公开(公告)号：WO2007131194A2

公开(公告)日：2007-11-15

申请号：PCT/US2007/068285

申请日：2007-05-04

Applicant: NANONEXUS, INC. ,  BOTTOMS, W.R. ,  CHONG, Fu, Chiung ,  MILTER, Roman ,  DINAN, Thomas, Edward ,  CHIEH, Erh-Kong ,  DOAN, David, Thanh ,  GIAUQUE, Pierre, H. ,  MODLIN, Douglas, N.

Inventor： BOTTOMS, W.R. ,  CHONG, Fu, Chiung ,  MILTER, Roman ,  DINAN, Thomas, Edward ,  CHIEH, Erh-Kong ,  DOAN, David, Thanh ,  GIAUQUE, Pierre, H. ,  MODLIN, Douglas, N.

IPC: G01R31/28

CPC classification number: G01R31/2889 ,  G01R1/06744 ,  H01L2924/14

Abstract: Enhanced optical probe structures and associated methods comprise improvements to spring structures, wherein optical signals can be transmitted through the resultant substrate by fabricating openings of sufficient size through the substrate through which the optical signals can be transmitted. The holes may be unfilled or filled with optically conducting materials including but not limited to polymers, glasses, air, vacuum, etc. Lenses, diffraction gratings and other optical elements, e.g. refractive or diffractive, can be integrated to improve the coupling efficiency or provide frequency discrimination as desired. Enhanced spring structures and associated methods are also used in conjunction with the enhanced optical probe architectures, such as for processing, testing, and/or packaging of semiconductor ICs and image sensors.

Abstract translation: 增强的光学探针结构和相关方法包括对弹簧结构的改进，其中光学信号可以通过制造足够尺寸的开口通过所得到的衬底传输通过衬底，通过该衬底可以传输光学信号。 孔可以是未填充的或用光学材料填充，包括但不限于聚合物，玻璃，空气，真空等。透镜，衍射光栅和其它光学元件，例如。 折射或衍射可以被整合以改善耦合效率或者根据需要提供频率鉴别。 增强的弹簧结构和相关联的方法也与增强的光学探针结构一起使用，例如用于半导体IC和图像传感器的处理，测试和/或封装。

公开(公告)号：WO9954711A8

公开(公告)日：2000-02-10

申请号：PCT/US9908410

申请日：1999-04-16

Applicant: LJL BIOSYSTEMS INC ,  BURTON WILLIAM G ,  MODLIN DOUGLAS N ,  RICHARDSON DERRICK A ,  PETERSON JON F ,  LEYTES JOSEPH S ,  OWICKI JOHN C ,  EL HAGE AMER

Inventor： BURTON WILLIAM G ,  MODLIN DOUGLAS N ,  RICHARDSON DERRICK A ,  PETERSON JON F ,  LEYTES JOSEPH S ,  OWICKI JOHN C ,  EL-HAGE AMER

IPC: B01L9/00 ,  C40B60/14 ,  G01N21/03 ,  G01N35/02

CPC classification number: B01L9/523 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01L2300/0829 ,  B82Y15/00 ,  C40B60/14 ,  G01N21/0303 ,  G01N35/028 ,  Y10T436/11

Abstract: Sample-holding devices (124) and systems (90) for efficiently utilizing relatively small-volume fluid samples.

Abstract translation: 用于有效利用相对小体积的流体样品的样品保持装置（124）和系统（90）。

公开(公告)号：WO0004364A2

公开(公告)日：2000-01-27

申请号：PCT/US9916057

申请日：1999-07-15

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N

Inventor： MODLIN DOUGLAS N

IPC: G01N21/55 ,  G01N21/64 ,  G01N35/02 ,  G01N

CPC classification number: G01N21/6452 ,  B82Y15/00 ,  G01N21/6408 ,  G01N21/648 ,  G01N35/028

Abstract: Devices and methods are disclosed comprising providing a sample holder (41) having a plurality of sample wells (44). Inner (46) and outer (48) surfaces of at least one well are configured for total internal reflection of incident light (i).

Abstract translation: 公开了包括提供具有多个样品阱（44）的样品架（41）的装置和方法。 至少一个阱的内部（46）和外部（48）表面被配置成用于入射光（i）的全内反射。

公开(公告)号：WO9937203A9

公开(公告)日：1999-11-18

申请号：PCT/US9901656

申请日：1999-01-25

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

IPC: B01L9/00 ,  C40B60/14 ,  G01N21/64 ,  G01N33/15 ,  G01N35/02 ,  A61B

CPC classification number: G01N21/6452 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01L9/523 ,  B82Y15/00 ,  C40B60/14 ,  G01N35/028 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Apparatus, methods, and compositions of matter for improving signal resolution in optical spectroscopy assays. The apparatus may include components for detecting light emitted by an analyte in a composition. These components may include (1) a stage for supporting the composition, (2) a light source and a first optical relay structure that directs light from the light source toward the composition, so that the analyte may be induced to emit light, (3) a detector and a second optical relay structure that directs light from the composition toward the detector, so that the light may be detected and converted to a signal, and (4) a processor for analyzing the signal. The processor may be used to discriminate between a first portion of the signal that is attributable to the light emitted by the analyte and a second portion of the signal that is attributable to a non-analyte emitter. The non-analyte emitter may include background, and/or the non-analyte emitter may include a reference compound for correcting scattering and absorption, among others.

Abstract translation: 用于改善光学光谱测定中信号分辨率的物质的装置，方法和组成。 该装置可以包括用于检测由组合物中的分析物发射的光的组件。 这些部件可以包括（1）用于支撑组合物的载物台，（2）将来自光源的光引向组合物的光源和第一光学中继结构，使得分析物可被感光发光（3 ）检测器和将组合物的光引向检测器的第二光学继电器结构，使得可以检测光并将其转换为信号，以及（4）用于分析信号的处理器。 处理器可以用于区分可归因于由分析物发射的光的信号的第一部分和可归因于非分析物发射器的信号的第二部分。 非分析物发射体可以包括背景，和/或非分析物发射体可以包括用于校正散射和吸收的参考化合物等。

公开(公告)号：WO9904228A9

公开(公告)日：1999-07-08

申请号：PCT/US9814575

申请日：1998-07-15

Applicant: LJL BIOSYSTEMS INC ,  BARKER CRAIG S ,  BECHTEL LORNE B ,  BURTON WILLIAM G ,  EDWARDS GLENN R ,  EL HAGE AMER ,  GRANIERI PHILIP A JR ,  LEMBI ROBERT M SR ,  LEYTES JOSEPH S ,  LEYTES LEV J ,  MARQUISS SAMUEL A ,  MEADOWS JOHN W ,  MODLIN DOUGLAS N ,  OWICKI JOHN C ,  PAIK YONG ,  RICHARDSON DERRICK A ,  STELLMACHER RICK V ,  TAYLOR MICHAEL T ,  WONG CALVIN D

Inventor： BARKER CRAIG S ,  BECHTEL LORNE B ,  BURTON WILLIAM G ,  EDWARDS GLENN R ,  EL-HAGE AMER ,  GRANIERI PHILIP A JR ,  LEMBI ROBERT M SR ,  LEYTES JOSEPH S ,  LEYTES LEV J ,  MARQUISS SAMUEL A ,  MEADOWS JOHN W ,  MODLIN DOUGLAS N ,  OWICKI JOHN C ,  PAIK YONG ,  RICHARDSON DERRICK A ,  STELLMACHER RICK V ,  TAYLOR MICHAEL T ,  WONG CALVIN D

IPC: G01N21/64 ,  B01L9/00 ,  C40B60/14 ,  F21V21/18 ,  G01N21/03 ,  G01N21/17 ,  G01N21/21 ,  G01N21/27 ,  G01N21/76 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N21/66

CPC classification number: G01N21/76 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01L9/523 ,  B01L2300/0829 ,  B82Y15/00 ,  C40B60/14 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/115831 ,  Y10T436/25

Abstract: A high-throughput light detection instrument and method are described. In some embodiments, switch mechanisms (108, 136) and optical relay structures permit different light sources (103a-d) and/or detectors (145a-d) to be selected for different applications. In other embodiments, switch mechanisms (108, 136) and optical paths permit top/bottom illumination and/or top/bottom detection, or combinations thereof.

Abstract translation: 描述了高通量光检测仪器和方法。 在一些实施例中，开关机构（108,136）和光学中继结构允许为不同的应用选择不同的光源（103a-d）和/或检测器（145a-d）。 在其他实施例中，开关机构（108,136）和光路允许顶部/底部照明和/或顶部/底部检测或其组合。

公开(公告)号：WO0042209A9

公开(公告)日：2001-11-08

申请号：PCT/US0000895

申请日：2000-01-14

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  FRENCH TODD E ,  PETERSEN JON F ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  FRENCH TODD E ,  PETERSEN JON F ,  OWICKI JOHN C

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C40B60/14 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  C12P19/34

CPC classification number: G01N35/028 ,  B01J2219/00317 ,  B01J2219/00529 ,  B01L3/50853 ,  C12Q1/6816 ,  C12Q1/6827 ,  C40B60/14 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/0405 ,  G01N2035/0425 ,  G01N2035/0462 ,  Y10T436/143333 ,  C12Q2565/107 ,  C12Q2563/103 ,  C12Q2561/119

Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) contacting a sample polynucleotide with a reference polynucleotide at an assay site, where at least one of the polynucleotides is capable of emitting luminescence, (2) illuminating the assay site with light capable of stimulating such luminescence, (3) detecting light transmitted from the assay site, and (4) deriving information relating to the extent of hybridization between the sample and reference polynucleotides based on the detected light. The methods may further include (1) illuminating with and/or detecting polarized light, (2) deriving information relating to the sequence of the sample polynucleotide from the extent of hybridization, and (3) converting the light to a signal and distinguishing between a portion of the signal attributable to luminescence and a portion attributable to background.

Abstract translation: 用于在基于发光的测定中检测多核苷酸杂交的方法和装置。 方法可以包括（1）在测定位点使样品多核苷酸与参考多核苷酸接触，其中至少一个多核苷酸能够发射发光，（2）用能够刺激这种发光的光照射测定位点，（3 ）检测从测定位点传输的光，以及（4）基于检测到的光导出与样品和参考多核苷酸之间的杂交程度相关的信息。 所述方法还可以包括（1）用偏振光进行照射和/或检测偏振光，（2）从杂交程度导出与样品多核苷酸序列相关的信息，和（3）将光转换成信号并区分 可归因于发光的信号的一部分和归因于背景的部分。

公开(公告)号：WO0005336A9

公开(公告)日：2000-11-02

申请号：PCT/US9916453

申请日：1999-07-21

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  EL HAGE AMER ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  EL-HAGE AMER ,  OWICKI JOHN C

IPC: B01L3/00 ,  C12M20060101 ,  G01N1/10 ,  G01N21/00

CPC classification number: B01L3/5085 ,  B01L3/508 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0822 ,  B01L2300/0829

Abstract: Devices (400) and methods for containing and analyzing small volumes (416) that are sandwiched between solid surfaces. The devices (400) may include an automated drive mechanism (424) that controls the relative positions of the surfaces and an environmental-control mechanism (411) that controls the humidity, temperature, and/or other environmental conditions around the small sample volume. In some embodiments, at least one of the surfaces has a light-transmissive window for allowing optical analysis of a sample contained between the surfaces.

公开(公告)号：WO0006990A9

公开(公告)日：2000-07-27

申请号：PCT/US9916287

申请日：1999-07-26

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  FRENCH TODD E ,  OWICKI JOHN C

IPC: G01N21/25 ,  G01N21/64

CPC classification number: G01N21/6452 ,  B82Y15/00 ,  G01N21/51 ,  G01N21/6408 ,  G01N21/6445 ,  G01N21/645 ,  G01N2021/6471

Abstract: Apparatus and method for measuring light transmitted from a sample. The apparatus may include a stage (123), a light source (103), and a detector (145). The stage (123) may be configured to hold a microplate (124) having a plurality of sample wells (126). The apparatus may be configured to take frequency-domain time-resolved measurements of one or more of luminescence lifetimes and reorientational correlation times of a luminescent analyte in the sample.

Abstract translation: 用于测量从样本发射的光的设备和方法。 该装置可以包括平台（123），光源（103）和检测器（145）。 台（123）可以构造成保持具有多个样品阱（126）的微板（124）。 该装置可以被配置成对样品中的发光分析物的发光寿命和重新定向相关时间中的一个或多个进行频域时间分辨测量。

公开(公告)号：WO9923466A3

公开(公告)日：1999-08-19

申请号：PCT/US9823095

申请日：1998-10-30

Applicant: LJL BIOSYSTEMS INC ,  MODLIN DOUGLAS N ,  OWICKI JOHN C

Inventor： MODLIN DOUGLAS N ,  OWICKI JOHN C

IPC: G01J3/447 ,  B01L9/00 ,  C40B60/14 ,  G01N21/21 ,  G01N21/64 ,  G01N33/542 ,  G01N33/58 ,  G01N35/02

CPC classification number: G01N33/588 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01L9/523 ,  B82Y15/00 ,  C40B60/14 ,  G01N21/6408 ,  G01N21/6445 ,  G01N21/6452 ,  G01N33/542 ,  G01N33/582 ,  G01N35/028 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2458/40

Abstract: An apparatus (90) for measuring polarized light emitted from a composition that includes a stage configured to hold a microplate (124) having an array of sample wells (126), a continuous high color temperature light source (100), and a detector (144). An excitation optical relay structure directs light from the light source (100) through an excitation polarizer (114) toward a composition in one of the sample wells (126). An emission optical relay structure directs light emitted from the composition through an emission polarizer (132) toward the detector (144).

Abstract translation: 一种用于测量从组合物发射的偏振光的装置（90），该装置包括一个被配置为保持具有一个样品阱阵列（126）的微板（124），一个连续的高色温光源（100）和一个检测器 144）。 激发光学继电器结构将来自光源（100）的光通过激发偏振器（114）引导到样品阱（126）之一中的组合物。 发射光学继电器结构将从组合物发射的光通过发射偏振器（132）引向检测器（144）。

公开(公告)号：WO2007131194A4

公开(公告)日：2010-10-21

申请号：PCT/US2007068285

申请日：2007-05-04

Applicant: NANONEXUS INC ,  BOTTOMS W R ,  CHONG FU CHIUNG ,  MILTER ROMAN ,  DINAN THOMAS EDWARD ,  CHIEH ERH-KONG ,  DOAN DAVID THANH ,  GIAUQUE PIERRE H ,  MODLIN DOUGLAS N

Inventor： BOTTOMS W R ,  CHONG FU CHIUNG ,  MILTER ROMAN ,  DINAN THOMAS EDWARD ,  CHIEH ERH-KONG ,  DOAN DAVID THANH ,  GIAUQUE PIERRE H ,  MODLIN DOUGLAS N

IPC: H01L31/0224

CPC classification number: G01R31/2889 ,  G01R1/06744 ,  H01L2924/14

Abstract: Enhanced optical probe structures and associated methods comprise improvements to spring structures, wherein optical signals can be transmitted through the resultant substrate by fabricating openings of sufficient size through the substrate through which the optical signals can be transmitted. The holes may be unfilled or filled with optically conducting materials including but not limited to polymers, glasses, air, vacuum, etc. Lenses, diffraction gratings and other optical elements, e.g. refractive or diffractive, can be integrated to improve the coupling efficiency or provide frequency discrimination as desired. Enhanced spring structures and associated methods are also used in conjunction with the enhanced optical probe architectures, such as for processing, testing, and/or packaging of semiconductor ICs and image sensors.

Abstract translation: 增强的光学探针结构和相关方法包括对弹簧结构的改进，其中光学信号可以通过制造足够尺寸的开口通过所得到的衬底传输通过衬底，通过该衬底可以传输光学信号。 孔可以是未填充的或用光学材料填充，包括但不限于聚合物，玻璃，空气，真空等。透镜，衍射光栅和其它光学元件，例如。 折射或衍射可以被整合以改善耦合效率或者根据需要提供频率鉴别。 增强的弹簧结构和相关联的方法也与增强的光学探针结构一起使用，例如用于半导体IC和图像传感器的处理，测试和/或封装。