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1.发明公开POLYMERIZATION OF NUCLEIC ACIDS USING ACTIVATION BY POLYPHOSPHOROLYSIS (APP) REACTIONS 审中-公开多磷酸化反应活化聚合核酸（APP）
公开(公告)号：EP2652152A4
公开(公告)日：2014-06-25
申请号：EP11848096
申请日：2011-12-13
Applicant: LIFE TECHNOLOGIES CORP , XI LEI A K A LARRY , KENNEY PAUL , MA ZHAOCHUN , PROSEN DENNIS , HENDRICKS STEPHEN , NAGEL ROLAND
Inventor： XI LEI A K A LARRY , KENNEY PAUL , MA ZHAOCHUN , PROSEN DENNIS , HENDRICKS STEPHEN , NAGEL ROLAND
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
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2.发明公开POLYMERIZATION OF NUCLEIC ACIDS USING ACTIVATION BY POLYPHOSPHOROLYSIS (APP) REACTIONS 审中-公开多磷酸化（APP）反应活化聚合核酸
公开(公告)号：EP2652152A2
公开(公告)日：2013-10-23
申请号：EP11848096.1
申请日：2011-12-13
Applicant: Life Technologies Corporation , Xi, Lei (a.k.a. Larry) , Kenney, Paul , Ma, Zhaochun , Prosen, Dennis , Hendricks, Stephen , Nagel, Roland
Inventor： XI, Lei (a.k.a. Larry) , KENNEY, Paul , MA, Zhaochun , PROSEN, Dennis , HENDRICKS, Stephen , NAGEL, Roland
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or -monothiopyrophosphate), and related compounds.
Abstract translation: 本公开涉及使用多磷酸化试剂三磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代二磷酸盐（或单硫代焦磷酸盐）和相关化合物中的至少一种通过多磷酸分解（APP）反应进行活化的方法。
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Patent Agency Ranking
公开(公告)号：WO2009029648A2
公开(公告)日：2009-03-05
申请号：PCT/US2008/074432
申请日：2008-08-27
Applicant: APPLERA CORPORATION , XI, Larry, L. , PROSEN, Dennis, E. , NOVIKOV, Alexander, A.
Inventor： XI, Larry, L. , PROSEN, Dennis, E. , NOVIKOV, Alexander, A.
IPC: B32B27/12
CPC classification number: C12N9/1252 , C12N9/88 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y402/99
Abstract: A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula I wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3'-5' exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase.
Abstract translation: 通过在基本水溶液条件下通过热稳定的Pol B DNA聚合酶和式I的改性剂通过反应产生的经修饰的热稳定的Pol B DNA聚合酶，其中所述反应导致热可逆的Pol B DNA聚合酶活性的热可逆失活， 3'-5'核酸外切酶活性，该聚合酶适用于热启动PCR。 还公开了修饰方法，多核酸扩增方法和PCR反应混合物和试剂盒，其包含修饰的热稳定性Pol B DNA聚合酶。
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公开(公告)号：WO2009029648A3
公开(公告)日：2009-04-30
申请号：PCT/US2008074432
申请日：2008-08-27
Applicant: APPLERA CORP , XI LARRY L , PROSEN DENNIS E , NOVIKOV ALEXANDER A
Inventor： XI LARRY L , PROSEN DENNIS E , NOVIKOV ALEXANDER A
IPC: C12P19/34
CPC classification number: C12N9/1252 , C12N9/88 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y402/99
Abstract: A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula (I) wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3'-5' exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase.
Abstract translation: 通过在基本上水溶液条件下通过热稳定的Pol B DNA聚合酶和式（I）的改性剂反应产生的经修饰的热稳定的Pol B DNA聚合酶，其中所述反应导致热可逆的Pol B DNA聚合酶的热可逆失活 活性和3'-5'核酸外切酶活性，该聚合酶适用于热启动PCR。 还公开了修饰方法，多核酸扩增方法和PCR反应混合物和试剂盒，其包含修饰的热稳定性Pol B DNA聚合酶。
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5.发明申请POLYMERIZATION OF NUCLEIC ACIDS USING ACTIVATION BY POLYPHOSPHOROLYSIS (APP) REACTIONS 审中-公开使用多磷酸（APP）反应活化的核酸的聚合
公开(公告)号：WO2012082753A2
公开(公告)日：2012-06-21
申请号：PCT/US2011/064668
申请日：2011-12-13
Applicant: LIFE TECHNOLOGIES CORPORATION , XI, Lei (a.k.a. Larry) , KENNEY, Paul , MA, Zhaochun , PROSEN, Dennis , HENDRICKS, Stephen , NAGEL, Roland
Inventor： XI, Lei (a.k.a. Larry) , KENNEY, Paul , MA, Zhaochun , PROSEN, Dennis , HENDRICKS, Stephen , NAGEL, Roland
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or -monothiopyrophosphate), and related compounds.
Abstract translation: 本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代二磷酸盐（或 - 硫代焦磷酸盐）和相关化合物中的至少一种多磷酸解（APP）反应进行活化的方法。
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公开(公告)号：WO2020190454A1
公开(公告)日：2020-09-24
申请号：PCT/US2020/019506
申请日：2020-02-24
Applicant: CELLULAR RESEARCH, INC. , PROSEN, Dennis
Inventor： PROSEN, Dennis , SPUHLER, Philipp , LI, Sixing
IPC: B01L3/00 , B01L7/00 , C12Q1/6834
Abstract: Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.
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7.发明申请POLYMERIZATION OF NUCLEIC ACIDS USING ACTIVATION BY POLYPHOSPHOROLYSIS (APP) REACTIONS 审中-公开使用多磷酸（APP）反应活化的核酸的聚合
公开(公告)号：WO2012082753A3
公开(公告)日：2013-01-10
申请号：PCT/US2011064668
申请日：2011-12-13
Applicant: LIFE TECHNOLOGIES CORP , XI LEI A K A LARRY , KENNEY PAUL , MA ZHAOCHUN , PROSEN DENNIS , HENDRICKS STEPHEN , NAGEL ROLAND
Inventor： XI LEI A K A LARRY , KENNEY PAUL , MA ZHAOCHUN , PROSEN DENNIS , HENDRICKS STEPHEN , NAGEL ROLAND
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or -monothiopyrophosphate), and related compounds.
Abstract translation: 本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代二磷酸盐（或 - 硫代焦磷酸盐）和相关化合物中的至少一种多磷酸解（APP）反应进行活化的方法。
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