Abstract:
본 발명은 인간 배아줄기세포를 결합 조직 성장 인자(connective tissue growth factor, CTGF) 및 소태아혈청(fetal bovine serum, FBS)을 포함하는 배지 중에서 배양하는 것을 포함하는, 인간 배아줄기세포의 중간엽 줄기세포로의 분화방법을 제공한다. 또한, 본 발명은 CTGF 및 FBS를 포함하는, 인간 배아줄기세포의 중간엽 줄기세포로의 분화용 배지를 제공한다. 또한, 본 발명은 인간 배아줄기세포의 중간엽 줄기세포로의 분화용 배지 및 중간엽 줄기세포의 배양용 배지를 제공한다.
Abstract:
PURPOSE: A method for differentiating human embryonic stem cells into mesenchymal stem cells is provided to enhance differentiation efficiency. CONSTITUTION: A method for differentiating human embryonic stem cells into mesenchymal stem cells comprises a step of culturing the human embryonic stem cells in a medium containing 80-120 ng/ml of CTGF(connective tissue growth factor) and 2-5 w/v% of FBS(fetal bovine serum) for 2-5 days with daily fresh medium.
Abstract translation:目的:提供一种将人胚胎干细胞分化为间充质干细胞的方法,以提高分化效率。 构成:将人胚胎干细胞分化为间充质干细胞的方法包括在含有80-120ng / ml CTGF(结缔组织生长因子)和2-5w / v%的培养基中培养人胚胎干细胞的步骤, 的FBS(胎牛血清)2-5天,每日新鲜培养基。
Abstract:
본 발명은 배아줄기세포 유래 혈관형성전구세포 배양액 또는 배양 분비물을 이용한 피부재생용 조성물 및 이의 용도에 관한 것으로, 본 발명은 배아줄기세포 유래 혈관형성전구세포 자체가 아닌 배양 분비물을 치료제로 이용하므로 기형종(teratoma) 형성의 위험성이 없고, 신생혈관 생성능력을 촉진시켜 창상치료, 화상창상치료에 효과적이며, 이를 화장품 원료로 이용하는 경우 콜라겐 생성을 증대시켜 피부노화를 방지하는 효과를 제공한다. 특히, 상기 배양 분비물을 고농도로 농축시킨 본 발명의 조성물은 종래 hGH(human growth hormone)에 비해 높은 창상치료효과를 제공한다.
Abstract:
PURPOSE: An injection needle for cell injection and a syringe including thereof are provided to prevent leaking of cell solution which is injected into cardiac muscles by forming an injection needle shaft in a spiral shape. CONSTITUTION: An injection needle for cell injection(10) includes a spiral shaped needle shaft(11). A plurality of openings is formed on side of the injection needle shaft. The needle shaft includes bioabsorbable material in order to decompose within organs. The needle shaft comprises a second shaft of bioabsorbable material which is made of metal and a first shaft. The syringe comprises an injection needle having a spiral shape, an injector barrel which has an injection needle, and a plunger which is inserted into the injector barrel.
Abstract:
본 발명은 (a)이식을 위한 배아 줄기세포 유래의 혈관전구세포를 배양하는 단계; (b)상기 배양된 배아 줄기세포 유래 혈관전구세포를 포유동물의 피부에 이식하는 단계를 포함하는, 배아 줄기세포 유래 혈관전구세포를 이용한 창상 치료 방법을 제공한다. 본 발명의 창상 치료 방법은 기존 창상 치료에 효과가 있다고 알려진 혈관전구세포의 새로운 세포 공급원인 인간배아 줄기세포 유래 혈관전구세포를 이용함으로써, 세포 공급원에 제한성을 없앴으며, 제대혈 줄기세포 유래 혈관전구세포 등 종래의 성체 줄기세포 유래 혈관전구세포보다 창상 치료 효율을 크게 향상 시키는 효과가 있다. 또한, 본 발명의 창상 치료 방법에 따르면 이식된 인간배아 줄기세포 유래 혈관전구세포는 혈관 신생을 촉진시켜 상처 치유에 필요한 인자들의 전이를 용이하게 하여, 상처 치유에 필요한 콜라겐의 합성을 증가시켰으며, 새로이 형성된 콜라겐층이 상처 치유를 위해 필요한 기질 세포 및 피부 세포 등이 보다 용이하게 자랄 수 있는 환경을 조성하여, 종래 기술보다 상처 치유 기간을 단축시키는 효과가 있다. 인간배아 줄기세포, 창상 치료, 인간배아 줄기세포 유래 혈관전구세포
Abstract:
PURPOSE: A cosmetic composition containing embryonic stem cell-derived vascular angiogenic progenitor cell culture or fraction thereof is provided to suppress tyrosinase activity and to ensure excellent skin whitening. CONSTITUTION: A cosmetic composition for skin whitening contains embryonic stem cell-derived vascular angiogenic progenitor cell culture or fraction thereof as an active ingredient. The culture is prepared by a culture, centrifuging embryonic stem cell-derived vascular angiogenic progenitor cells culture medium to obtain supernatant, concentrate of the supernatant, or freeze-dried product of the supernatant. The embryonic stem cell is human embryonic stem cells.
Abstract:
PURPOSE: A method for diedifferentiating cells using a nano-delivery system is provided to improve safety using a virus vector and to supply a large mount of proteins. CONSTITUTION: A method for dedifferentiating cells comprises: a step of binding a dedifferentiation transcription factor to a nanotube having a silicon dioxide layer to prepare a transcription factor-nanotube delivery system; a step of culturing the transcription-nanotube delivery system and cells; and a step of isolating embryonic stem cell-like colony.
Abstract:
PURPOSE: A composition containing diterpene compound with kauran skeleton is provide to enhance proliferation ability of skin stem cells and epithermal keratinocytes and to prevent or treat skin injury. CONSTITUTION: A pharmaceutical composition for preventing or treating skin injury due to UV ray contains Siegesbeckia extract as an active ingredient. The skin injury is burn, erythema, or pigmentation on epidermis or dermis. The Siegesbeckia extract is obtained using water, alcohol of C1-C4, or mixture thereof. A cosmetic composition for treating skin injury due to UV ray contains the Siegesbeckia extract as an active ingredient. A pharmaceutical or cosmetic composition for preventing or treating skin injury due to UV ray contains a compound of chemical formula 1.
Abstract:
PURPOSE: A composition containing embryonic stem cell-derived endothelial progenitor cell culture liquid or culture secretion is provided to use as a therapeutic agent and to use in wound healing. CONSTITUTION: A composition for skin regeneration contains culture secretion of embryonic stem cell-derived endothelial progenitor cell or fraction thereof as an active ingredient. The embryonic stem cells are human embryonic stem cells. The composition is used in wound healing or skin plastic surgery. The composition contains 1,250-1,250,000 pg/ml of epidermal growth factor(EFG), 35-35,000 pg/ml of basic fibroblast growth factor(FGF-2), 650-650,000 pg/ml of platelet-derived growth factor-AA(PDGF-AA), and 400-400,000 pg/ml of vascular endothelial growth factor(VEGF) as active ingredients.