公开(公告)号：WO2011112749A3

公开(公告)日：2012-01-12

申请号：PCT/US2011027790

申请日：2011-03-09

Applicant: LIFE TECHNOLOGIES CORP ,  NEUJAHR DAVID ,  MOHAMMED AMINU ,  ROSSIO JEFFREY

Inventor： NEUJAHR DAVID ,  MOHAMMED AMINU ,  ROSSIO JEFFREY

IPC: G01N33/68 ,  G01N33/53 ,  G01N33/533

CPC classification number: G01N33/6893 ,  G01N33/6884 ,  G01N2333/521 ,  G01N2333/54 ,  G01N2333/5437 ,  G01N2800/12 ,  G01N2800/245

Abstract: Described and disclosed are methods for determining, predicting, diagnosing, treating, and monitoring lung diseases, including bronchiolitis obliterans syndrome and acute cellular rejection in a lung transplant recipient by measuring chemokine levels in bronchoalveolar lavage (BAL) samples. The chemokine CXCL10 is measured in combination with at least one analyte selected from the group consisting of IL1RA, CXCL11, MCP-1, CXCL9, RANTES, IL-13, IL-17, IL-22, fractalkine, and eotaxin; and/or biomarkers. The present teachings also relate to methods, treatment decisions and kits for detecting and monitoring onset of lung transplant rejection in advance of clinically recognized symptoms.

Abstract translation: 描述和公开了通过测量支气管肺泡灌洗（BAL）样品中的趋化因子水平来确定，预测，诊断，治疗和监测肺部疾病的方法，包括闭塞性细支气管炎综合征和肺移植受体中的急性细胞排斥反应。 与至少一种选自IL1RA，CXCL11，MCP-1，CXCL9，RANTES，IL-13，IL-17，IL-22，fractalkine和嗜酸细胞活化趋化因子的分析物组合测量趋化因子CXCL10; 和/或生物标志物。 本教导还涉及在临床认可的症状之前检测和监测肺移植排斥发作的方法，治疗决定和试剂盒。

公开(公告)号：WO2012003412A2

公开(公告)日：2012-01-05

申请号：PCT/US2011042723

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORP ,  BALACHANDRAN PRIYA ,  CUMMINGS CRAIG ,  PETRAUSKENE OLGA ,  PAWAR HEMANT ,  TEBBS ROBERT ,  FURTADO MANOHAR

Inventor： BALACHANDRAN PRIYA ,  CUMMINGS CRAIG ,  PETRAUSKENE OLGA ,  PAWAR HEMANT ,  TEBBS ROBERT ,  FURTADO MANOHAR

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/158 ,  Y02A50/451

Abstract: The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.

Abstract translation: 本申请描述了使用可诱导RNA快速检测和鉴定各种微生物的组合物，方法和试剂盒。 描述了通过使用诱导剂在靶基因诱导后检测靶基因的诱导型RNA的表达来快速检测微生物的方法。 一些实施方案描述了用于快速检测微生物的方法和工作流程，例如但不限于沙门氏菌属，李斯特氏菌属（Listeria spp）。 和弧菌属（Vibrio spp。 组合物和试剂盒包含对对一种或多种RNA诱导剂有响应的微生物（或基因片段）基因的引物扩增具有杂交特异性的引物核酸序列。 在一些实施方案中，试剂盒和组合物还包含对对RNA诱导剂或其片段有响应的基因具有杂交特异性的探针核酸序列。

公开(公告)号：WO2012003380A2

公开(公告)日：2012-01-05

申请号：PCT/US2011042683

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORP ,  LEVINE PETER

Inventor： LEVINE PETER

IPC: F24D19/02

CPC classification number: G01N27/4148 ,  C12Q1/6869 ,  G01J1/46 ,  G01N27/4143 ,  G01N27/4145 ,  G01N33/00 ,  G01R29/26 ,  H01L27/14609 ,  H01L27/14632 ,  H01L27/14643 ,  H01L29/66 ,  H01L2924/0002 ,  H04N5/374 ,  Y10T436/25875 ,  H01L2924/00

Abstract: The described embodiments may provide a chemical detection circuit with an improved signal-to-noise ration. The chemical detection circuit may include a current source, a chemical detection pixel, an amplifier and a capacitor. The chemical detection pixel may comprise a chemical-sensitive transistor that may have a first and second terminals and a row-select switch coupled between the current source and chemically- sensitive transistor. The amplifier may have a first input and a second input, with the first input coupled to an output of the chemically-sensitive transistor via a switch and the second input coupled to an offset voltage line. The capacitor may be coupled between an output of the amplifier and the first input of the amplifier. The capacitor and amplifier may form an integrator and may be shared by a column of chemical detection pixels.

Abstract translation: 所描述的实施例可以提供具有改进的信噪比的化学检测电路。 化学检测电路可以包括电流源，化学检测像素，放大器和电容器。 化学检测像素可以包括可具有第一和第二端子的化学敏感晶体管和耦合在电流源和化学敏感晶体管之间的行选择开关。 放大器可以具有第一输入和第二输入，其中第一输入经由开关耦合到化学敏感晶体管的输出，并且第二输入耦合到偏移电压线。 电容器可以耦合在放大器的输出端和放大器的第一输入端之间。 电容器和放大器可以形成积分器并且可以被一列化学检测像素共享。

公开(公告)号：WO2011139920A2

公开(公告)日：2011-11-10

申请号：PCT/US2011034675

申请日：2011-04-29

Applicant: LIFE TECHNOLOGIES CORP ,  CHEN CAIFU

Inventor： CHEN CAIFU

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6851 ,  C12Q2523/125 ,  C12Q2561/113

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating between different methylated and/or unmethylated nucleic acid loci. In certain embodiments, the inventions provides for detecting or quantitating undifferentiated embryonic stem cells in a population of differentiated cells. The invention is also useful for discriminating between fetal versus maternal cells, or healthy versus infected cells, or normal versus cancerous cells, or detecting reduction in viral load or measuring therapeutic efficiency in a patient, and more.

Abstract translation: 在一些实施方案中，本发明一般涉及用于区分不同甲基化和/或未甲基化核酸基因座的组合物，方法和试剂盒。 在某些实施方案中，本发明提供了在分化细胞群中检测或定量未分化的胚胎干细胞。 本发明还可用于区分胎儿与母体细胞或健康对感染细胞或正常与癌细胞，或检测患者病毒载量的降低或测量患者的治疗效果等。

公开(公告)号：WO2011066467A3

公开(公告)日：2011-10-27

申请号：PCT/US2010058111

申请日：2010-11-24

Applicant: LIFE TECHNOLOGIES CORP ,  GREEN ROBERT ,  MULERO JULIO ,  HENNESSY LORI ,  LAGACE ROBERT ,  CHANG CHIEN-WEI

Inventor： GREEN ROBERT ,  MULERO JULIO ,  HENNESSY LORI ,  LAGACE ROBERT ,  CHANG CHIEN-WEI

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q2600/156 ,  C12Q2600/166

Abstract: Disclosed are rare short tandem repeat (STR) alleles within the D10S1248 and D12S391 loci in humans. Provided are representative allelic ladders for each locus, methods and assays using these alleles and kits containing allelic ladders comprising these alleles for accurate genotyping and identification of a wide range of individuals.

Abstract translation: 在人类D10S1248和D12S391位点内公开了罕见的短串联重复（STR）等位基因。 提供了每个基因座的代表性等位基因梯，使用这些等位基因的试剂盒和方法以及含有包含这些等位基因的等位基因梯的试剂盒，用于精确基因分型和鉴定各种个体。

公开(公告)号：WO2011127454A2

公开(公告)日：2011-10-13

申请号：PCT/US2011031855

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP ,  JANAWAY GORDON ,  CHAN EVELYN WING-SIM

Inventor： JANAWAY GORDON ,  CHAN EVELYN WING-SIM

IPC: G06F19/00 ,  G06F3/00

CPC classification number: G06F19/18 ,  G06F3/0482 ,  G06F3/0485 ,  G06F19/26

Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.

Abstract translation: 系统和方法用于显示从qPCR仪器获得的数据。 用第一标记探针和第二标记探针探测两个或更多个样品中的每一个。 从qPCR仪器以第一周期数接收第一数据集，其中包括针对每个样本的第一标记探针强度和第二标记探针强度。 第二数据集以第二周期数被接收，其包括针对每个样本的第一标记探针强度和第二标记探针强度。 使用第一数据集创建第一标记探针强度作为第二标记探针强度的函数的第一图。 使用第二数据集创建第二标记探针强度作为第二标记探针强度的函数的第二图。 显示第一个绘图和第二个绘图以响应用户定义的输入，以提供对基因分型数据的动态和实时分析。

公开(公告)号：WO2011068764A3

公开(公告)日：2011-10-13

申请号：PCT/US2010058242

申请日：2010-11-29

Applicant: LIFE TECHNOLOGIES CORP ,  KADUCHAK GREGORY ,  WARD MICHAEL DENNIS ,  BRADFORD JOLENE ANN ,  PARKER ANDREW THOMAS GEORGE

Inventor： KADUCHAK GREGORY ,  WARD MICHAEL DENNIS ,  BRADFORD JOLENE ANN ,  PARKER ANDREW THOMAS GEORGE

IPC: G01N35/08 ,  G01N33/483

CPC classification number: G01N21/453 ,  G01N15/1404 ,  G01N2015/142

Abstract: A flow cytometer includes a capillary having a sample channel; at least one vibration producing transducer coupled to the capillary, the at least one vibration producing transducer being configured to produce an acoustic signal inducing acoustic radiation pressure within the sample channel to acoustically concentrate particles flowing within a fluid sample stream in the sample channel; and an interrogation source having a violet laser and a blue laser, the violet and blue lasers being configured to interact with at least some of the acoustically concentrated particles to produce an output signal.

Abstract translation: 流式细胞仪包括具有样品通道的毛细管; 至少一个振动产生换能器，其耦合到所述毛细管，所述至少一个振动产生换能器被配置为产生引起所述样本通道内的声辐射压力的声信号，以声学地集中在所述样本通道中的流体样本流内流动的粒子; 以及具有紫色激光器和蓝色激光器的询问源，紫色和蓝色激光器被配置为与至少一些声学上集中的粒子相互作用以产生输出信号。

公开(公告)号：WO2011116120A2

公开(公告)日：2011-09-22

申请号：PCT/US2011028704

申请日：2011-03-16

Applicant: LIFE TECHNOLOGIES CORP ,  LAFFERTY W MICHAEL ,  BEECHEM JOSEPH ,  SUN HONGYE ,  METZKER MICHAEL

Inventor： LAFFERTY W MICHAEL ,  BEECHEM JOSEPH ,  SUN HONGYE ,  METZKER MICHAEL

IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/50

CPC classification number: C12Q1/6874 ,  C12Q2523/319 ,  C12Q2525/186 ,  C12Q2565/513

Abstract: A method of sequencing a plurality of template nucleotide sequences includes immobilizing the plurality of template nucleotide sequences on a substrate. A first subset of the plurality of template nucleotide sequences is immobilized in a first field of view and a second subset of the plurality of template nucleotide sequences is immobilized in a second field of view. The first and second subsets are hybridized to a caged primer. The caged primer includes a caging group. The method further includes lysing the caging group from the caged primer in the first field of view and observing the first field of view to detect sequencing of the first subset of the plurality of template nucleotide sequences.

Abstract translation: 对多个模板核苷酸序列进行测序的方法包括将多个模板核苷酸序列固定在底物上。 多个模板核苷酸序列的第一子集被固定在第一视场中，并且多个模板核苷酸序列的第二子集被固定在第二视野中。 第一和第二子集与笼式引物杂交。 笼状引物包括笼养组。 该方法还包括在第一视场中从笼式引物裂解笼基组，并观察第一视野以检测多个模板核苷酸序列的第一子集的测序。

公开(公告)号：WO2011028834A3

公开(公告)日：2011-07-07

申请号：PCT/US2010047573

申请日：2010-09-01

Applicant: LIFE TECHNOLOGIES CORP ,  WOJTOWICZ JANUSZ ,  DAHLHOFF GEOFFREY ,  GRUNEWALD DOUGLAS W ,  CONNER THOMAS A

Inventor： WOJTOWICZ JANUSZ ,  DAHLHOFF GEOFFREY ,  GRUNEWALD DOUGLAS W ,  CONNER THOMAS A

IPC: G06F1/20 ,  H05K7/20

CPC classification number: H01L21/4882 ,  F28D2021/0029 ,  H01L23/467 ,  H01L2924/0002 ,  H01L2924/00

Abstract: The present teachings disclose various embodiments of a thermal block assembly having low thermal non-uniformity throughout the thermal block assembly. Accordingly, various embodiments of thermal block assemblies having such low thermal non-uniformity provide for desired performance of bioanalysis instrumentation utilizing such thermal block assemblies.

Abstract translation: 本教导公开了整个热块组件中具有低热不均匀性的热块组件的各种实施例。 因此，具有这种低热不均匀性的热块组件的各种实施例提供了利用这种热块组件的生物分析仪器的期望性能。

公开(公告)号：WO2011072078A2

公开(公告)日：2011-06-16

申请号：PCT/US2010059573

申请日：2010-12-08

Applicant: LIFE TECHNOLOGIES CORP ,  ADAIR NICHOLAS ,  CRESPO JOSELITO ,  RUSSELL PAUL ,  PERRY ALLEN

Inventor： ADAIR NICHOLAS ,  CRESPO JOSELITO ,  RUSSELL PAUL ,  PERRY ALLEN

IPC: B65D75/34 ,  B65D77/26 ,  B65D85/30 ,  G01N1/00

CPC classification number: B65D25/105 ,  B01L9/06 ,  B01L2200/025 ,  B01L2200/185 ,  B01L2300/021 ,  B65D81/025

Abstract: A packaging system for transporting vials containing biological samples may comprise a first tray defining at least one first tray cavity; and a second tray defining at least one second tray cavity and configured to mate with the first tray. The packaging system may further comprise at least one first tray cavity and at least one second tray cavity, wherein the at least one first tray cavity and the at least one second tray cavity are configured to securely hold respective vials for transport, and to restrain caps on the respective vials during transport, wherein the at least one first tray cavity and the at least one second tray cavity oppose each other when the first tray and the second tray are mated together. The packaging system may also be configured to permit barcode scanning of vials held within the first tray cavity and the second tray ca

Abstract translation: 用于运送含有生物样本的小瓶的包装系统可以包括限定至少一个第一托盘腔的第一托盘; 以及限定至少一个第二托盘空腔并且构造成与第一托盘配合的第二托盘。 包装系统可以进一步包括至少一个第一托盘空腔和至少一个第二托盘空腔，其中所述至少一个第一托盘空腔和所述至少一个第二托盘空腔被配置为牢固地保持用于运输的相应小瓶，并且限制盖帽 在运输期间在相应的小瓶上，其中当第一托盘和第二托盘配合在一起时，至少一个第一托盘空腔和至少一个第二托盘空腔彼此相对。 包装系统还可以被配置成允许保持在第一托盘腔和第二托盘ca内的小瓶的条形码扫描