公开(公告)号：CA2789425A1

公开(公告)日：2011-08-18

申请号：CA2789425

申请日：2011-02-11

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

IPC: C12Q1/68 ,  C12N15/10 ,  C12P19/34 ,  C40B30/04

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：WO2011100604A3

公开(公告)日：2014-03-27

申请号：PCT/US2011024615

申请日：2011-02-11

Applicant: RAINDANCE TECHNOLOGIES INC ,  LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

Inventor： LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Abstract translation: 本发明总体上涉及基于微滴的数字PCR和使用其分析靶核酸的方法。 在某些实施方案中，本发明的方法涉及形成平均含有单个靶核酸的样品液滴，扩增液滴中的靶，排除含有来自靶的扩增子的微滴和来自靶变体的扩增子，并分析靶扩增子 。

公开(公告)号：EP2534267A4

公开(公告)日：2015-05-20

申请号：EP11742907

申请日：2011-02-11

Applicant: RAINDANCE TECHNOLOGIES INC

Inventor： LARSON JONATHAN WILLIAM ,  ZHONG QUN ,  LINK DARREN ROY

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Abstract translation: 本发明一般涉及基于液滴的数字PCR和用于分析使用其的靶核酸的方法。 在某些实施方案中，本发明的方法包括形成样品液滴，其平均包含单个靶核酸，扩增液滴中的靶标，排除来自靶标的扩增子的液滴和来自靶标变体的扩增子，并分析靶扩增子 。