公开(公告)号：CA2789425A1

公开(公告)日：2011-08-18

申请号：CA2789425

申请日：2011-02-11

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

IPC: C12Q1/68 ,  C12N15/10 ,  C12P19/34 ,  C40B30/04

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：WO2011100604A3

公开(公告)日：2014-03-27

申请号：PCT/US2011024615

申请日：2011-02-11

Applicant: RAINDANCE TECHNOLOGIES INC ,  LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

Inventor： LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Abstract translation: 本发明总体上涉及基于微滴的数字PCR和使用其分析靶核酸的方法。 在某些实施方案中，本发明的方法涉及形成平均含有单个靶核酸的样品液滴，扩增液滴中的靶，排除含有来自靶的扩增子的微滴和来自靶变体的扩增子，并分析靶扩增子 。

公开(公告)号：WO2012109600A3

公开(公告)日：2013-11-28

申请号：PCT/US2012024741

申请日：2012-02-10

Applicant: RAINDANCE TECHNOLOGIES INC ,  YURKOVETSKY YEVGENY ,  LINK DARREN ,  LARSON JONATHAN WILLIAM

Inventor： YURKOVETSKY YEVGENY ,  LINK DARREN ,  LARSON JONATHAN WILLIAM

IPC: C12Q1/68

CPC classification number: B01F13/0076 ,  B01F3/0803 ,  B01F3/0865 ,  B01F5/0085 ,  B01F5/0471 ,  B01F5/0473 ,  B01F13/0062 ,  B01F13/0071 ,  B01F2215/0037 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487

Abstract: Methods involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet. Methods for merging two liquid phases in which only one phase is in the form of a droplet at least at the point of merging A second phase is injected into the drops directly from a continuous stream. Methods of the invention provide a simple and reliable approach to sample fluid mixing because only one of the two phases is dispersed as a droplet prior to its merge with the other phase.

Abstract translation: 方法包括形成液滴并使液滴与流体流接触，其中流体流的一部分与液滴一体化以形成混合液滴。 用于合并两个液相的方法，其中至少在合并点只有一相是液滴的形式将第二相直接从连续流注入到液滴中。 本发明的方法提供了一种简单可靠的样品流体混合方法，因为在与其它相合并之前，两相中只有一相作为液滴分散。

公开(公告)号：EP2534267A4

公开(公告)日：2015-05-20

申请号：EP11742907

申请日：2011-02-11

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Abstract translation: 本发明一般涉及基于液滴的数字PCR和用于分析使用其的靶核酸的方法。 在某些实施方案中，本发明的方法包括形成样品液滴，其平均包含单个靶核酸，扩增液滴中的靶标，排除来自靶标的扩增子的液滴和来自靶标变体的扩增子，并分析靶扩增子 。

公开(公告)号：EP2673614A4

公开(公告)日：2016-05-11

申请号：EP12745382

申请日：2012-02-10

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： YURKOVETSKY YEVGENY ,  LINK DARREN ,  LARSON JONATHAN WILLIAM

IPC: C12Q1/68

CPC classification number: B01F13/0076 ,  B01F3/0803 ,  B01F3/0865 ,  B01F5/0085 ,  B01F5/0471 ,  B01F5/0473 ,  B01F13/0062 ,  B01F13/0071 ,  B01F2215/0037 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487

Abstract: The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.

Abstract translation: 本发明一般涉及形成混合液滴的方法。 在某些实施方案中，本发明的方法包括形成液滴并使液滴与流体流接触，其中流体流的一部分与液滴形成混合液滴。