公开(公告)号：WO9904228A2

公开(公告)日：1999-01-28

申请号：PCT/US9814575

申请日：1998-07-15

Applicant: LJL BIOSYSTEMS INC ,  BURTON WILLIAM G ,  CRAWFORD KIMBERLY L ,  DESHPANDE SUDHIR S ,  EDWARDS GLENN R ,  EL HAGE AMER ,  FRENCH TODD E ,  LEMBERG VLADIMIR ,  LEYTES JOSEPH S ,  MARQUISS SAMUEL A ,  MEADOWS JOHN W ,  MINEYEV IRINA ,  MODLIN DOUGLAS N ,  OWICKI JOHN C ,  PETERSON JON F ,  RICHARDSON DERRICK A ,  STELLMACHER RICK V ,  TAYLOR MICHAEL T

Inventor： BURTON WILLIAM G ,  CRAWFORD KIMBERLY L ,  DESHPANDE SUDHIR S ,  EDWARDS GLENN R ,  EL-HAGE AMER ,  FRENCH TODD E ,  LEMBERG VLADIMIR ,  LEYTES JOSEPH S ,  MARQUISS SAMUEL A ,  MEADOWS JOHN W ,  MINEYEV IRINA ,  MODLIN DOUGLAS N ,  OWICKI JOHN C ,  PETERSON JON F ,  RICHARDSON DERRICK A ,  STELLMACHER RICK V ,  TAYLOR MICHAEL T

IPC: G01N21/64 ,  B01L9/00 ,  C40B60/14 ,  F21V21/18 ,  G01N21/03 ,  G01N21/17 ,  G01N21/21 ,  G01N21/27 ,  G01N21/76 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01J

CPC classification number: G01N21/76 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01L9/523 ,  B01L2300/0829 ,  B82Y15/00 ,  C40B60/14 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/115831 ,  Y10T436/25

Abstract: A high-throughput light detection instrument and method are described. In some embodiments, switch mechanisms and optical relay structures permit different light sources and/or detectors to be selected for different applications. In other embodiments, switch mechanisms and optical paths permit top/bottom illumination and/or top/bottom detection, or combinations thereof.

Abstract translation: 描述了高通量光检测仪器和方法。 在一些实施例中，开关机构和光学继电器结构允许为不同的应用选择不同的光源和/或检测器。 在其他实施例中，开关机构和光路允许顶部/底部照明和/或顶部/底部检测或其组合。

公开(公告)号：WO9937203A9

公开(公告)日：1999-11-18

申请号：PCT/US9901656

申请日：1999-01-25

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

IPC: B01L9/00 ,  C40B60/14 ,  G01N21/64 ,  G01N33/15 ,  G01N35/02 ,  A61B

CPC classification number: G01N21/6452 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01L9/523 ,  B82Y15/00 ,  C40B60/14 ,  G01N35/028 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Apparatus, methods, and compositions of matter for improving signal resolution in optical spectroscopy assays. The apparatus may include components for detecting light emitted by an analyte in a composition. These components may include (1) a stage for supporting the composition, (2) a light source and a first optical relay structure that directs light from the light source toward the composition, so that the analyte may be induced to emit light, (3) a detector and a second optical relay structure that directs light from the composition toward the detector, so that the light may be detected and converted to a signal, and (4) a processor for analyzing the signal. The processor may be used to discriminate between a first portion of the signal that is attributable to the light emitted by the analyte and a second portion of the signal that is attributable to a non-analyte emitter. The non-analyte emitter may include background, and/or the non-analyte emitter may include a reference compound for correcting scattering and absorption, among others.

Abstract translation: 用于改善光学光谱测定中信号分辨率的物质的装置，方法和组成。 该装置可以包括用于检测由组合物中的分析物发射的光的组件。 这些部件可以包括（1）用于支撑组合物的载物台，（2）将来自光源的光引向组合物的光源和第一光学中继结构，使得分析物可被感光发光（3 ）检测器和将组合物的光引向检测器的第二光学继电器结构，使得可以检测光并将其转换为信号，以及（4）用于分析信号的处理器。 处理器可以用于区分可归因于由分析物发射的光的信号的第一部分和可归因于非分析物发射器的信号的第二部分。 非分析物发射体可以包括背景，和/或非分析物发射体可以包括用于校正散射和吸收的参考化合物等。

公开(公告)号：WO0042209A9

公开(公告)日：2001-11-08

申请号：PCT/US0000895

申请日：2000-01-14

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  FRENCH TODD E ,  PETERSEN JON F ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  FRENCH TODD E ,  PETERSEN JON F ,  OWICKI JOHN C

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C40B60/14 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  C12P19/34

CPC classification number: G01N35/028 ,  B01J2219/00317 ,  B01J2219/00529 ,  B01L3/50853 ,  C12Q1/6816 ,  C12Q1/6827 ,  C40B60/14 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/0405 ,  G01N2035/0425 ,  G01N2035/0462 ,  Y10T436/143333 ,  C12Q2565/107 ,  C12Q2563/103 ,  C12Q2561/119

Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) contacting a sample polynucleotide with a reference polynucleotide at an assay site, where at least one of the polynucleotides is capable of emitting luminescence, (2) illuminating the assay site with light capable of stimulating such luminescence, (3) detecting light transmitted from the assay site, and (4) deriving information relating to the extent of hybridization between the sample and reference polynucleotides based on the detected light. The methods may further include (1) illuminating with and/or detecting polarized light, (2) deriving information relating to the sequence of the sample polynucleotide from the extent of hybridization, and (3) converting the light to a signal and distinguishing between a portion of the signal attributable to luminescence and a portion attributable to background.

Abstract translation: 用于在基于发光的测定中检测多核苷酸杂交的方法和装置。 方法可以包括（1）在测定位点使样品多核苷酸与参考多核苷酸接触，其中至少一个多核苷酸能够发射发光，（2）用能够刺激这种发光的光照射测定位点，（3 ）检测从测定位点传输的光，以及（4）基于检测到的光导出与样品和参考多核苷酸之间的杂交程度相关的信息。 所述方法还可以包括（1）用偏振光进行照射和/或检测偏振光，（2）从杂交程度导出与样品多核苷酸序列相关的信息，和（3）将光转换成信号并区分 可归因于发光的信号的一部分和归因于背景的部分。

公开(公告)号：WO0006990A9

公开(公告)日：2000-07-27

申请号：PCT/US9916287

申请日：1999-07-26

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

IPC: G01N21/25 ,  G01N21/64

CPC classification number: G01N21/6452 ,  B82Y15/00 ,  G01N21/51 ,  G01N21/6408 ,  G01N21/6445 ,  G01N21/645 ,  G01N2021/6471

Abstract: Apparatus and method for measuring light transmitted from a sample. The apparatus may include a stage (123), a light source (103), and a detector (145). The stage (123) may be configured to hold a microplate (124) having a plurality of sample wells (126). The apparatus may be configured to take frequency-domain time-resolved measurements of one or more of luminescence lifetimes and reorientational correlation times of a luminescent analyte in the sample.

Abstract translation: 用于测量从样本发射的光的设备和方法。 该装置可以包括平台（123），光源（103）和检测器（145）。 台（123）可以构造成保持具有多个样品阱（126）的微板（124）。 该装置可以被配置成对样品中的发光分析物的发光寿命和重新定向相关时间中的一个或多个进行频域时间分辨测量。

公开(公告)号：WO0006989A3

公开(公告)日：2000-03-23

申请号：PCT/US9916286

申请日：1999-07-26

Applicant: LJL BIOSYSTEMS INC ,  OWICKI JOHN C ,  MODLIN DOUGLAS N ,  FRENCH TODD E

Inventor： OWICKI JOHN C ,  MODLIN DOUGLAS N ,  FRENCH TODD E

IPC: G01N21/64 ,  G01N21/76

CPC classification number: G01N21/6408 ,  G01N21/76

Abstract: Apparatus and methods for identifying and correcting for quenching in luminescence assays using luminescence lifetimes and/or luminescence intensities. One aspect of the invention involves identifying quenching using combinations of luminescence lifetimes and/or intensities. Another aspect of the invention involves correcting for quenching by eliminating false positives or false negatives due to quenching in luminescence assays.

Abstract translation: 用于在发光测定中使用发光寿命和/或发光强度来鉴定和校正猝灭的装置和方法。 本发明的一个方面涉及使用发光寿命和/或强度的组合来识别猝灭。 本发明的另一方面涉及通过消除由于发光测定中的猝灭而导致的假阳性或假阴性来校正猝灭。

公开(公告)号：WO0006990A2

公开(公告)日：2000-02-10

申请号：PCT/US9916287

申请日：1999-07-26

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

IPC: G01N21/25 ,  G01N21/64 ,  G01N

CPC classification number: G01N21/6452 ,  B82Y15/00 ,  G01N21/51 ,  G01N21/6408 ,  G01N21/6445 ,  G01N21/645 ,  G01N2021/6471

Abstract: Apparatus and method for measuring light transmitted from a sample. The apparatus may include a stage (123), a light source (103), and a detector (145). The stage (123) may be configured to hold a microplate (124) having a plurality of sample wells (126). The apparatus may be configured to take frequency-domain time-resolved measurements of one or more of luminescence lifetimes and reorientational correlation times of a luminescent analyte in the sample.

Abstract translation: 用于测量从样品透射的光的装置和方法。 该装置可以包括台（123），光源（103）和检测器（145）。 级（123）可以被配置成保持具有多个样品阱（126）的微孔板（124）。 该装置可以被配置为进行样品中发光分析物的发光寿命和重新定向相关时间中的一个或多个的频域时间分辨测量。