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    Substance-retaining cereal
    1.
    发明专利
    Substance-retaining cereal 未知

    公开(公告)号：AU2010274331A1

    公开(公告)日：2012-02-09

    申请号：AU2010274331

    申请日：2010-07-22

    Applicant: UNIV OSAKA PREFECTURE

    Inventor： KITAMURA SHINICH ,  NAKAYA MAKOTO ,  KUBO AKIKO ,  INUKAI TADAHIKO

    IPC: A23L7/10 ,  A23L7/196

    Abstract: Disclosed are rice grains in which various water-soluble polymeric substances can be retained without applying pressure to the rice grains. Mutant rice grains (wx/ae rice grains) which are deficient in both an amylopectin branching enzyme (BEIIb) and amylose synthase I (GBSSI) are immersed in an aqueous solution of a water-soluble polymeric substance such as indigestible dextrin, arabinogalactan and polyphenol at ambient pressure and room temperature, and subsequently the mutant rice grains are dried at a temperature of 60°C or lower, preferably about 50°C, thereby producing rice grains which are impregnated with the polymeric substance or retain the polymeric substance.

    DETECTION OF BACTERIA BELONGING TO THE GENUS CAMPYLOBACTER TARGETING CELL EXPANSION LETHAL TOXIN
    3.
    发明专利
    DETECTION OF BACTERIA BELONGING TO THE GENUS CAMPYLOBACTER TARGETING CELL EXPANSION LETHAL TOXIN 未知

    公开(公告)号：CA2718214A1

    公开(公告)日：2009-03-05

    申请号：CA2718214

    申请日：2008-02-26

    Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

    Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO

    IPC: C12Q1/68 ,  C12N15/09 ,  C12Q1/04

    Abstract: Multiplex PCR primers that can amplify the cdt genes of C. jejuni, C. coli, and C. fetus in a bacterial species-specific manner were prepared. Multiplex PCR with the primers was assessed using Campylobacter bacteria, other cdt gene-positive bacteria, and representative bacteria responsible for enteric infection. As a result, the present inventors' multiplex PCR using cdtB amplification primers was proven to enable simultaneous detection of different Campylobacter bacteria with high specificity. The methods of the present invention can identify Campylobacter at the bacterial species level in a single manipulation even when domestic animals or humans are infected with different bacterial species of Campylobacter.

    Detection of bacteria belonging to the genus Campylobacter targeting cell expansion lethal toxin
    4.
    发明专利
    Detection of bacteria belonging to the genus Campylobacter targeting cell expansion lethal toxin 未知

    公开(公告)号：AU2008292946A1

    公开(公告)日：2009-03-05

    申请号：AU2008292946

    申请日：2008-02-26

    Applicant: FUSO PHARMACEUTICAL IND ,  UNIV OSAKA PREFECTURE

    Inventor： ASAKURA MASAHIRO ,  YAMASAKI SHINJI

    IPC: C12Q1/68 ,  C12N15/09 ,  C12Q1/04

    Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C. hyointestinalis and polynucleotides encoding it, and novel methods for detection of C. hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter-like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni, C. coli, and C. fetus, but not by multiplex PCR that can specifically detect the cdtA, cdtB, and cdtC genes of the three bacterial species. The bacterial strain was identified as C. hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

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