公开(公告)号：KR100697610B1

公开(公告)日：2007-03-22

申请号：KR1020050115490

申请日：2005-11-30

Applicant: 강원대학교산학협력단

Inventor： 이현용 ,  김정화 ,  김대호 ,  김철희 ,  김효성 ,  권민철 ,  김금용

IPC: C12N1/12 ,  C12M1/00

Abstract: A spirulina extract is provided to obtain the cultivated spirulina extract having cancer cell growth inhibition effect and human immuno-cell growth promotion activity by culturing spirulina using highly purified deep water. The spirulina extract is prepared by hot water-extracting a spirulina sample cultivated by the spirulina culturing method by adding solvent of ten times of the amount of the spirulina sample thereto, filtering and concentrating it under reduced pressure and then freeze-drying it. The method for culturing the spirulina comprises the steps of: (a) storing deep water at a temperature of 4 deg.C for 7 days with removing precipitated sediment therefrom; (b) passing the deep water through a 0.2 micrometer membrane filter to obtain highly pure deep water; (c) adding 30-50% of the highly purified deep water into a spirulina culture solution; and (d) culturing the spirulina at a temperature of 30-50 deg.C for 15 days.

Abstract translation: 提供螺旋藻提取物以通过使用高度纯化的深水培养螺旋藻来获得具有癌细胞生长抑制作用和人免疫细胞生长促进活性的培养螺旋藻提取物。 螺旋藻提取物是通过用螺旋藻培养法培养的螺旋藻样品热水提取，加入十倍量的螺旋藻样品的溶剂，过滤并浓缩，然后冷冻干燥。 用于培养螺旋藻的方法包括以下步骤：（a）在4℃的温度下将深水储存7天，从其中除去沉淀的沉淀物; （b）将深水通过0.2微米膜过滤器，以获得高纯度的深水; （c）将30-50％的高纯度深水加入到螺旋藻培养液中; 和（d）在30-50℃的温度下培养螺旋藻15天。