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公开(公告)号：KR1020100051354A
公开(公告)日：2010-05-17
申请号：KR1020080110485
申请日：2008-11-07
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A controlling method of the flowering time of a plant, and a transcription factor controlling the flowering time are provided to enhance the productivity of crops by controlling the growth of the plant. CONSTITUTION: A bZIP(basic leucine zipper domain) transcription factor controls the flowering time of a plant. The transcription factor is originated from an oryza sativa, while including a base sequence with the sequence number 1. A bZIP transcription factor OREB2-D71 includes the base sequence with the sequence number 2, and a transcription activation domain. A bZIP transcription factor protein is formed with the base sequence with the sequence number 3. The control of the flowering time of the plant is performed by over-expressing the OREB2-D71 by delaying the flowering time.
Abstract translation: 目的：提供植物开花时间的控制方法和控制开花时间的转录因子，以通过控制植物的生长来提高作物的生产力。 构成：bZIP（碱性亮氨酸拉链结构域）转录因子控制植物的开花时间。 转录因子来源于稻谷，同时包含序列号为1的碱基序列.BZIP转录因子OREB2-D71包括序列号为2的碱基序列和转录激活结构域。 用序列号3的碱基序列形成bZIP转录因子蛋白质。通过延迟开花时间来过表达OREB2-D71来进行植物开花时间的控制。
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公开(公告)号：KR100925884B1
公开(公告)日：2009-11-09
申请号：KR1020070126691
申请日：2007-12-07
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 BrSRS의 세포내 수준을 조절하여 식물의 형태를 변화시키는 방법에 관한 것으로서, 보다 상세하게는 배추(
Brassica
rapa )에서 분리한 식물체 크기 및 생장발달에 영향을 주는 BrSRS 유전자를 이용하여 식물의 형태를 변화시키는 방법 및 형태가 변화된 형질전환 식물에 관한 것이다. 본 발명은 BrSRS 유전자의 세포내 발현을 조절하여 식물의 형태를 변화시키는 방법을 제공한다. 따라서, 본 발명은 식물체 크기, 잎 모양, 엽색과 종자 크기를 변형하고자 할 경우, 특히 식물 크기를 작게 만들어 관상용 형태를 목적으로 하거나, 잎 모양에 굴곡(wave)형태 또는 잎 모양이 위로 말린 모양을 도입하여 원예적 가치를 높이거나, 또 종자의 크기를 더 크게 하여 수량성을 높이고자 하는 경우 등에 사용할 수 있을 것이다.
BrSRS, 배추, 형질전환체, 애기장대, 생육, 표현형-
Patent Agency Ranking83.发明公开벼에서 분리된 종자 형성 및 생육 관련 유전자, 이유전자를 포함하는 발현벡터, 이 발현 벡터로 형질 전환된형질전환체, 및 이 형질전환체의 제조 방법 无效与ORYZA SATIVA，包含基因的表达载体，由转化体转化的转化体和转化体的制备方法分离的种子发育相关的基因
公开(公告)号：KR1020090034544A
公开(公告)日：2009-04-08
申请号：KR1020070099821
申请日：2007-10-04
Applicant: 대한민국(농촌진흥청장)
Abstract: A gene related to seed development isolated from oryza sativa, an expression vector a transformant and a method for preparation of the transformant are provided to offer plants of which seed size is increased and growth differentiation is controlled effectively. An oryza sativa enhancer of zeste zene related to seed formation and rearing includes a base sequence written as a sequence number 1. A method for preparation of a transformant transformed to the oryza sativa enhancer of zeste zene comprises the following steps of: manufacturing an expression vector including a sequence of the oryza sativa enhancer of zeste DNA and connected to a DNA sequence representing activity of a promoter; introducing the expression vector in a host cell selected from agrobacterium, arabidopsis thaliana and oryza sativa; and selecting the ransformant.
Abstract translation: 提供了从oryza sativa分离的种子发育相关的基因，表达载体a转化体和转化体的制备方法，以提供种子大小增加并且有效控制生长分化的植物。 与种子形成和饲养相关的泽氏zene的维生素增强剂包括编号为序列号1的碱基序列。一种转化为西地那里维生素增强剂的转化体的制备方法，包括以下步骤：制备表达载体 包括丝氨酸脱水叶绿体增强子的序列，并连接到代表启动子活性的DNA序列; 将表达载体引入选自农杆菌，拟南芥和稻谷的宿主细胞中; 并选择变体。
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公开(公告)号：KR1020080018425A
公开(公告)日：2008-02-28
申请号：KR1020060080503
申请日：2006-08-24
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6851 , C12Q1/6844 , C12Q1/6895 , C12Q2537/143 , G01N33/5308
Abstract: A qualitative and quantitative detection method of genetically modified rice is provided to detect specifically the genetically modified rice in currently distributed or imported agricultural products and food by using PCR(polymerase chain reaction) primer and probe and standard plasmid, and select a homozygote line rice. A genetically modified rice is detected qualitatively and quantitatively by detecting a foreign gene introduced into the rice through PCR by using a PCR primer set CytC-F1265 of SEQ ID NO:1 and CytC-R1345 of SEQ ID NO:2, a probe CytC-1292 of SEQ ID NO:3 and a plasmid p0S2, or a PCR primer set GFP-F203 of SEQ ID NO:4 and GFP-R303 of SEQ ID NO:5, a probe GFP-232 of SEQ ID NO:6 and a plasmid p0S2.
Abstract translation: 提供转基因水稻的定性和定量检测方法，通过使用PCR（聚合酶链式反应）引物和探针和标准质粒来特异性检测目前分布或进口农产品和食品中的转基因水稻，并选择纯合水稻。 通过使用SEQ ID NO：1的PCR引物组CytC-F1265和SEQ ID NO：2的CytC-R1345，探针CytC-F1265，通过PCR检测引入水稻的外源基因，定性和定量检测转基因水稻， SEQ ID NO：3的1292和质粒pOS2，或SEQ ID NO：4的GFP引物组GFP-F203和SEQ ID NO：5的GFP-R303，SEQ ID NO：6的探针GFP-232和 质粒p0S2。
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公开(公告)号：KR1020070110969A
公开(公告)日：2007-11-21
申请号：KR1020060043687
申请日：2006-05-16
Applicant: 대한민국(농촌진흥청장)
Abstract: A protein kinase gene is provided to increase dehydration of crop plants through its overexpression, so that the gene is useful for increasing resistance against stresses of monocotyledon and dicotyledon crop plants. A protein kinase capable of inducing dehydration of crop plants has the amino acid sequence of SEQ ID NO:1 having a protein kinase domain in the N-terminal and an activity-regulating domain in the C-terminal, is isolated from the rice and has phosphorylation activity to a bZIP transcription factor of rice ABF family. A protein kinase gene has 95th-1189th nucleotides in the nucleotide sequence of SEQ ID NO:2, wherein the expression of the protein kinase gene is induced by dry stress and salt. The environment resistance of a crop plant is controlled by overexpression or inhibition of the protein kinase gene. A transgenic plant having increased dehydration is produced by transforming a plant with an expression vector containing the protein kinase gene.
Abstract translation: 提供蛋白激酶基因以通过其过表达来增加作物的脱水，使得该基因可用于增加对单子叶植物和双子叶植物作物的抗性的抗性。 能够诱导作物植物脱水的蛋白激酶具有在N末端具有蛋白激酶结构域的C端的氨基酸序列和C-末端的活性调节结构域，并且从水稻中分离出具有 水稻ABF家族的bZIP转录因子的磷酸化活性。 蛋白激酶基因在SEQ ID NO：2的核苷酸序列中具有第95〜第1189位核苷酸，其中蛋白激酶基因的表达受干应激和盐诱导。 通过过表达或抑制蛋白激酶基因来控制作物的耐环境性。 通过用含有蛋白激酶基因的表达载体转化植物来产生脱水增加的转基因植物。
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86.发明公开벼 유래 ＯｓＣＫ1 유전자, 이 유전자를 포함하는발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이형질전환체의 제조방법 有权来自ORYZA SATIVA的OSCK1基因，包含基因的表达载体，转化体转化的转化体和转化体的生产方法
公开(公告)号：KR1020060104772A
公开(公告)日：2006-10-09
申请号：KR1020050027204
申请日：2005-03-31
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 벼 유래 OsCK1 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것으로서, 더욱 상세하게는, 벼 내에서 과다발현시키면 벼의 벼흰잎마름병 및 벼도열병에 대한 저항성이 높아지는, 벼 유래 OsCK1을 코딩하는 서열번호 1로 기재되는 염기서열로 이루어지는 유전자, 이 유전자를 포함하는 발현벡터 pSBM-LS28, 이 발현벡터로 형질전환된 아그로박테리움 형질전환체(수탁번호:KACC 95034P) 및 벼 형질전환체 및 이 형질전환체의 제조방법에 관한 것이다.
벼, 콜린 카이나제, 형질전환체, 벼 도열병 저항성, 벼흰잎마름병 저항성-
公开(公告)号：KR100435143B1
公开(公告)日：2004-06-14
申请号：KR1020010012040
申请日：2001-03-08
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/11
Abstract: PURPOSE: Provided are plant tissue specific promoters, thereby controlling when, where and how much to express a foreign gene. CONSTITUTION: A pollen specific promoter region Pban102 contains 1,177 bp upstream of an initiation codon ATG in a genomic clone of BAN102 gene having the nucleotide sequence of SEQ ID NO: 1. A pollen superior promoter region Pban103 contains 1 to 1831 bp in a genomic clone of BAN103 gene having the nucleotide sequence of SEQ ID NO: 2. A pollen specific promoter region P84 contains 1 to 565 bp in a genomic clone of BAN84 gene having SEQ ID NO: 3. Plasmids, pGBAN84, p84Xb, p84Bm and p84SM, contain the genomic clone of Chinese cabbage containing the BAN84 gene. A carpet tissue specific promoter region PBcA9 contains 616 to 1,436 bp in a genomic clone of BcA9 gene having the nucleotide sequence of SEQ ID NO: 4. A vascular bundle specific promoter region Pbif38 contains 1 to 966 bp in a gemonic clone of BIF38 gene having the nucleotide sequence of SEQ ID NO: 5.
Abstract translation: 目的：提供植物组织特异性启动子，从而控制表达外源基因的时间，地点和数量。 构成：花粉特异性启动子区Pban102含有具有SEQ ID NO：1的核苷酸序列的BAN102基因的基因组克隆中起始密码子ATG的上游1,177bp。花粉上位启动子区Pban103在基因组克隆中含有1至1831bp 具有SEQ ID NO：2的核苷酸序列的BAN103基因。在具有SEQ ID NO：3的BAN84基因的基因组克隆中，花粉特异性启动子区P84含有1至565bp。质粒pGBAN84，p84Xb，p84Bm和p84SM含有 含有BAN84基因的大白菜基因组克隆。 在具有SEQ ID NO：4的核苷酸序列的BcA9基因的基因组克隆中，地毯组织特异性启动子区域PBcA9含有616至1,436bp。维管束特异性启动子区域Pbif38在BIF38基因的基因克隆中含有1至966bp， SEQ ID NO：5的核苷酸序列。
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