公开(公告)号：WO2012018964A1

公开(公告)日：2012-02-09

申请号：PCT/US2011/046512

申请日：2011-08-03

Applicant: LIFE TECHNOLOGIES CORPORATION ,  TEBBS, Robert ,  CUMMINGS, Craig ,  NUNEZ, Arlene ,  BALACHANDRAN, Priya ,  FATEMI, Payman ,  PETRAUSKENE, Olga

Inventor： TEBBS, Robert ,  CUMMINGS, Craig ,  NUNEZ, Arlene ,  BALACHANDRAN, Priya ,  FATEMI, Payman ,  PETRAUSKENE, Olga

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2561/113 ,  C12Q2600/156 ,  Y02A50/451

Abstract: Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of Salmonella enterica , subspecies enterica, serovar Enteritidis ( S . Enteritidis) in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect S. Enteritidis without cross-reacting with S. Paratyphi C or other closely related Salmonella species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan® assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.

Abstract translation: 本公开的实施方案涉及分离的核酸序列的组合物，方法，工作流程和其用于检测肠炎沙门氏菌，肠亚科，血清型肠炎沙丁杆菌（肠炎沙丁杆菌）的样品中尤其是食品（鸡蛋，家禽） 或环境样品（包括家禽相关环境样品）。 本公开的实施方案也可用于差异检测肠杆菌，而不与副伤寒杆菌C或其他密切相关的沙门氏菌属物种交叉反应。 在一些实施方案中，本公开的方法和试剂盒可以包含TaqMan测定。 样品富集后，检测方法可在约3小时内完成。

公开(公告)号：WO2012003412A2

公开(公告)日：2012-01-05

申请号：PCT/US2011042723

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORP ,  BALACHANDRAN PRIYA ,  CUMMINGS CRAIG ,  PETRAUSKENE OLGA ,  PAWAR HEMANT ,  TEBBS ROBERT ,  FURTADO MANOHAR

Inventor： BALACHANDRAN PRIYA ,  CUMMINGS CRAIG ,  PETRAUSKENE OLGA ,  PAWAR HEMANT ,  TEBBS ROBERT ,  FURTADO MANOHAR

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/158 ,  Y02A50/451

Abstract: The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.

Abstract translation: 本申请描述了使用可诱导RNA快速检测和鉴定各种微生物的组合物，方法和试剂盒。 描述了通过使用诱导剂在靶基因诱导后检测靶基因的诱导型RNA的表达来快速检测微生物的方法。 一些实施方案描述了用于快速检测微生物的方法和工作流程，例如但不限于沙门氏菌属，李斯特氏菌属（Listeria spp）。 和弧菌属（Vibrio spp。 组合物和试剂盒包含对对一种或多种RNA诱导剂有响应的微生物（或基因片段）基因的引物扩增具有杂交特异性的引物核酸序列。 在一些实施方案中，试剂盒和组合物还包含对对RNA诱导剂或其片段有响应的基因具有杂交特异性的探针核酸序列。

公开(公告)号：WO2012003409A1

公开(公告)日：2012-01-05

申请号：PCT/US2011/042720

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  PETRAUSKENE, Olga ,  CUMMINGS, Craig ,  VATTA, Paolo ,  TEBBS, Robert ,  BALACHANDRAN, Priya ,  ZODER, Patrick ,  WONG, Lily

Inventor： PETRAUSKENE, Olga ,  CUMMINGS, Craig ,  VATTA, Paolo ,  TEBBS, Robert ,  BALACHANDRAN, Priya ,  ZODER, Patrick ,  WONG, Lily

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp . Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi . Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

Abstract translation: 本公开的实施方案涉及分离的核酸序列，其使用方法和用于检测样品中的几种利斯特氏菌物种的工作流程，特别是在食品或环境样品中。 本公开的实施方案还可以用于彼此检测李斯特氏菌的一种或多种物种或菌株，例如，可以独立于其他李斯特菌属物种检测L.crilli。 一些实施方案还描述了可以检测单核细胞增生李斯特氏菌（L. innocua），威尔逊氏乳杆菌（L.Welshimeri），塞贝尔氏球菌（L.ceelgeri），马氏体（L.marthii）（以前称为incertae-sedis），伊马诺病毒L. 还描述了用于检测李斯特菌的试剂盒。 在一些实施方案中，本公开的方法和试剂盒可以包括TAQMAN测定。 在一些实施方案中，0.2-2cfu的李斯特菌属 在24-28小时浓缩期后使用组合物，方法和试剂盒检测。

公开(公告)号：EP2588628A1

公开(公告)日：2013-05-08

申请号：EP11732541.5

申请日：2011-06-30

Applicant: Life Technologies Corporation ,  Petrauskene, Olga ,  Cummings, Craig ,  Vatta, Paolo ,  Tebbs, Robert ,  Balachandran, Priya ,  Zoder, Patrick ,  Wong, Lily

Inventor： PETRAUSKENE, Olga ,  CUMMINGS, Craig ,  VATTA, Paolo ,  TEBBS, Robert ,  BALACHANDRAN, Priya ,  ZODER, Patrick ,  WONG, Lily

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several
Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of
Listeria from each other, for example
L. grayi may be detected independently of other
Listeria spp . Some embodiments also describe a duplexed assay that can detect
L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and
L. grayi . Kits for detection of
Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of
Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

公开(公告)号：WO2013029021A1

公开(公告)日：2013-02-28

申请号：PCT/US2012/052383

申请日：2012-08-24

Applicant: LIFE TECHNOLOGIES CORPORATION ,  TEBBS, Robert ,  CUMMINGS, Craig ,  BRZOSKA, Pius ,  MATHENY, Sharon ,  O'CONNELL, Catherine ,  FURTADO, Manohar ,  FANG, Rixun ,  PETRAUSKENE, Olga ,  LIEW, Sueh-Ning ,  SCHUMAKER, Michael

Inventor： TEBBS, Robert ,  CUMMINGS, Craig ,  BRZOSKA, Pius ,  MATHENY, Sharon ,  O'CONNELL, Catherine ,  FURTADO, Manohar ,  FANG, Rixun ,  PETRAUSKENE, Olga ,  LIEW, Sueh-Ning ,  SCHUMAKER, Michael

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/689 ,  C12Q2600/16

Abstract: The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin ( stx ) or an eae . In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.

Abstract translation: 本教导描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子（例如志贺毒素（stx）或eae）的一种或多种微生物。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体菌株和血清型。 一些实施方案描述了用于检测STEC微生物的组合物，方法和试剂盒。 还描述了用于多个微生物检测和鉴定的工作流程。

公开(公告)号：WO2012003412A3

公开(公告)日：2012-01-05

申请号：PCT/US2011/042723

申请日：2011-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BALACHANDRAN, Priya ,  CUMMINGS, Craig ,  PETRAUSKENE, Olga ,  PAWAR, Hemant ,  TEBBS, Robert ,  FURTADO, Manohar

Inventor： BALACHANDRAN, Priya ,  CUMMINGS, Craig ,  PETRAUSKENE, Olga ,  PAWAR, Hemant ,  TEBBS, Robert ,  FURTADO, Manohar

IPC: C12Q1/68

Abstract: The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.

公开(公告)号：WO2011053871A3

公开(公告)日：2011-09-29

申请号：PCT/US2010054867

申请日：2010-10-29

Applicant: LIFE TECHNOLOGIES CORP ,  PETRAUSKENE OLGA ,  BRZOSKA PIUS ,  BIT SOMAYA ,  LIU JEN-KUEI ,  TEBBS ROBERT ,  FURTADO MANOHAR

Inventor： PETRAUSKENE OLGA ,  BRZOSKA PIUS ,  BIT SOMAYA ,  LIU JEN-KUEI ,  TEBBS ROBERT ,  FURTADO MANOHAR

IPC: C12Q1/68 ,  C12P19/34 ,  G01N33/48

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

Abstract translation: 公开了一种多引物扩增测定法，用于利用在单个容器中与样品接触的多个寡核苷酸引物检测支原体物种和密切相关物种的检测扩增产物，其中扩增产物的存在表示支原体 在样品中。

公开(公告)号：WO2011053871A2

公开(公告)日：2011-05-05

申请号：PCT/US2010/054867

申请日：2010-10-29

Applicant: LIFE TECHNOLOGIES CORPORATION ,  PETRAUSKENE, Olga ,  BRZOSKA, Pius ,  BIT, Somaya ,  LIU, Jen-Kuei ,  TEBBS, Robert ,  FURTADO, Manohar

Inventor： PETRAUSKENE, Olga ,  BRZOSKA, Pius ,  BIT, Somaya ,  LIU, Jen-Kuei ,  TEBBS, Robert ,  FURTADO, Manohar

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

Abstract translation: 公开了利用与单个容器中的样品接触的多个寡核苷酸引物并检测扩增产物的用于检测支原体物种和紧密相关物种的多引物扩增测定法，方法和试剂盒，其中 扩增产物的存在表明样品中的支原体。

公开(公告)号：EP2748332A1

公开(公告)日：2014-07-02

申请号：EP12761842.9

申请日：2012-08-24

Applicant: Life Technologies Corporation ,  Tebbs, Robert ,  Cummings, Craig ,  Brzoska, Pius ,  Matheny, Sharon ,  O'Connell, Catherine ,  Furtado, Manohar ,  Fang, Rixun ,  Petrauskene, Olga ,  Liew, Sueh-Ning ,  Schumaker, Michael

Inventor： TEBBS, Robert ,  CUMMINGS, Craig ,  BRZOSKA, Pius ,  MATHENY, Sharon ,  O'CONNELL, Catherine ,  FURTADO, Manohar ,  FANG, Rixun ,  PETRAUSKENE, Olga ,  LIEW, Sueh-Ning ,  SCHUMAKER, Michael

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/689 ,  C12Q2600/16

Abstract: The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin (stx) or an eae. In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.

Abstract translation: 本教导描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子（例如志贺毒素（stx）或eae）的一种或多种微生物。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体菌株和血清型。 一些实施方案描述了用于检测STEC微生物的组合物，方法和试剂盒。 还描述了用于多个微生物检测和识别的工作流程。