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公开(公告)号：WO2014064132A1
公开(公告)日：2014-05-01
申请号：PCT/EP2013/072128
申请日：2013-10-23
Applicant: UPFRONT CHROMATOGRAPHY A/S
Inventor： LIHME, Allan Otto Fog , HANSEN, Marie Bendix , PONTOPPIDAN, Martin
IPC: C07K1/22 , C07K14/415
CPC classification number: C07K1/22 , A23J1/14 , C07K1/1136 , C07K1/14 , C07K14/415 , C08L89/00
Abstract: The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.
Abstract translation: 本发明提供了分离大豆蛋白的方法。 该方法开始于水提取物或大豆蛋白溶液，其通过至少一个膨胀床吸收（EBA）方法。 EBA方法包括使大豆蛋白质的水提取物或溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质（例如胰蛋白酶抑制剂（TI）蛋白质或β-伴大豆球蛋白））。 本发明还提供可通过本发明的方法获得的各种新型蛋白质组合物。
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2.发明申请ISOLATION AND SEPARATION OF MINIMALLY DENATURED POTATO PROTEINS AND PEPTIDES 审中-公开最小去除的蛋白质和蛋白质的分离和分离
公开(公告)号：WO2008092450A1
公开(公告)日：2008-08-07
申请号：PCT/DK2008/000039
申请日：2008-01-29
Applicant: UPFRONT CHROMATOGRAPHY A/S , LIHME, Allan, Otto, Fog , HANSEN, Marie, Bendix , PONTOPPIDAN, Martin
Inventor： LIHME, Allan, Otto, Fog , HANSEN, Marie, Bendix , PONTOPPIDAN, Martin
IPC: C07K14/81 , C07K14/415
CPC classification number: C07K14/81 , C07K1/22 , C07K14/415
Abstract: The present invention relates to the large-scale fractionation and isolation of peptides, polypeptides and protein(s) from a potato derived protein solution such as potato extract, potato fruit juice and fruit water using an adsorbent coupled with a ligand for the capture of the protein(s), from the protein solution. In particular the invention relates to a process for the isolation and separation of patatin and potato protease inhibitors using a low temperature non-denaturing process.
Abstract translation: 本发明涉及使用与配体偶联的吸附剂从马铃薯衍生的蛋白质溶液如马铃薯提取物，马铃薯果汁和水果水中大量分级分离和分离肽，多肽和蛋白质，以捕获 蛋白质，从蛋白质溶液中。 特别地，本发明涉及使用低温非变性方法分离和分离patatin和马铃薯蛋白酶抑制剂的方法。
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Patent Agency Ranking
公开(公告)号：WO2015114045A1
公开(公告)日：2015-08-06
申请号：PCT/EP2015/051798
申请日：2015-01-29
Applicant: UPFRONT CHROMATOGRAPHY A/S
Inventor： LIHME, Allan, Otto, Fog , HANSEN, Marie, Bendix , PONTOPPIDAN, Martin
CPC classification number: C07K1/22 , A23J1/14 , A23L33/185 , C07K14/42
Abstract: The invention provides a process for the separation of pea protein. The process begins with an aqueous extract or solution of pea protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of pea protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest are isolated by eluting them from said adsorbent resin. The invention also provides various protein compositions obtainable via the method of the invention.
Abstract translation: 本发明提供了分离豌豆蛋白的方法。 该方法开始于水提取物或豌豆蛋白溶液，其通过至少一个扩展床吸收（EBA）方法。 EBA方法包括将水提取物或豌豆蛋白溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质。 本发明还提供可通过本发明的方法获得的各种蛋白质组合物。
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公开(公告)号：EP2912052A1
公开(公告)日：2015-09-02
申请号：EP13792269.6
申请日：2013-10-23
Applicant: UPFRONT CHROMATOGRAPHY A/S
Inventor： LIHME, Allan Otto Fog , HANSEN, Marie Bendix , PONTOPPIDAN, Martin
IPC: C07K1/22 , C07K14/415
CPC classification number: C07K1/22 , A23J1/14 , C07K1/1136 , C07K1/14 , C07K14/415 , C08L89/00
Abstract: The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.
Abstract translation: 本发明提供了分离大豆蛋白的方法。 该方法开始于大豆蛋白质的水提取物或溶液，其通过至少一个扩展床吸收（EBA）方法。 EBA方法包括使大豆蛋白质的水提取物或溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质（例如胰蛋白酶抑制剂（TI）蛋白质或β-伴大豆球蛋白））。 本发明还提供可通过本发明的方法获得的各种新型蛋白质组合物。
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公开(公告)号：EP3099187B1
公开(公告)日：2018-06-06
申请号：EP15701544.7
申请日：2015-01-29
Applicant: UPFRONT CHROMATOGRAPHY A/S
Inventor： LIHME, Allan, Otto, Fog , HANSEN, Marie, Bendix , PONTOPPIDAN, Martin
CPC classification number: C07K1/22 , A23J1/14 , A23L33/185 , C07K14/42
Abstract: The invention provides a process for the separation of pea protein. The process begins with an aqueous extract or solution of pea protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of pea protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest are isolated by eluting them from said adsorbent resin. The invention also provides various protein compositions obtainable via the method of the invention.
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公开(公告)号：EP3099187A1
公开(公告)日：2016-12-07
申请号：EP15701544.7
申请日：2015-01-29
Applicant: UPFRONT CHROMATOGRAPHY A/S
Inventor： LIHME, Allan, Otto, Fog , HANSEN, Marie, Bendix , PONTOPPIDAN, Martin
CPC classification number: C07K1/22 , A23J1/14 , A23L33/185 , C07K14/42
Abstract: The invention provides a process for the separation of pea protein. The process begins with an aqueous extract or solution of pea protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of pea protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest are isolated by eluting them from said adsorbent resin. The invention also provides various protein compositions obtainable via the method of the invention.
Abstract translation: 本发明提供了分离豌豆蛋白的方法。 该方法开始于水提取物或豌豆蛋白质溶液，其通过至少一个膨胀床吸收（EBA）方法。 EBA方法包括将水提取物或豌豆蛋白溶液与至少一种吸附树脂接触，所述吸附树脂包含至少一种具有特定化学结构的配体（L1或L2）。 通过从所述吸附树脂洗脱来分离感兴趣的蛋白质。 本发明还提供可通过本发明的方法获得的各种蛋白质组合物。
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7.发明公开ISOLATION AND SEPARATION OF MINIMALLY DENATURED POTATO PROTEINS AND PEPTIDES 审中-公开隔离和最小的变性马铃薯的蛋白质和肽的纯化
公开(公告)号：EP2115000A1
公开(公告)日：2009-11-11
申请号：EP08700910.6
申请日：2008-01-29
Applicant: UPFRONT CHROMATOGRAPHY A/S
Inventor： LIHME, Allan, Otto, Fog , HANSEN, Marie, Bendix , PONTOPPIDAN, Martin
IPC: C07K14/81 , C07K14/415
CPC classification number: C07K14/81 , C07K1/22 , C07K14/415
Abstract: The present invention relates to the large-scale fractionation and isolation of peptides, polypeptides and protein(s) from a potato derived protein solution such as potato extract, potato fruit juice and fruit water using an adsorbent coupled with a ligand for the capture of the protein(s), from the protein solution. In particular the invention relates to a process for the isolation and separation of patatin and potato protease inhibitors using a low temperature non-denaturing process.
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