公开(公告)号：AU2008292375A2

公开(公告)日：2010-04-29

申请号：AU2008292375

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

Inventor： SAMOSORUNSUK WORADA ,  ASAKURA MASAHIRO ,  YAMASAKI SHINJI

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C . hyointestinalis and polynucleotides encoding it, and novel methods for detection of C . hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter -like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni , C. coli, and C . fetus, but not by multiplex PCR that can specifically detect the cdtA , cdtB , and cdtC genes of the three bacterial species. The bacterial strain was identified as C . hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

公开(公告)号：EP2192184A4

公开(公告)日：2011-08-17

申请号：EP08828171

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND INC

Inventor： YAMASAKI SHINJI ,  SAMOSORUNSUK WORADA ,  ASAKURA MASAHIRO

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

CPC classification number: C12Q1/689 ,  C07K16/1203 ,  C07K16/121 ,  C07K2317/76 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/56922 ,  G01N2333/205 ,  Y02A50/451

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C . hyointestinalis and polynucleotides encoding it, and novel methods for detection of C . hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter -like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni , C. coli, and C . fetus, but not by multiplex PCR that can specifically detect the cdtA , cdtB , and cdtC genes of the three bacterial species. The bacterial strain was identified as C . hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

公开(公告)号：CA2697480A1

公开(公告)日：2009-03-05

申请号：CA2697480

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  SAMOSORUNSUK WORADA ,  ASAKURA MASAHIRO

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

Abstract: Disclosed are: a cytolethal distending toxin (CDT) derived from C. hyointestinalis; a polynucleotide encoding the cytolethal distending toxin; and a novel method for detecting C. hyointestinalis by using cdt gene. Attention is focused on a cytolethal distending toxin (CDT) derived from a bacterium belonging to the genus Campylobacter. As a result, a cell line is found whose cdtB gene cannot be amplified by a multiplex PCR method that can detect specifically cdtA gene, cdtB gene and cdtC gene of C. jejuni, C. coli and C. fetus but can be amplified with a primer common to all of these three cell lines,by detecting cdt gene from a bacterium belonging to the genus Campylobacter isolated from a Thai enteritis patient. The analysis is made on 16S rRNA gene, and the cell line is identified as C. hyointestinalis. The full-length nucleotide sequence for cdt gene is determined by the genome walking on the upstream and the downstream from cdtB gene.

公开(公告)号：CA2697480C

公开(公告)日：2017-07-11

申请号：CA2697480

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  SAMOSORUNSUK WORADA ,  ASAKURA MASAHIRO

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C. hyointestinalis and polynucleotides encoding it, and novel methods for detection of C. hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter-like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni, C. coli, and C. fetus, but not by multiplex PCR that can specifically detect the cdtA, cdtB, and cdtC genes of the three bacterial species. The bacterial strain was identified as C. hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

公开(公告)号：AU2008292375A1

公开(公告)日：2009-03-05

申请号：AU2008292375

申请日：2008-08-29

Applicant: FUSO PHARMACEUTICAL IND ,  UNIV OSAKA PREFECTURE

Inventor： SAMOSORUNSUK WORADA ,  ASAKURA MASAHIRO ,  YAMASAKI SHINJI

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C . hyointestinalis and polynucleotides encoding it, and novel methods for detection of C . hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter -like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni , C. coli, and C . fetus, but not by multiplex PCR that can specifically detect the cdtA , cdtB , and cdtC genes of the three bacterial species. The bacterial strain was identified as C . hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.

公开(公告)号：AU2008292375B2

公开(公告)日：2014-07-03

申请号：AU2008292375

申请日：2008-08-29

Applicant: UNIV OSAKA PREFECTURE ,  FUSO PHARMACEUTICAL IND

Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO ,  SAMOSORUNSUK WORADA

IPC: C12N15/09 ,  C07K14/205 ,  C07K16/12 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/569

Abstract: Disclosed are: a cytolethal distending toxin (CDT) derived from

公开(公告)号：AU2008292375B8

公开(公告)日：2014-08-14

申请号：AU2008292375

申请日：2008-08-29

Applicant: FUSO PHARM IND LTD ,  UNIV OSAKA PREFECTURE

Inventor： YAMASAKI SHINJI ,  ASAKURA MASAHIRO ,  SAMOSORUNSUK WORADA

Abstract: An objective of the present invention is to provide the cytolethal distending toxin (CDT) of C . hyointestinalis and polynucleotides encoding it, and novel methods for detection of C . hyointestinalis using the cdt genes. The present inventors focused on the cytolethal distending toxin (CDT) of Campylobacter bacteria, and detected the cdt genes of a Campylobacter -like bacterium isolated from an enteritis patient in Thailand. The present inventors discovered a bacterial strain whose cdtB gene was amplified by common primers in C. jejuni , C. coli, and C . fetus, but not by multiplex PCR that can specifically detect the cdtA , cdtB , and cdtC genes of the three bacterial species. The bacterial strain was identified as C . hyointestinalis by 16S rRNA gene analysis. Furthermore, the entire nucleotide sequence of the cdt genes was determined by genome walking upstream and downstream of the cdtB gene.