-
Patent Agency Ranking
公开(公告)号：KR100670612B1
公开(公告)日：2007-01-17
申请号：KR1020050083777
申请日：2005-09-08
Applicant: 강원대학교산학협력단
IPC: A61K36/254 , A61K36/232 , A61K36/73
Abstract: A method for extracting herbs is provided to be able to obtain an herb extract containing many useful ingredients, and improve the extraction yield compared to other extraction method by performing a hot-water extraction, an ultrasonic wave extraction and a high hydraulic extraction in complex. The method comprises the steps of: (a) after adding solvent, the amount of which is 10 times that of herb, into the herb, hot-water extracting it at a temperature of 40-60 deg.C for 24 hours; (b) after extracting it obtained from the step(a) for 30 minutes using ultrasonic wave having a frequency of 20-40 kHz, freezing the extract under a temperature of -20 deg.C and crushing it; and (c) after extracting it obtained from the step(b) for 1-5 minutes under the pressure of 200-300 mPa using a high hydraulic extractor, filtering under reduced pressure, concentrating and freeze-drying it.
Abstract translation: 提供了一种提取草药的方法，通过进行热水提取，超声波提取和复合物中的高液相萃取，可以获得含有许多有用成分的草本提取物，并提高其提取率。 该方法包括以下步骤：（a）将加入量为草药10倍的溶剂加入草药中，将热水在40-60℃的温度下提取24小时; （b）使用频率为20〜40kHz的超声波从步骤（a）中提取30分钟后，在-20℃的温度下冷冻该提取液并进行粉碎; 和（c）使用高液压萃取器在200-300mPa的压力下将其从步骤（b）中提取得到1-5分钟后，在减压下过滤，浓缩并冷冻干燥。
-
公开(公告)号：KR100697610B1
公开(公告)日：2007-03-22
申请号：KR1020050115490
申请日：2005-11-30
Applicant: 강원대학교산학협력단
Abstract: A spirulina extract is provided to obtain the cultivated spirulina extract having cancer cell growth inhibition effect and human immuno-cell growth promotion activity by culturing spirulina using highly purified deep water. The spirulina extract is prepared by hot water-extracting a spirulina sample cultivated by the spirulina culturing method by adding solvent of ten times of the amount of the spirulina sample thereto, filtering and concentrating it under reduced pressure and then freeze-drying it. The method for culturing the spirulina comprises the steps of: (a) storing deep water at a temperature of 4 deg.C for 7 days with removing precipitated sediment therefrom; (b) passing the deep water through a 0.2 micrometer membrane filter to obtain highly pure deep water; (c) adding 30-50% of the highly purified deep water into a spirulina culture solution; and (d) culturing the spirulina at a temperature of 30-50 deg.C for 15 days.
Abstract translation: 提供螺旋藻提取物以通过使用高度纯化的深水培养螺旋藻来获得具有癌细胞生长抑制作用和人免疫细胞生长促进活性的培养螺旋藻提取物。 螺旋藻提取物是通过用螺旋藻培养法培养的螺旋藻样品热水提取，加入十倍量的螺旋藻样品的溶剂，过滤并浓缩，然后冷冻干燥。 用于培养螺旋藻的方法包括以下步骤：（a）在4℃的温度下将深水储存7天，从其中除去沉淀的沉淀物; （b）将深水通过0.2微米膜过滤器，以获得高纯度的深水; （c）将30-50％的高纯度深水加入到螺旋藻培养液中; 和（d）在30-50℃的温度下培养螺旋藻15天。
-
公开(公告)号：KR1020080037805A
公开(公告)日：2008-05-02
申请号：KR1020060104909
申请日：2006-10-27
Applicant: 강원대학교산학협력단
CPC classification number: A23L2/04 , A23L2/38 , A23V2002/00 , A23V2200/324 , A23V2250/21
Abstract: Rubus fruit extract having increased immune activity through ultrasonic extraction at low temperature and a fractionation stage is provided to exhibit higher immune activity and increased cell differentiation degree by an increase of differentiation-inducing material through multi-step differentiation. Rubus fruit is extracted in distilled water at 60deg.C for 24hr, ultrasonically extracted at 40KHz for 30min and fractionated to chloroform, ethyl acetate and butanol in order. For an example, Rubus fruit is washed, dried at 25 to 30deg.C in a shade and ground to 150meshes. The Rubus fruit powder is extracted in water at 100deg.C for 12hr, ultrasonically extracted at 40 KHz for 30min, filtered and concentrated under reduced pressure. The Rubus fruit concentrates are fractionated in steps containing chloroform, ethyl acetate and butanol in order, concentrated under reduced pressure and vacuum-dried.
Abstract translation: 通过在低温和分级分离阶段通过超声提取具有增加的免疫活性的鲁氏果实提取物通过多步分化增加分化诱导材料以显示更高的免疫活性和增加的细胞分化程度。 悬浮果实在60℃的蒸馏水中提取24小时，在40KHz下超声提取30分钟，分级分为氯仿，乙酸乙酯和丁醇。 例如，将胡子水果洗涤，在阴凉处25至30℃干燥并研磨至150目。 将芦荟果粉在100℃水中提取12小时，以40KHz超声提取30分钟，过滤并减压浓缩。 将Rubus果实浓缩物按步骤分批分级，依次加入氯仿，乙酸乙酯和丁醇，减压浓缩并真空干燥。
-
公开(公告)号：KR100655798B1
公开(公告)日：2006-12-11
申请号：KR1020050124089
申请日：2005-12-15
Applicant: 강원대학교산학협력단 , 강화군
IPC: A61K36/282 , A61P1/16
Abstract: A method for extracting mugwort is provided to improve the liver detoxication capacity of a mugwort extract by using a mixture extract consisting of water and ethanol in the ratio of 70:30 for low-temperature extraction of the mugwort. The method comprises the steps of: (a) after dipping mugwort from Ganghwa in a mixture solvent, ten times of the weight of the mugwort, prepared by mixing water with ethanol in the ratio of 70:30 using an extraction flask at a temperature of 20-25 deg.C for 2 hours, performing a primary low-temperature extraction thereon at a temperature of 40-45 deg.C for 12 hours using an extraction flask having a vertical reflux condenser; and (b) after dipping the first extract in the solvent at a temperature of 20-25 deg.C for 2 hours, performing a secondary low-temperature extraction thereon at a temperature of 50-55 deg.C for 12 hours using an extraction flask having a vertical reflux condenser.
Abstract translation: 提供艾蒿提取方法以通过使用由水和乙醇组成的混合物提取物以70:30的比率用于艾蒿的低温提取来改善艾蒿提取物的肝脏解毒能力。 所述方法包括以下步骤：（a）在混合溶剂中浸泡来自江华的艾蒿之后，使用提取烧瓶在70:30的温度下将水与乙醇混合制备的艾蒿重量的10倍 20-25℃2小时，使用具有垂直回流冷凝器的萃取瓶在40-45℃的温度下对其进行初步低温萃取12小时; （b）在20-25℃的温度下将第一提取物浸渍在溶剂中2小时后，在50-55℃的温度下使用提取在其上进行二次低温提取12小时 具有垂直回流冷凝器的烧瓶。
-
公开(公告)号：KR1020060037048A
公开(公告)日：2006-05-03
申请号：KR1020040086168
申请日：2004-10-27
Applicant: 강원대학교산학협력단
CPC classification number: A23L33/105 , A23L3/30
Abstract: 본 발명은 저온 초음파 병행 추출을 이용 지금까지 면역 및 항암활성 등에 생리활성이 뛰어나다고 알려져 있던 한국산 생약재를 이용하여 기존의 단일 열수 추출방법과 초음파 단일 추출이 아닌 추출공정 중 초음파를 병행 저온에서 추출하였을 때의 생약재의 면역 활성 증진 효과에 대한 것이다.
이를 위해 본 발명은 생약재 추출을 면역 활성 증진 효과를 위하여 기존의 고온인 100℃나 아주 낮은 온도인 40℃에서의 추출이 아닌 60℃의 저온에서 시료의 10배수의 용매를 첨가하여 1차로 추출한 후, 2차로 40 kHz의 초음파를 병행 처리한 후에, 감압여과?농축 동결 건조하여 추출물을 제조하는 추출 방법을 사용하였다.
기존의 단일 열수 추출방법으로 추출시 100℃에서는 열에 의한 추출물의 변성이나 열손실과 열에 의한 성분의 파괴 등의 단점이 나타났고, 40℃의 낮은 온도에서의 추출은 활성이 낮게 나타났고, 또한 수율면이나 성분의 추출이 미쳐다 이루어지지 않았으며, 초음파 단일 처리시 열에 의하여 추출되어지는 성분의 부족이나 초음파 자체의 의한 열로 인하여 열파괴를 가져왔던 것에 비하여 저온 초음파 병행 추출은 열에 의한 성분의 파괴, 변성, 손실 등이 가장 적게 나타났고, 2배 이상의 수율 증진 효과를 보였으며, 면역 세포의 생육 활성 증진에 있어서는 30%이상의 활성 증진을 나타내었으며, 다양한 면역 활성 증진 실험에서도 마찬가지로 저온 초음파 병행 추출물이 1.3～1.8배의 증진 효과를 나타났다. 기존의 단일 열수 추출방법 과 초음파 단일 처리 보다는 저온 초음파 병행 추출법을 이용하는 것이 생약재의 면역 활성 증진에 있어서 더 뛰어났다.
초음파처리, 감압여과, 농축, 동결건조
-
-
-
-
Search Results
5
records
(took 0.017 seconds)
