍시벼에서발현되는고친화성인산운반체로서, 상기유전자의과발현을통해식물이필요로하는필수영양소중의하나인인산흡수를증진시킴으로써토양인산이용성을높일수 있다.
Abstract translation: 本发明涉及与植物转化相关的技术。 更具体地说，本发明涉及一种具有抗生素选择标记的转基因水稻，并且其中引入了由水稻衍生的高亲和性磷酸酯载体基因OsPT4。 本发明还涉及其制备方法。 不包括抗生素选择标记的转基因水稻，对消费者对转基因作物的安全性的担忧降低，与其他转基因水稻相比，转基因水稻确保优良的商业适用性。 此外，本发明中使用的OsPT4是在磷酸盐缺乏的条件下在水稻中表达的高亲和力磷酸盐载体。 因此，基因的过表达可以增加磷酸盐的摄取，磷酸盐是植物必需营养素之一，从而提高磷酸盐在土壤中的利用率。
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