公开(公告)号：KR1020010004480A

公开(公告)日：2001-01-15

申请号：KR1019990025161

申请日：1999-06-29

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  권병준 ,  우승룡 ,  김종만 ,  조준형

IPC: C12Q1/70

Abstract: PURPOSE: A method for diagnosing Porcine epidemic diarrhea virus(PEDV) and a diagnosis kit are provided, thereby PEDV can be rapidly and simply detected with lower cost. CONSTITUTION: The method for diagnosing Porcine epidemic diarrhea virus(PEDV) comprises the steps of: injection the antigen of Porcine epidemic diarrhea virus(PEDV) into chickens; separating vitellus from eggs of immunized chickens; producing monoclonal antibody IgG2a from hybridoma PEDV 41-4(KCLRF-BP-00023) using the vitellus; attaching the monoclonal antibody of Porcine epidemic diarrhea virus(PEDV) into a plate; reacting with testing samples; and reacting with the specific antigen, in which monoclonal antibody IgG2a can reacts with 30 to 33 kDa proteins of PEDV. The PEDV diagnosis kit contains the monoclonal antibody IgG2a and can diagnose PEDV by detecting the change of color.

Abstract translation: 目的：提供一种诊断猪流行性腹泻病毒（PEDV）和诊断试剂盒的方法，从而以较低的成本快速，简便地检测PEDV。 构成：猪流行性腹泻病毒（PEDV）的诊断方法包括：将猪流行性腹泻病毒（PEDV）的抗原注射入鸡中; 将卵白蛋白与免疫鸡的卵分离; 使用卵白素从杂交瘤PEDV 41-4（KCLRF-BP-00023）产生单克隆抗体IgG2a; 将猪流行性腹泻病毒（PEDV）的单克隆抗体连接到板中; 与测试样品反应; 并与特异性抗原反应，其中单克隆抗体IgG2a可与30至33kDa的PEDV蛋白质反应。 PEDV诊断试剂盒含有单克隆抗体IgG2a，可通过检测颜色变化来诊断PEDV。