公开(公告)号：WO1994024148A1

公开(公告)日：1994-10-27

申请号：PCT/US1994004069

申请日：1994-04-13

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  BOIME, Irving

IPC: C07K03/00

CPC classification number: C07K14/59 ,  A61K38/00 ,  C07K2319/75

Abstract: "Partial" and "complete" CTP units are used to modify biologically active proteins and peptides to alter their clearance patterns. "Complete" CTP units have the amino acid sequence found at positions 112-118 to position 145 of the beta -subunit of human chorionic gonadotropin; "partial" CTP units are missing at least one amino acid in the region of position 118-145 inclusive. Variants of these CTP units contain 1-5 conservative amino acid substitutions which do not destroy activity. Suitable peptides or proteins which may be modified in this manner include various hormones and cytokines.

Abstract translation: “部分”和“完整”CTP单位用于修饰生物活性蛋白质和肽以改变其清除模式。 “完全”CTP单元具有在人绒毛膜促性腺激素的β亚基的位置112-118至位置145处发现的氨基酸序列; “部分”CTP单位在118-145位置区域中缺少至少一个氨基酸。 这些CTP单元的变体含有1-5个保守氨基酸取代，其不破坏活性。 可以以这种方式修饰的合适的肽或蛋白质包括各种激素和细胞因子。

公开(公告)号：WO1994020826A1

公开(公告)日：1994-09-15

申请号：PCT/US1994002172

申请日：1994-03-01

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  DIMAROGONAS, Andrew, D.

IPC: G01H01/00

CPC classification number: G01N3/30 ,  G01H1/16 ,  G01N3/32 ,  G01N29/045 ,  G01N29/11 ,  G01N29/12 ,  G01N2203/0055 ,  G01N2203/0073 ,  G01N2203/0688 ,  G01N2291/0235 ,  G01N2291/0258 ,  G01N2291/02827 ,  G01N2291/101 ,  G01N2291/267

Abstract: The fatigue integrity of metallic materials may be determined be either one of two methods. In a first method, an impulse of energy is introduced into the metallic material, such as by striking the metallic material (20), and the induced vibration is sensed and analyzed in order to compute the damping factor thereof, the damping factor being directly related to the fatigue thereof. With this method, a transducer (28) is coupled to the metallic material and its output is amplified by an amplifier (30) before being input to a computer (32) which determines the damping factor. In a second method, a continuous energy input is provided to the metallic material, such as by utilizing a frequency generator coupled to a power amplifier whose output drives a transducer such as a speaker or the like for inducing a continuous vibration in the metallic material. This continuous vibration is measured with a transducer, an amplifier, and a damping factor calculated with a computer as in the first method.

Abstract translation: 金属材料的疲劳完整性可以通过两种方法之一来确定。 在第一种方法中，例如通过撞击金属材料（20）将金属材料中的能量脉冲引入金属材料中，感测和分析感应振动以计算其阻尼因子，阻尼因子直接相关 对其疲劳。 利用这种方法，传感器（28）耦合到金属材料，并且其输出在被输入到确定阻尼因子的计算机（32）之前由放大器（30）放大。 在第二种方法中，例如通过利用耦合到功率放大器的频率发生器提供连续的能量输入，该功率放大器的输出驱动诸如扬声器等的换能器，以引起金属材料中的连续振动。 用第一种方法用计算机计算的换能器，放大器和阻尼因子测量该连续振动。

公开(公告)号：WO1994010831A1

公开(公告)日：1994-05-26

申请号：PCT/US1993010629

申请日：1993-11-05

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  HO, Tuan-Hua, David

IPC: A01H05/00

CPC classification number: C12N15/827 ,  A01H5/10 ,  C07K14/415

Abstract: This invention relates to methods of inducing a dwarfed stature into plants by the recombinant introduction of a DNA sequence encoding a group 3 late embryogenesis abundant protein. In addition to dwarfism, the expression of this protein tends to confer early flowering, more branching, and stress resistance, in particular drought resistance, to the transformed plants.

Abstract translation: 本发明涉及通过重组引入编码第3组晚期胚胎发生丰富蛋白质的DNA序列来诱导植物矮化的方法。 除了侏儒症之外，这种蛋白质的表达倾向于赋予转化植物的早期开花，更多的支链和抗应激性，特别是抗旱性。

公开(公告)号：WO1992019248A1

公开(公告)日：1992-11-12

申请号：PCT/US1992003609

申请日：1992-05-01

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  STAHL, Philip, D.

IPC: A61K31/70

CPC classification number: C07K7/06 ,  A61K38/00 ,  C07K7/08 ,  C07K9/00 ,  C07K14/001 ,  Y02A50/478

Abstract: Polypeptide backbones containing sugar residues at repetitive intervals are capable of binding the mannose receptor when said sugars are mannosyl, fucosyl, or N-acetyl glucosamine residues. These peptides are of the formula X-(Z(S)AAn1)n2-Y (I) wherein S represents a mannose, fucose, glucose or N-acetylglucosamine residue optionally coupled to a linker moiety; Z is the residue of an amino acid to which S is coupled; each AA is independently the residue of an additional amino acid, n1 is an integer = 1, 2 or 3; n2 is 3-15, and X and Y are noninterfering substituents. They are useful in the treatment of various diseases mediated by macrophage activity and proliferation.

Abstract translation: 当所述糖是甘露糖基，岩藻糖基或N-乙酰氨基葡萄糖残基时，以重复间隔含有糖残基的多肽骨架能够结合甘露糖受体。 这些肽具有式X-（Z（S）AAn1）n2-Y（I），其中S表示任选地与接头部分偶联的甘露糖，岩藻糖，葡萄糖或N-乙酰氨基葡萄糖残基; Z是S连接的氨基酸的残基; 每个AA独立地是另外的氨基酸的残基，n1是整数= 1,2或3; n2为3-15，X和Y为非干扰取代基。 它们可用于治疗由巨噬细胞活性和增殖介导的各种疾病。

公开(公告)号：WO1992015011A1

公开(公告)日：1992-09-03

申请号：PCT/US1992001601

申请日：1992-02-24

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  LEAHEY, Harry, S. ,  BINNS, W., Robert ,  EPSTEIN, John, W. ,  KLARMANN, Joseph ,  DAVIS, Michael, J.

IPC: G01N23/16

CPC classification number: G01B15/02 ,  G01B15/025 ,  G01F23/288 ,  G01G9/005 ,  G01G17/02 ,  G01N23/16

Abstract: Apparatus for continuously determining the areal density along a length of a material includes a radioactive source and a scintillating receiver. In a first embodiment, the invention measures the thickness of a traveling web simultaneously at multiple points across its full width. The apparatus includes a radioactive source (3) which extends across one side of the web (5). A detector array (6), on the opposite side of the web, detects the emission from the source through the web. The array includes scintillating fibers and multi- or single-anode photomultipliers (9) which may be connected by a scintillating or non-scintillating fiber optic elements (7) to the photomultiplier. In a second embodiment, the invention continuously detects the fill level of a plurality of containers as they are conveyed between the receiver and detector. A scintillating fiber receiver may have its fibers arranged either vertically or horizontally. When arranged horizontally, the fibers may be grouped and conduct photons to single anodes of a multi-anode photo-multiplier tube to provide direct physical measurement of the fill level. Still another receiver includes a plastic scintillator sheet directly coupled to a photomultiplier tube.

Abstract translation: 用于连续地确定沿材料长度的面密度的装置包括放射源和闪烁接收器。 在第一实施例中，本发明在穿过其全宽度的多个点处同时测量行进幅材的厚度。 该装置包括横跨幅材（5）的一侧延伸的放射源（3）。 在幅材的相对侧上的检测器阵列（6）通过幅材检测来自源的发射。 该阵列包括闪烁纤维和可以通过闪烁或非闪烁光纤元件（7）连接到光电倍增管的多或单阳光电倍增管（9）。 在第二实施例中，当接收器和检测器之间传送时，本发明连续地检测多个容器的填充水平。 闪烁的光纤接收器可以使其光纤垂直或水平布置。 当水平布置时，纤维可以被分组并且将光子传导到多阳极光电倍增管的单个阳极，以提供填充水平的直接物理测量。 另一个接收器包括直接耦合到光电倍增管的塑料闪烁体片。

公开(公告)号：WO1991015217A1

公开(公告)日：1991-10-17

申请号：PCT/US1991002371

申请日：1991-04-05

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  TOLLEFSEN, Douglas, M. ,  MAIMONE, Margaret, M.

IPC: A61K31/725

CPC classification number: C07H13/04 ,  A61K31/70 ,  C07H15/04 ,  C08B37/0069

Abstract: Low molecular weight polysaccharides related to dermatan sulfate have been shown to be the effective minimal structures to prevent or reduce coagulation in warm blooded animals.

Abstract translation: 已显示与硫酸皮肤素有关的低分子量多糖是预防或减少温血动物凝血的有效最小结构。

公开(公告)号：WO1990012086A1

公开(公告)日：1990-10-18

申请号：PCT/US1990001689

申请日：1990-03-30

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  BOUCAUD, Jean-Luc ,  CURTISS, Roy, III ,  GENTRY-WEEKS, Claudia

IPC: C12N05/10

CPC classification number: C07K16/1225 ,  A61K39/099 ,  A61K2039/552 ,  C07K14/235

Abstract: Bordetella outer membrane proteins, vaccine compositions containing these proteins, and methods of administering the same are disclosed. The outer membrane proteins can be produced by recombinant means and expressed in an avirulent carrier microbe which may be used to immunize a subject of interest. The subject antigens provide a means for protecting against or treating Bordetella-induced diseases such as upper respiratory tract disease in turkey and chickens, atropic rhinitis in swine and dogs, and whooping cough in humans.

Abstract translation: 公开了博德氏菌外膜蛋白，含有这些蛋白质的疫苗组合物及其施用方法。 外膜蛋白可以通过重组方法产生并在无毒素载体微生物中表达，其可以用于免疫感兴趣的受试者。 受试抗原提供了一种防止或治疗博德特氏杆菌诱发的疾病如火鸡和鸡的上呼吸道疾病，猪和狗的躁狂性鼻炎以及百日咳在人中的手段。

公开(公告)号：WO1990011688A1

公开(公告)日：1990-10-18

申请号：PCT/US1990001603

申请日：1990-03-26

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  CURTISS III, Roy

IPC: A01N63/00

CPC classification number: C12R1/42 ,  A61K39/0275 ,  C12N15/00 ,  C12N15/74 ,  Y02A50/482

Abstract: This invention provides a vaccine for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of S. choleraesuis. The derivatives being substantially incapable of producing functional adenylate cyclase and/or cyclic AMP receptor protein. The invention also provides a vaccine for the immunization of a vertebrate and invertebrate comprising a virulent derivative of a pathogenic microbe said derivative being substantially incapable of producing functional adenylate cyclase and/or cyclic AMP receptor protein while being capable of expressing a recombinant gene derived from a pathogen of said vertebrate to produce an antigen capable of inducing an immune response in said vertebrate against said pathogen.

Abstract translation: 本发明提供了用于免疫脊椎动物或无脊椎动物的疫苗，其包含霍乱病毒的无毒性衍生物。 该衍生物基本上不能产生功能性腺苷酸环化酶和/或环AMP受体蛋白。 本发明还提供了用于免疫脊椎动物和无脊椎动物的疫苗，其包含病原微生物的有毒衍生物，所述衍生物基本上不能产生功能性腺苷酸环化酶和/或环AMP受体蛋白质，同时能够表达源自 所述脊椎动物的病原体产生能够在所述脊椎动物中针对所述病原体诱导免疫应答的抗原。

公开(公告)号：WO1998038312A1

公开(公告)日：1998-09-03

申请号：PCT/US1997003106

申请日：1997-02-28

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  MECHAM, Robert, Paul ,  PARK, Pyong, Woo

IPC: C12N15/31

CPC classification number: C07K14/31 ,  A61K38/00

Abstract: A microbial surface component recognizing adhesive matrix molecules (MSCRAMM) is disclosed which is characterized by its ability to bind to elastin, its inhibited activity in the presence of SDS and its enhanced activity in the presence of thiol reductants. More particularly, the MSCRAMM is bacterial in origin and is a member of the family of elastin binding proteins. In one embodiment, the MSCRAMM comprises a polypeptide having an amino acid sequence defined herein as SEQ ID NO:2, and extends to active fragments thereof. The role of MSCRAMMs in bacterial infection and its sequelae and related conditions is noted and the MSCRAMM may be prepared and used in diagnostic procedures and tests, including drug discovery assays, as well as in pharmaceutical compositions applicable for corresponding therapeutic methods. Both agonists and antagonists of the MSCRAMM are proposed and illustrated.

Abstract translation: 公开了识别粘合基质分子（MSCRAMM）的微生物表面组分，其特征在于其结合弹性蛋白的能力，其在SDS存在下的抑制活性及其在硫醇还原剂存在下的增强的活性。 更具体地说，MSCRAMM是来自细菌的，并且是弹性蛋白结合蛋白家族的成员。 在一个实施方案中，MSCRAMM包含具有如SEQ ID NO：2所定义的氨基酸序列的多肽，并延伸至其活性片段。 注意到MSCRAMM在细菌感染及其后遗症和相关病症中的作用，并且MSCRAMM可以制备并用于诊断程序和测试，包括药物发现测定以及适用于相应治疗方法的药物组合物中。 提出并说明了MSCRAMM的激动剂和拮抗剂。

公开(公告)号：WO1998018128A1

公开(公告)日：1998-04-30

申请号：PCT/US1997019201

申请日：1997-10-23

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  O'SULLIVAN, Joseph, Andrew ,  INDECK, Ronald, Scott ,  MULLER, Marcel, Wettstein

IPC: G11B27/36

CPC classification number: G11B27/36 ,  G11B5/00 ,  G11B5/035 ,  G11B5/09 ,  G11B5/488 ,  G11B20/182 ,  G11B20/24 ,  G11B2005/001 ,  G11B2005/0026

Abstract: Electronics (40) implements the multiplicative noise model of the present invention. The diagnostic signal generator (50) processes the data signal s(t) to produce a diagnostic signal. The medium noise estimator (60) predicts repeatable noise components based on analysis of the diagnostic signal. Using the estimate of the medium noise, the precompensated signal generator (70) produces a corrected write signal.

Abstract translation: 电子（40）实现本发明的乘法噪声模型。 诊断信号发生器（50）处理数据信号s（t）以产生诊断信号。 中噪声估计器（60）基于诊断信号的分析来预测可重复的噪声分量。 使用中值噪声的估计，预补偿信号发生器（70）产生校正的写入信号。