公开(公告)号：WO2011041703A3

公开(公告)日：2011-10-27

申请号：PCT/US2010051165

申请日：2010-10-01

Applicant: LIFE TECHNOLOGIES CORP ,  BREVNOV MAXIM G ,  NURSE JAMES C ,  STRAY JAMES

Inventor： BREVNOV MAXIM G ,  NURSE JAMES C ,  STRAY JAMES

IPC: G01N1/28 ,  G01N33/48

CPC classification number: G01N1/4005 ,  G01N2001/4016

Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.

Abstract translation: 用于样本处理的装置可以包括具有出口的至少一个腔室，所述腔室构造成接收用于处理的样本，过滤器，至少一个腔室中的至少一些样本部分流过所述过滤器，以及设置在 第一状态以将样品容纳在至少一个腔室中。 在足够的条件下，阻挡构件可以改变到第二状态，以允许容纳在腔室中的至少一些样品部分在流动方向上流向出口并且通过过滤器。

公开(公告)号：WO2011041703A2

公开(公告)日：2011-04-07

申请号：PCT/US2010/051165

申请日：2010-10-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BREVNOV, Maxim G. ,  NURSE, James C. ,  STRAY, James

Inventor： BREVNOV, Maxim G. ,  NURSE, James C. ,  STRAY, James

IPC: G01N1/28 ,  G01N33/48

CPC classification number: G01N1/4005 ,  G01N2001/4016

Abstract: A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.

Abstract translation: 用于样品处理的装置可以包括具有出口的至少一个室，所述室被配置为接收用于处理的样品，过滤器，所述至少一个室中的至少一些样品部分通过所述过滤器和设置在所述室中的阻挡构件 第一状态以在至少一个室中容纳样品。 在足够的条件下，阻挡构件可以改变到第二状态，以允许包含在腔室中的至少一些样品部分沿流动方向流向出口并通过过滤器。

公开(公告)号：WO2011034864A1

公开(公告)日：2011-03-24

申请号：PCT/US2010/048833

申请日：2010-09-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  STRAY, James ,  LIU, Yingjie (Jason)

Inventor： STRAY, James ,  LIU, Yingjie (Jason)

IPC: C12N1/06 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12N1/06 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

Abstract translation: 本教导涉及从固体材料中提取核酸。 提供了用于从固体生物样品获得核酸的有用的组合物，方法和试剂盒或具有粘附或嵌入粘合剂基底内的生物材料的粘合剂材料。 提取的核酸可用于下游应用，例如生物材料来源的基因分型，检测，定量和鉴定。