Abstract:
A transformed recombinant strain by transforming a tliDEF ABC transporter extracted from a Pseudomonas fluorescens sp. strain and a DNA encoding a lipase(tliA) into a Serratia marcescens sp. strain using a plasmid vector is provided to mass-produce the lipase with higher activity than a conventional strain and provide the lipase not in an activated form using an ABC type protein secretion transporter, thereby obtaining the lipase with high yield economicallyh without crushing the original strain. A recombinant plasmid vector is characterized in that an ABC type protein secretion transporter derived from Gram negative bacteria and a gene encoding lipase are introduced into a plasmid vector. Serratia marcescens sp. recombinant bacteria are characterized in that an original Serratia marcescens sp. strain is transformed by the recombinant plasmid vector. A method for producing lipase comprises the steps of: (a) preparing a recombinant plasmid vector where a tliDEF ABC transporter derived from a Pseudomonas fluorescens sp. strain and a DNA framgent encoding the lipase are introduced; (b) preparing the transformed Serratia marcescens sp. recombinant strain by transforming the original Serratia marcescens sp. strain using the recombinant plasmid vector; (c) culturing the transformed Serratia marcescens sp. recombinant strain in a culture medium to induce extracellular secretion of the lipase; and (d) recovering the lipase from a culture broth obtained from the step(c).
Abstract:
A method for preparing a phenolic polymer by using coprinus cinereus-derived peroxidase is provided to obtain a phenolic polymer for photoresist having a controlled polymerization degree, molecular weight distribution and hydroxyl content under a mild condition, thereby producing photoresist with high resolution. A method for preparing a phenolic polymer comprises the steps of: dissolving a phenolic monomer represented by the following formula 1 and coprinus cinereus-derived peroxidase in a polar solvent to form a mixed solution; supplying hydrogen peroxide constantly to the mixed solution at a reaction temperature of 5-20 deg.C for 2-24 hours to perform polymerization. In formula 1, X is any one selected from nil, CH2, C(CH3)2, O and S.
Abstract:
본 발명은 페노티아진계 전달물질을 이용한 페놀계 고분자의 제조방법에 관한 것으로서, 더욱 상세하게는 퍼록시데이즈 생촉매와 산화제 존재 하에서 페놀계 단량체를 중합함에 있어 페노티아진계 전달물질을 추가로 사용하여 퍼록시데이즈의 효소 반응성을 개선시키는 페놀계 고분자의 제조방법에 관한 것이다. 본 발명의 중합방법으로 제조된 페놀계 고분자는 곁 사슬로 결합된 불포화 탄화수소기가 그대로 보존되어 있어 라디칼 경화반응이 용이하므로 경화수지로서 유용하게 사용될 수 있고, 또한 상기 경화수지가 포함된 도료가 코팅된 도막은 항산화능이 우수하고, 도막의 표면 에너지가 작아서 물리적인 해양생물의 부착을 저해하며, 방오작용기의 소모가 발생되지 않아 지속적으로 내구성을 발현하게 된다. 페노티아진, 전달물질, 페놀계 고분자, 퍼록시데이즈, 생촉매, 산화제, 페놀계 단량체
Abstract:
PURPOSE: A coating resin latex composition for transferring is provided, to improve the storage stability, the durability and the adhesion and to reduce the depth difference between upper and lower faces the bubble generation. CONSTITUTION: The coating resin latex composition comprises 100 parts by weight of an epoxy resin; 20-80 parts by weight of a curing agent; 0.05-5 parts by weight of a curing promoter; and an organic solvent, wherein the curing promoter is a mixture of triethanolamine and one or more compounds of diethanolamines by the ratio of 1 : 0.8-3.5. The diethanolamines are selected from diethanolamine, methyldiethanolamine, ethyldiethanolamine, n-butyldiethanolamine and t-butyldiethanolamine. The epoxy resin is preferably prepared by glycidylating bisphenol, phenol, phenol-formaldehyde or dicarboxylic acid and the equivalence of epoxy is 180-3,300.
Abstract:
The method comprises graft-reacting a crystalline polypropylene resin with a radical-reactive dicarboxilic acid or its acid anhydride in the presence of a radical initiator at 90-140 deg.C. A thiol-containing cpd. of formula (I) is added in the reaction mixture. In the formula (I), R1 and R2 are each H, C1-18 alkyl, alkenyl, vinylbenzyl, benzyl or alkyl (C1-18) benzyl. The amount of the thiol-contg. cpd. is 0.1-10.0 wt. parts to the crystalline polypropylene resin. The graft reaction is proceeded in a solution state.
Abstract:
본 발명은 도장시 전처리 공정없이 직접 도장이 가능한 폴리올레핀계 수지 조성물에 관한 것이다. 본 발명의 직접 도장이 가능한 폴리올레핀계 수지 조성물을 폴리올레핀계 수지 30∼90중량부, 폴리올레핀계 고무 10∼70 중량부, 적어도 2개 이상의 수산기를 함유하는 올레핀계 올리고머 0.1∼30중량부, 에틸렌-아크릴산메틸 공중합체 0.1∼30 중량부와 무기 충전재 0.1∼40중량부로 이루어진 것을 특징으로 한다. 본 발명에 따르면 종래의 도장성 부여 방법에 비하여 플라즈마, 프라이머 등의 전처리 없이 직접 도장이 가능하며, 또한 도막 밀착성이 우수한 폴리올레핀계 수지 조성물로 자동차의 범퍼, 인스트루먼트 패널 등 도장을 필요로 하는 각종 산업 제품에 널리 사용될 수 있다.
Abstract:
본발명은재조합플라스미드벡터, 재조합플라스미드벡터로형질전환된재조합균주및 이를이용한목적단백질생산방법에관한것이다. 본발명을통해 ABC 트랜스포터를이용하여목적단백질을효율적으로수득할수 있으며, 원균주의체외로목적단백질이배출되므로별도로원균주를분쇄하지않고목적단백질을수득할수 있어, 매우경제적이며수득률도높아목적단백질의대량생산에유용하게사용될수 있다.