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公开(公告)号：KR1020120119061A
公开(公告)日：2012-10-30
申请号：KR1020110036802
申请日：2011-04-20
Applicant: 강원대학교산학협력단
IPC: C07K16/18 , C12N15/13 , A61K39/395 , A61P35/00
Abstract: PURPOSE: An antibody for n-terminus protein of PRDM 10 and a manufacturing method thereof are provided to be usefully used in the PRDM 10 protein function research. CONSTITUTION: An antibody for n-terminus protein of PRDM 10 is described in the sequence number 1(SEQ ID NO:1). The gene which codes the N- terminus protein has the base sequence described in the sequence number 2. The N- terminus protein of the PRDM 10 includes the zinc finger motif. A composition for detecting the PRDM 10 protein includes the antibody which unites with the N- terminus protein of the PRDM 10. The PRDM 10 related disease is inflammatory arthritis or cancer. A manufacturing method of the antibody for N terminus protein of the PRDM 10 comprises the following steps: processing PCR by using a primer in order to amplify the N terminous part of the PRDM 10 by using the PRDM 10 as a mold; ligating the amplified N terminous gene of the PRDM 10 and transforming into a host cell to over express the N-terminous protein of the RPDM 10; and obtaining the antibody for N-terminous protein of the RPDM 10 bt injecting the over expressed N terminus protein of the PRDM 10 into animals. [Reference numerals] (AA) Cloning expression using His tagging system; (BB) Antigen protein purification (IMAC); (CC) Immunization reaction induction inside rabbit body
Abstract translation: 目的：提供PRDM 10的n末端蛋白的抗体及其制备方法，可用于PRDM 10蛋白功能研究。 构成：在序列号1（SEQ ID NO：1）中描述了PRDM 10的n末端蛋白的抗体。 编码N-末端蛋白的基因具有序列号2中描述的碱基序列.PRDM 10的N-末端蛋白包括锌指基序。 用于检测PRDM 10蛋白的组合物包括与PRDM 10的N-末端蛋白相结合的抗体.PCR10相关疾病是炎性关节炎或癌症。 PRDM 10的N末端蛋白的抗体的制造方法包括以下步骤：通过使用底漆处理PCR，以便通过使用PRDM 10作为模具来扩增PRDM 10的N末端部分; 连接PRDM 10的扩增的N末端基因并转化到宿主细胞中以过表达RPDM 10的N末端蛋白; 并获得RPDM10b的N末端蛋白的抗体，将PRDM 10的过表达的N末端蛋白质注入动物体内。 （附图标记）（AA）使用His标签系统的克隆表达; （BB）抗原蛋白纯化（IMAC）; （CC）兔体内免疫反应诱导
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公开(公告)号：KR1020120119049A
公开(公告)日：2012-10-30
申请号：KR1020110036783
申请日：2011-04-20
Applicant: 강원대학교산학협력단
IPC: C07K16/18 , C12N15/13 , A61K39/395 , A61P29/00
Abstract: PURPOSE: An antibody for C-terminus part protein of PRDM 10 and a manufacturing method thereof are provided to be usefully used for PRDM 10 protein function research. CONSTITUTION: An antibody combined with the C-terminus part protein of PRDM 10 has the sequence number 1(SEQ ID NO:1). The gene which codes the C- terminus protein has the base sequence described in the sequence number 2. The C- terminus protein of the PRDM 10 includes zinc finger motif. A composition for detecting PRDM 10 protein includes the antibody which unites with the C- terminus protein of the PRDM 10. A pharmaceutical composition for treating or preventing PRDM 10 protein related disorder includes PRDM 10 protein includes the antibody which unites with the C- terminus protein of the PRDM 10 and pharmaceutically acceptable carrier. [Reference numerals] (AA) Cloning expression using His tagging system; (BB) Antigen protein purification (IMAC); (CC) Immune reaction induction in vivo of Rabbit
Abstract translation: 目的：提供PRDM 10的C末端部分蛋白的抗体及其制备方法，可用于PRDM 10蛋白质功能研究。 构成：与PRDM 10的C末端部分蛋白结合的抗体具有序列号1（SEQ ID NO：1）。 编码C-末端蛋白的基因具有序列号2中描述的碱基序列.PRDM 10的C-末端蛋白包括锌指基序。 用于检测PRDM 10蛋白的组合物包括与PRDM 10的C-末端蛋白相结合的抗体。用于治疗或预防PRDM 10蛋白相关病症的药物组合物包括PRDM 10蛋白质包括与C-末端蛋白质结合的抗体 的PRDM 10和药学上可接受的载体。 （附图标记）（AA）使用His标签系统的克隆表达; （BB）抗原蛋白纯化（IMAC）; （CC）兔体内免疫反应诱导
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