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    DNA METHYLATION DETECTION METHODS
    1.
    发明申请
    DNA METHYLATION DETECTION METHODS 审中-公开
    DNA甲基化检测方法

    公开(公告)号：WO2010022098A2

    公开(公告)日：2010-02-25

    申请号：PCT/US2009054220

    申请日：2009-08-18

    Applicant: LIFE TECHNOLOGIES CORP ,  DONG SHOULIAN ,  STEVENS JUNKO

    Inventor： DONG SHOULIAN ,  STEVENS JUNKO

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6827 ,  C12Q2537/164 ,  C12Q2522/101

    Abstract: The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.

    Abstract translation: 本教导提供了避免亚硫酸氢盐处理DNA的DNA甲基化定量方法。 甲基化特异性结合蛋白（MeDNA结合蛋白）和非甲基化特异性结合蛋白（非MeDNA结合蛋白）用于各种实施方案中以调节核酸对引物延伸反应的可及性。 在选择性除去靶核酸后，可以分析延伸产物并定量甲基化。 在一些实施方案中，分析包括实时PCR。

    METHOD FOR OPERATING A COMPUTER AND/OR COMPUTER NETWORK TO DISTRIBUTE BIOTECHNOLOGY PRODUCTS
    2.
    发明申请
    METHOD FOR OPERATING A COMPUTER AND/OR COMPUTER NETWORK TO DISTRIBUTE BIOTECHNOLOGY PRODUCTS 审中-公开
    用于操作计算机和/或计算机网络以分布生物技术产品的方法

    公开(公告)号：WO2003064694A1

    公开(公告)日：2003-08-07

    申请号：PCT/US2002/034599

    申请日：2002-10-30

    Applicant: APPLERA CORPORATION ,  KOEHLER, Ryan, T. ,  LIVAK, Kenneth, J. ,  STEVENS, Junko ,  DE LA VEGA, Francisco, M. ,  RHODES, Michael ,  BELLON, Laurent, R.

    Inventor： KOEHLER, Ryan, T. ,  LIVAK, Kenneth, J. ,  STEVENS, Junko ,  DE LA VEGA, Francisco, M. ,  RHODES, Michael ,  BELLON, Laurent, R.

    IPC: C12Q1/68

    CPC classification number: C12Q1/686

    Abstract: A method for distributing a product to a consumer includes utilizing a computer network to interact with the consumer to obtain information that includes at least one target nucleic acid sequence; providing a forward primer sequence, a reverse primer sequence and a probe sequence having specified characteristics, wherein the forward primer sequence and the reverse primer sequence together define an amplicon sequence, the amplicon lies within the target nucleic acid sequence, and the probe sequence is complementary to a portion of the amplicon sequence; manufacturing at least one assay that includes a forward primer in accordance with the forward primer sequence, a reverse primer in accordance with the reverse primer sequence, and a probe in accordance with the probe sequence; validating one or more of the forward primer, the reverse primer, and the probe; and delivering the manufactured assay to the consumer.

    Abstract translation: 将产品分发给消费者的方法包括利用计算机网络与消费者进行交互以获得包括至少一个靶核酸序列的信息; 提供具有特定特征的正向引物序列，反向引物序列和探针序列，其中正向引物序列和反向引物序列共同限定扩增子序列，扩增子位于靶核酸序列内，探针序列互补 到扩增子序列的一部分; 制备至少一种包括根据正向引物序列的正向引物，根据反向引物序列的反向引物和根据探针序列的探针的测定法; 验证一个或多个正向引物，反向引物和探针; 并将所制造的测定物递送给消费者。

    DNA METHYLATION DETECTION METHODS
    6.
    发明申请
    DNA METHYLATION DETECTION METHODS 审中-公开

    公开(公告)号：WO2010022098A3

    公开(公告)日：2010-02-25

    申请号：PCT/US2009/054220

    申请日：2009-08-18

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  DONG, Shoulian ,  STEVENS, Junko

    Inventor： DONG, Shoulian ,  STEVENS, Junko

    IPC: C12Q1/68 ,  C12N15/11

    Abstract: The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.

    COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS
    7.
    发明申请
    COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS 审中-公开
    用于放大核酸的组合物，方法和试剂盒

    公开(公告)号：WO2007041201A3

    公开(公告)日：2007-11-22

    申请号：PCT/US2006037829

    申请日：2006-09-29

    Applicant: APPLERA CORP ,  DONG SHOULIAN ,  STEVENS JUNKO ,  LEE DANNY H

    Inventor： DONG SHOULIAN ,  STEVENS JUNKO ,  LEE DANNY H

    IPC: C12P19/34

    CPC classification number: C12Q1/6846 ,  C12N15/115 ,  C12N2310/16 ,  C12P19/34 ,  C12Q1/6848 ,  G01N21/6486 ,  C12Q2549/125 ,  C12Q2527/127 ,  C12Q2527/107

    Abstract: The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

    Abstract translation: 本教导涉及用于扩增靶核酸同时减少非特异性荧光和不期望的扩增产物（有时称为二次扩增产物或杂散副产物）的组合物，方法和试剂盒。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了用于扩增靶核酸同时减少不需要的扩增产物的方法，以及用于减少非特异性荧光的方法。 还提供了用于加速某些公开方法的性能的试剂盒。

    COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS
    8.
    发明申请
    COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS 审中-公开
    用于放大核酸的组合物，方法和试剂盒

    公开(公告)号：WO2007041201A2

    公开(公告)日：2007-04-12

    申请号：PCT/US2006/037829

    申请日：2006-09-29

    Applicant: APPLERA CORPORATION ,  DONG, Shoulian ,  STEVENS, Junko ,  LEE, Danny H.

    Inventor： DONG, Shoulian ,  STEVENS, Junko ,  LEE, Danny H.

    IPC: C12Q1/68

    CPC classification number: C12Q1/6846 ,  C12N15/115 ,  C12N2310/16 ,  C12P19/34 ,  C12Q1/6848 ,  G01N21/6486 ,  C12Q2549/125 ,  C12Q2527/127 ,  C12Q2527/107

    Abstract: The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

    Abstract translation: 本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不期望的扩增产物，有时称为二级扩增产物或假副产物。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。 还提供了用于加速某些公开方法的执行的套件。

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