公开(公告)号：MX2013008988A

公开(公告)日：2014-03-21

申请号：MX2013008988

申请日：2012-02-02

Applicant: GENOMATICA INC

Inventor： OSTERHOUT ROBIN E ,  PHARKYA PRITI ,  BURK MARK J ,  BURGARD ANTHONY P ,  SUNJUN

IPC: C12P5/02

Abstract: La invención provee organismos microbianos no naturales que tienen una ruta de butadieno. Adicionalmente, la invención provee métodos de uso de dichos organismos para producir butadieno. En algunos aspectos las modalidades descritas en la presente se refieren a un procedimiento de producción de butadieno que incluye: (a) cultivar por fermentación, en una cantidad suficiente de nutrientes y medio, un organismo microbiano no natural que produce alcohol crotílico; y (b) convertir en butadieno el alcohol crotílico producido cultivando el organismo microbiano no natural.

公开(公告)号：CA2882896A1

公开(公告)日：2014-03-06

申请号：CA2882896

申请日：2013-08-27

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  OSTERHOUT ROBIN E ,  VAN DIEN STEPHEN J ,  TRACEWELL CARA ANN ,  PHARKYA PRITI ,  ANDRAE STEFAN

IPC: C12P7/18 ,  C07C31/04 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/52 ,  C12N15/53 ,  C12N15/54 ,  C12N15/55 ,  C12N15/60 ,  C12P7/14 ,  C12P7/16

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：PH12013502032A1

公开(公告)日：2014-01-06

申请号：PH12013502032

申请日：2013-09-30

Applicant: GENOMATICA INC

Inventor： BURK MARK J ,  BURGARD ANTHONY P ,  OSTERHOUT ROBIN E ,  SUN JUN ,  PHARKYA PRITI

IPC: C12P7/40

Abstract: The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2- hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.

公开(公告)号：ZA201204217B

公开(公告)日：2013-08-28

申请号：ZA201204217

申请日：2012-06-08

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  BURK MARK J ,  PHARKYA PRITI

IPC: C12P20060101 ,  C12N20060101

Abstract: A non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway includes at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme or protein expressed in a sufficient amount to produce 1,3-BDO. A method for producing 1,3-BDO that includes culturing the this non-naturally occurring microbial organism under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：AU2013203175A9

公开(公告)日：2013-06-20

申请号：AU2013203175

申请日：2013-04-09

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  OSTERHOUT ROBIN E ,  SUN JUN ,  PHARKYA PRITI

IPC: C12P7/04 ,  C12N1/21

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, a 6-aminocaproic acid, caprolactam, or hexametheylenediamine pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine pathway and at least one exogenous nucleic acid that encodes an enzyme that increases the yields of adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine by (i) enhancing carbon fixation via the reductive TCA cycle, and/or (ii) accessing additional reducing equivalents from gaseous carbon sources and/or syngas components such as CO, C02, and/or H2. The invention additionally provides a method for producing adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine. The method can include culturing an adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism described herein under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine. Adipat Adipy oA Addi Co ylC A dC 5-C xy-2Mpentn4ylA mico'. 4,2,1 ,17 34Hydroxyadipyl-CoA s c~40 3 -Oxoai pyl-CA4A ooQ 4 4 ~ ~ ~ ~ , - C 'o A 4 ~ Y . . " " " " " ' 4~ Acety~oA CWsa-mkase 134* Succinyl-CoAk "0yA-e Aey-o

公开(公告)号：AU2013203764A1

公开(公告)日：2013-05-30

申请号：AU2013203764

申请日：2013-04-11

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  BURK MARK J ,  OSTERHOUT ROBIN E ,  PHARKYA PRITI ,  LI JINGYI

IPC: C12N15/00

Abstract: Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway. and methods of using such organisms to produce 1,3-BDO.

公开(公告)号：AU2013203163A1

公开(公告)日：2013-05-02

申请号：AU2013203163

申请日：2013-04-09

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  OSTERHOUT ROBIN E ,  SUN JUN ,  PHARKYA PRITI

IPC: C12P7/04 ,  C12N1/12

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4 hydroxybutyryl-CoA, 4- hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4 hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine. CD, -CC C,) I CD E! cc0) -C C)) -1 C) C:)) Sa -r 0 0 ~Cc-) C-)) C>

公开(公告)号：AU2013202923A1

公开(公告)日：2013-05-02

申请号：AU2013202923

申请日：2012-06-20

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  OSTERHOUT ROBIN E ,  SUN JUN ,  PHARKYA PRITI

IPC: C12P5/02 ,  C12N1/00 ,  C12N15/74

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols and at least one exogenous nucleic acid that encodes an enzyme that increases the yields of the primary alcohol by (i) enhancing carbon fixation via the reductive TCA cycle, and/or (ii) accessing additional reducing equivalents from gaseous carbon sources and/or syngas components such as CO, C02, and/or H2. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：AU2013202629A1

公开(公告)日：2013-05-02

申请号：AU2013202629

申请日：2013-04-05

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  BURK MARK J ,  OSTERHOUT ROBIN E ,  PHARKYA PRITI

IPC: C12N1/21 ,  C12N1/20 ,  C12P7/16 ,  C12P7/18

Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3 BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4 hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2 one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. Cl) 74 c I- o9 Co 0 MA C6 ~ 4) a CN m~ N' zI

公开(公告)号：AU2012272856A1

公开(公告)日：2013-05-02

申请号：AU2012272856

申请日：2012-06-21

Applicant: GENOMATICA INC

Inventor： BURGARD ANTHONY P ,  OSTERHOUT ROBIN E ,  SUN JUN ,  PHARKYA PRITI

IPC: C12P7/04 ,  C12N1/12

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4- hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.