公开(公告)号：KR1020090123162A

公开(公告)日：2009-12-02

申请号：KR1020080049104

申请日：2008-05-27

Applicant: 강릉원주대학교산학협력단

Inventor： 어명희 ,  김옥선 ,  조순영 ,  김가현 ,  이광호

IPC: C12P23/00

CPC classification number: C12P23/00 ,  C12N1/20

Abstract: PURPOSE: A method for culturing zeaxanthin producing strain is provided to massively zeaxanthin with high yield from marine biomass regardless of season. CONSTITUTION: A method for culturing zeaxanthin producing strain comprises: a first step of isolating strain having productivity of zeaxanthin; a second step of sub-culturing isolated strain; a third step of measuring productivity of zeaxanthin; and a step of adding medium at 1~50°C and 10~300 rpm then culturing. The strain having productivity of zeaxanthin is Micrococcus luteus, Vibrio, Pseudomonas, Achromobacter, Aeromonas, Photobacterium, Acetobacter, Gluconobacter, Alcaligenes, or Flavobacterium.

Abstract translation: 目的：提供玉米黄质生产菌株的培养方法，无论季节如何，都可以大量生产来自海洋生物质的玉米黄质。 构成：培养玉米黄质生产菌株的方法包括：分离具有玉米黄质生产力的菌株的第一步骤; 亚培养分离菌株的第二步; 测量玉米黄质生产力的第三步; 并在1〜50℃，10〜300rpm下加入培养基，然后培养。 具有玉米黄质生产力的菌株是藤黄微球菌，弧菌属，假单胞菌属，无色杆菌属，气单胞菌属，光杆菌属，醋杆菌属，葡糖杆菌属，产碱杆菌属或黄杆菌属。

公开(公告)号：KR1020090090943A

公开(公告)日：2009-08-26

申请号：KR1020080016519

申请日：2008-02-22

Applicant: 강릉원주대학교산학협력단

Inventor： 김광우 ,  김가현 ,  어명희 ,  김옥선 ,  조순영

IPC: A23K1/10 ,  A23K1/18 ,  A23K1/00

CPC classification number: A23K10/22 ,  A23K20/174 ,  A23K20/30 ,  A23K50/80

Abstract: A manufacturing method of a feed fish using protein source as a substitute for fish meal separated from liquid waste from mackerel processing is provided to add the water-soluble protein collected from the mackerel edge processing waste water. A manufacturing method of a fish farm fodder comprises next steps. The hydrochloric acid solution of about 0.01-10N is added to the mackerels processing waste water. The pH of the processing waste water is controlled as the fixed value between about 4-7 and centrifuges about 1-100 degrees and the mackerels extracts is collected. The collected mackerels extract the fish meal, wheat flour, vitamin mixture, mineral mixture, salt, yeast and fish tallow. The mixture is molded by a pelletizer, dries with the cold air drying during 1-10 days and pulverizes to the proper size. The feed for fish farming is comprised of 2-30wt% mackerels extracts, 20-40wt% fish meal, 18-75wt% wheat flour, 0.3-2wt% vitamin mixture, 0.3-2wt% mineral mixture, 0.3-2wt% salt, 0.1-1wt% yeast and 2-5wt% fish tallow.

Abstract translation: 提供使用蛋白质源作为从鲭鱼加工液体废物中分离的鱼粉的替代物的饲料的制造方法，以加入从鲭鱼边缘处理废水中收集的水溶性蛋白质。 鱼饲料的制造方法包括以下步骤。 将约0.01-10N的盐酸溶液加入到鲭鱼加工废水中。 处理废水的pH值控制在约4-7之间的固定值，离心约1-100度，并收集鲭鱼提取物。 收集的鲭鱼提取鱼粉，小麦粉，维生素混合物，矿物混合物，盐，酵母和鱼脂。 混合物用造粒机成型，在1-10天内用冷空气干燥并干燥至适当的大小。 养鱼饲料由2-30重量％鲭鱼提取物，20-40重量％鱼粉，18-75重量％小麦粉，0.3-2重量％维生素混合物，0.3-2重量％矿物混合物，0.3-2重量％盐，0.1 -1wt％酵母和2-5wt％鱼油。

公开(公告)号：KR1020090092897A

公开(公告)日：2009-09-02

申请号：KR1020080018122

申请日：2008-02-28

Applicant: 강릉원주대학교산학협력단

Inventor： 김가현 ,  어명희 ,  김옥선 ,  조순영

IPC: C12N1/20 ,  C12N1/00

CPC classification number: C12N1/20 ,  C12N2523/00 ,  C12P7/6427

Abstract: A method for culturing microorganism which produces EPA (eicosapentaenoic acid) is provided to ensure optical condition of culturing in culture temperature, stirring rate, and NaCl concentration. A method for culturing microorganism which produces EPA comprises: a first step of isolating a strain having EPA productivity; a second step of sub-culturing strain and cooling at 1~20°C; a third step of measuring and identifying EPA productivity of strain; and a fourth step of adding media at 1~50 °C and 10~300 rpm and culturing.

Abstract translation: 提供一种培养产生EPA（二十碳五烯酸）的微生物的方法，以确保在培养温度，搅拌速率和NaCl浓度下培养的光学条件。 生产EPA的培养微生物的方法包括：分离具有EPA生产力的菌株的第一步骤; 在1〜20℃下亚菌培养和冷却的第二步; 测量和鉴定菌株的EPA生产力的第三步; 第4步，在1〜50℃，10〜300转/分的条件下加入培养基，培养。

公开(公告)号：KR1020090090945A

公开(公告)日：2009-08-26

申请号：KR1020080016522

申请日：2008-02-22

Applicant: 강릉원주대학교산학협력단

Inventor： 김광우 ,  김가현 ,  어명희 ,  김옥선 ,  조순영

IPC: A23K1/10 ,  A23K1/18 ,  A23K1/00 ,  A23K1/16

CPC classification number: A23K10/26 ,  A23K10/22 ,  A23K50/80

Abstract: A composition of a feed for fish farming separated from liquid waste from mackerel processing capable of using a protein source as a substitute for fish meal is provided to replace the fish meal which is the protein source of the feed for fish farming. A composition of a feed for fish farming is separated from the mackerels processing waste water and includes the water-soluble protein usable as fish meal substitution protein. The mackerels processing waste water is the waste water coming from the mackerels processing plant. The water-soluble protein is separated from the mackerels processing waste water through isoelectric precipitation. The water-soluble protein is added so that it become 30 percent of the total fish meal. The mackerels comprises one or more mackerelses selected from the group of the Scomber japonicus and Scomber australasicus.

Abstract translation: 提供了一种从液体废物中分离出的养鱼饲料的组合物，可以使用蛋白质来代替鱼粉，以代替作为鱼类养殖饲料蛋白质来源的鱼粉。 用于养鱼的饲料的组合物与鲭鱼加工废水分离，并且包括可用作鱼粉替代蛋白质的水溶性蛋白质。 处理废水的鲭鱼是鲭鱼加工厂的废水。 水溶性蛋白质通过等电沉淀从鲭鱼加工废水中分离出来。 添加水溶性蛋白质，使其成为总鱼粉的30％。 鲭鱼包含从Scomber japonicus和Scomber australasicus组中选出的一种或多种鲭鱼。