公开(公告)号：KR1020140065658A

公开(公告)日：2014-05-30

申请号：KR1020120131153

申请日：2012-11-19

Applicant: 대한민국(농촌진흥청장) ,  차의과학대학교 산학협력단

Inventor： 박윤제 ,  서은정 ,  임현섭 ,  한상익 ,  서우덕 ,  나지은 ,  장기창 ,  오성환 ,  최경진 ,  김병주 ,  강현중 ,  이종희 ,  남민희

IPC: C12N1/14 ,  C12M1/10 ,  C12M3/00

Abstract: The present invention relates to a method for promoting the production of Monacolin K in red yeast rice, and a device for rotating and culturing the same. The method comprises a method for culturing red yeast rice which is contained in a culturing container. The culturing container is horizontally laid between two support rods which can be rotated in order to come in contact with the support rods. The culturing container is rotated at a predetermined speed using electric power of the support rods so that the culturing container which comes in contact with the surface of a roller is rotated together and the red yeast rice is cultured. When a large amount of red yeast rice is cultured using the device of the present invention, the production amount of Monacolin K can be more increased than when red yeast rice is cultured through existing culturing methods. Moreover, large-scale contamination which is generated when the red yeast rice is cultured in a rotating culturing container can be prevented and an automatic agitating device can be utilized so that uniform a solid fermented material can be obtained. The device can be mechanically operated so that a person does not have to perform agitation for culturing in bags by himself/herself and required man power can be reduced.

Abstract translation: 本发明涉及一种用于促进红曲米中莫纳可林K的生产的方法及其旋转和培养装置。 该方法包括培养容器中含有的红曲米的培养方法。 培养容器水平放置在两个支撑杆之间，可以旋转以便与支撑杆接触。 使用支撑杆的电力使培养容器以预定速度旋转，从而使与辊的表面接触的培养容器一起旋转，并培养红色酵母米。 当使用本发明的装置培养大量的红酵母大米时，与通过现有培养方法培养红酵母米时相比，莫纳可林K的产生量可以增加。 此外，可以防止在旋转培养容器中培养红酵母米时产生的大规模污染物，并且可以利用自动搅拌装置，从而可以获得均匀的固体发酵材料。 该装置可以机械地操作，使得人们不必为自己在袋子中进行搅拌，并且可以减少所需的人力。

公开(公告)号：KR1020140046570A

公开(公告)日：2014-04-21

申请号：KR1020120110742

申请日：2012-10-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 황운하 ,  김상민 ,  박수권 ,  권택민 ,  박동수 ,  이종희 ,  조준현 ,  신동진 ,  김상열 ,  이지윤 ,  한상익 ,  여운상 ,  송유천 ,  강항원 ,  남민희

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2600/13

Abstract: The present invention provides: a primer set for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; a kit for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; and a method of differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors by performing an amplification reaction using the primer set. By using the primer set of the present invention, a user can differentiate black rice in a seedling condition compared to an existing sorting method of confirming the color of seed coats after the vegetative growth, discharging water, and grain filling; and the homomorphy and heteromorphy of characteristics can be possible, thereby improving the breeding efficiency utilizing the juvenile selection of black rice and minimum resources. [Reference numerals] (AA) Boseokheugchal(black rice); (BB) No.244 of Miryang(white rice); (CC) Approximately 450 bp; (DD) Approximately 215 bp

Abstract translation: 本发明提供了一种用于分化含有花青素和其他糙米种衣色的黑米的种皮颜色的引物组; 一种用于区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的试剂盒; 以及通过使用引物组进行扩增反应来区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的方法。 通过使用本发明的引物组，与用于确认营养生长后的种皮颜色的现有分类方法相比，使用者能够在苗期条件下区分黑米，排出水和灌浆; 特色的同性异体和异质性是可能的，从而通过青少年选择黑米和最低限度的资源提高育种效率。 （AA）Boseokheugchal（黑米）; （BB）米阳244号（白米）; （CC）约450bp; （DD）约215bp

公开(公告)号：KR101322345B1

公开(公告)日：2013-10-28

申请号：KR1020110030389

申请日：2011-04-01

Applicant: 대한민국(농촌진흥청장)

Inventor： 박수권 ,  이종희 ,  곽도연 ,  박동수 ,  조준현 ,  이지윤 ,  황운하 ,  김상열 ,  김상민 ,  권택민 ,  오성환 ,  정국현 ,  여운상 ,  송유천 ,  강항원

IPC: C12Q1/68 ,  C12N15/11 ,  C12N15/29

Abstract: 본 발명은 RM516, RM6082, RM18182, RM18184, RM18185, RM5844 및 RM7409로 구성되는 군으로부터 선택된 하나 이상의 올리고뉴클레오티드 또는 이의 단편의 존재여부를 검출하는 프라이머를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물, 구체적으로는, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물에 관한 것이다. 또한, 본 발명은 벼 시료에서 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 이용하여 증폭 반응을 수행하여 표적 서열을 증폭하는 단계; 및 상기 증폭 산물을 검출하는 단계를 포함하는, 끝동매미충 저항성 벼 품종 선별 방법 및 벼 선별 키트에 관한 것이다. 본 발명에 따른 끝동매미충 저항성 개체를 선발하는 방법을 이용하면, 끝동매미충 저항성 유전자의 위치와 아주 밀접하게 연관된 마커를 이용하여 효율적으로 끝동매미충 저항성 및 감수성 개체를 구분할 수 있다.

公开(公告)号：KR1020130091434A

公开(公告)日：2013-08-19

申请号：KR1020120012710

申请日：2012-02-08

Applicant: 대한민국(농촌진흥청장)

Inventor： 박동수 ,  박수권 ,  황운하 ,  이종희 ,  김상민 ,  조준현 ,  곽도연 ,  윤영남 ,  여운상 ,  송유천 ,  강항원 ,  권택민

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q1/6844 ,  C12Q2600/13 ,  C12Q2600/158

Abstract: PURPOSE: A method of selecting a breed that is resistive to a rice stripe disease is provided to efficiently separate a breed that is resistive and sensitive with respect to the rice stripe disease by using a marker, which is closely related with a location of a rice stripe disease-resistant gene. CONSTITUTION: A pair of primers for selecting a breed of rice resistive against a rice stripe disease is selected from the group consisting of a primer pair represented by SEQ ID NO: 1 and 2; a primer pair represented by SEQ IE NO: 3 and 4; a primer pair represented by SEQ IE NO: 5 and 6; a primer pair represented by SEQ IE NO: 7 and 8; a primer pair represented by SEQ IE NO: 9 and 10; a primer pair represented by SEQ IE NO: 11 and 12; a primer pair represented by SEQ IE NO: 13 and 14; a primer pair represented by SEQ IE NO: 15 and 16; a primer pair represented by SEQ IE NO: 17 and 18; a primer pair represented by SEQ IE NO: 19 and 20; and a primer pair represented by SEQ IE NO: 21 and 22. A method of selecting a rice stripe disease-resistant breed comprises the steps of: (i) isolating DNA from a rice sample; (ii) amplifying a target sequence by performing an amplification reaction by having the isolated DNA as a template and using the at least one primer pair selected from above; and (iii) detecting the amplification product.

Abstract translation: 目的：提供一种选择抗稻瘟病抗性品种的方法，通过使用与稻米位置密切相关的标记，有效地分离出与水稻条纹病有关的抗性和敏感性品种 条纹抗病基因。 构成：从由SEQ ID NO：1和2表示的引物对组成的组中选择一对用于选择抗稻瘟病抗性的水稻品种的引物; 由SEQ ID NO：3和4表示的引物对; 由SEQ ID NO：5和6表示的引物对; 由SEQ ID NO：7和8表示的引物对; 由SEQ ID NO：9和10表示的引物对; 由SEQ ID NO：11和12表示的引物对; 由SEQ ID NO：13和14表示的引物对; 由SEQ ID NO：15和16表示的引物对; 由SEQ ID NO：17和18表示的引物对; 由SEQ ID NO：19和20表示的引物对; 和由SEQ ID NO：21和22表示的引物对。选择水稻条纹抗病品种的方法包括以下步骤：（i）从稻样品中分离DNA; （ii）通过使分离的DNA作为模板并使用选自上述的至少一种引物对进行扩增反应来扩增靶序列; 和（iii）检测扩增产物。

公开(公告)号：KR100393380B1

公开(公告)日：2003-07-31

申请号：KR1020000050892

申请日：2000-08-30

Applicant: 대한민국(농촌진흥청장)

Inventor： 좌재호 ,  임한철 ,  한승갑 ,  이종희 ,  정순경

IPC: C12N1/20

Abstract: PURPOSE: Provided is a novel microorganism which solubilizes fixed phosphates in soil of volcanic lime ash, thereby utilizing phosphates fixed in soil of volcanic lime ash in a tangerine orchard. CONSTITUTION: A novel microorganism strain, Bacillus spharicus PSB-13(KCTC 18005P) is excellent in phosphate solubilization and easily adapts itself to the condition of soil of volcanic lime ash. It shows viability at pH 4-7 and 4-70 deg.C and has an organic acid productability to produce citric acid and oxalic acid. It is also easily mixed with organics and used.

Abstract translation: 目的：提供了一种新的微生物，其将固定的磷酸盐溶解在火山灰灰土中，从而利用在橘园中的火山灰灰土中固定的磷酸盐。 构成：新型微生物菌株Bacillus spharicus PSB-13（KCTC 18005P）具有优异的磷酸盐增溶作用，并易于适应火山灰灰土的条件。 它在pH 4-7和4-70摄氏度显示活力，并具有产生柠檬酸和草酸的有机酸生产能力。 它也很容易与有机物混合使用。