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公开(公告)号：KR1020130048416A
公开(公告)日：2013-05-10
申请号：KR1020110113256
申请日：2011-11-02
Applicant: 강원대학교산학협력단
Abstract: PURPOSE: A medium for producing sepiapterin containing a rice protein and a method for producing sepiapterin using the same are provided to obtain high yield and high productivity sepiapterin using rice protein hydrolysate as a nitrogen source. CONSTITUTION: A medium for producing sepiapterin contains a rice protein or a rice protein hydrolysate. The rice protein or the rice protein hydrolysate is added as a nitrogen source. A method for producing the rice protein comprises: a step of pulverizing rice; a step of adding a lipid soluble material-extracting solvent and suspending and removing lipid; a step of adding an alkaline solution to the rice and adjusting to pH 9.0-9.5; a step of centrifuging to remove insoluble materials and collecting a primary supernatant; a step of adding an acidic solution to the primary supernatant and adjusting to pH 4.0-4.5; a step of centrifuging, removing the supernatant, and collecting a primary precipitate; a step of adding distilled water to the primary precipitate and freeze-drying. A method for producing sepiapterin comprises a step of culturing a transformed microorganism in the medium. The microorganism is E.coli. [Reference numerals] (AA) Rice; (BB) Pulverize(120 mesh); (CC) Extract lipid(n-hexane, 3 times); (DD) Dry(12 hr); (EE) Extract protein(pH 9.5); (FF,II,LL) Centrifuge(12,000rpm, 10min); (GG) Supernatant; (HH) Precipitate protein(pH 4.5); (JJ) Precipitate; (KK) Wash(pH 4.5); (MM) Neutralize(pH 7.0); (NN) Freeze-dry; (OO) Protein powder
Abstract translation: 目的：提供含有大米蛋白的sepiapterin的培养基和使用该蛋白的sepiapterin的制备方法，以使用稻蛋白水解产物作为氮源获得高产率和高生产率的sepiapterin。 构成：用于生产sepiapterin的培养基含有大米蛋白或大米蛋白水解产物。 加入米蛋白或大米蛋白水解物作为氮源。 一种水稻蛋白质的制造方法，其特征在于，包括以下步骤： 添加脂溶性物质提取溶剂并悬浮和除去脂质的步骤; 向大米中加入碱性溶液并调节至pH 9.0-9.5的步骤; 离心除去不溶物并收集初级上清液的步骤; 将酸性溶液加入到初级上清液中并调节至pH 4.0-4.5的步骤; 离心，除去上清液并收集初级沉淀物的步骤; 向蒸馏水中加入蒸馏水并冷冻干燥的步骤。 制备sepiapterin的方法包括在培养基中培养转化的微生物的步骤。 微生物是大肠杆菌。 （附图标记）（AA）稻; （BB）粉碎（120目）; （CC）提取脂质（正己烷，3次）; （DD）干燥（12小时）; （EE）提取物蛋白质（pH 9.5）; （FF，II，LL）离心机（12,000rpm，10min）; （GG）上清液; （HH）沉淀蛋白（pH 4.5）; （JJ）沉淀物; （KK）洗涤（pH 4.5）; （MM）中和（pH7.0）; （NN）冷冻干燥; （OO）蛋白粉
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Patent Agency Ranking
公开(公告)号：KR101229912B1
公开(公告)日：2013-02-05
申请号：KR1020110031891
申请日：2011-04-06
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 재조합 대장균을 이용한 세피아프테린의 제조방법에 관한 것으로, GTP 사이클로하이드로라아제(cyclohydrolase) 1 및 6-파이루보일테트라하이드로프테린 신타아제(pyruvoyltetrahydropterin synthase)를 발현하도록 형질전환된 재조합 대장균에, GMP 키나아제(kinase) 및 뉴클레오사이드 다이포스페이트 키나아제(nucleoside diphosphate kinase)을 과발현하도록 추가로 형질전환하면, 대조군에 비해 약 4배 높은 수율로 세피아프테린을 생산할 수 있다.
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公开(公告)号：KR1020120114105A
公开(公告)日：2012-10-16
申请号：KR1020110031891
申请日：2011-04-06
Applicant: 강원대학교산학협력단
Abstract: PURPOSE: A method for preparing sepiapterin using recombinant E.coli is provided to overexpress GMP kinase and nucleoside diphosphate kinase and to produce sepiapterin of high yield. CONSTITUTION: A method for preparing sepiapterin comprises a step of culturing recombinant E.coli. The recombinant E.coli is transformed to express GTP cyclohydrolase 1 and 6-pyruvoyltetrahydropterin synthase. E.coli is transformed by inserting genes encoding GTP cyclohydrolase 1 and 6-pyruvoyltetrahydropterin synthase into a vector and inserting the vector into E.coli.
Abstract translation: 目的：使用重组大肠杆菌制备sepiapterin的方法，用于过表达GMP激酶和核苷二磷酸激酶，并产生高产率的sepiapterin。 构成：制备sepiapterin的方法包括培养重组大肠杆菌的步骤。 转化重组大肠杆菌以表达GTP环化水解酶1和6-丙酮酰四氢蝶呤合酶。 通过将编码GTP环化水解酶1和6-丙酮酰四氢叶酸合酶的基因插入载体并将载体插入大肠杆菌中来转化大肠杆菌。
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公开(公告)号：KR1020130048417A
公开(公告)日：2013-05-10
申请号：KR1020110113257
申请日：2011-11-02
Applicant: 강원대학교산학협력단
IPC: A23L3/3472 , A01N61/00
CPC classification number: A23L3/3472 , A01N61/00 , A23L33/105
Abstract: PURPOSE: A food preservative is provided to facilitate a use as a food preservative by effectively hindering the growth of meat processing food rancid microorganism. CONSTITUTION: A food preservative comprises an agrimony extract as an active ingredient. The agrimony extract uses agrimony as a pumping material, and the agrimony extract is extracted using water having an alkaline acidity and an ethanol aqueous solution as an extraction solvent. The alkaline acidity has a pH of 10-12. Food is a meat processed food. A composition for suppressing the growth of Weissella viridescens includes an agrimony extract as an active ingredient. A composition for suppressing the growth of Leuconostoc carnosum includes an agrimony extract as an active ingredient. [Reference numerals] (AA) Control group; (BB) Water; (CC) 50% ethanol; (DD) Water + ultrasound wave; (EE) 50% ethanol + ultrasound wave
Abstract translation: 目的：提供食品防腐剂，有效阻碍肉类加工食品腐败微生物生长，促进食品防腐剂的使用。 构成：食品防腐剂包括作为有效成分的龙舌兰提取物。 龙舌兰提取物使用龙涎香作为泵送材料，并且使用具有碱性酸度的水和乙醇水溶液作为提取溶剂提取香椿提取物。 碱性酸度的pH值为10-12。 食物是肉类加工食品。 用于抑制薇素氏菌（Weissella viridescens）生长的组合物包括作为有效成分的龙舌兰提取物。 用于抑制明目面肠粘膜生长的组合物包括作为活性成分的龙舌兰提取物。 （附图标记）（AA）对照组; （BB）水; （CC）50％的乙醇; （DD）水+超声波; （EE）50％乙醇+超声波
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5.发明授权
公开(公告)号：KR101324212B1
公开(公告)日：2013-11-06
申请号：KR1020110113256
申请日：2011-11-02
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 쌀 단백질 또는 쌀 단백질의 가수분해물을 포함하는 세피아프테린 생산용 배지 및 이를 이용한 세피아프테린의 생산방법에 관한 것으로, 본 발명의 쌀 단백질 가수분해물을 미생물 배지에 질소원으로 첨가할 경우, 기존의 다른 질소원에 비해 세피아프테린(sepiapterin)을 고 수율 및 고 생산성으로 생산할 수 있다.
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公开(公告)号：KR101324021B1
公开(公告)日：2013-10-31
申请号：KR1020110113257
申请日：2011-11-02
Applicant: 강원대학교산학협력단
IPC: A23L3/3472 , A01N61/00
Abstract: 본 발명은 용아초 추출물을 포함하는 식품 보존제에 관한 것으로, 알카리성 산도의 추출용매를 사용하여 추출한 본 발명의 용아초 추출물은 육가공 식품 변패 미생물의 생육을 효과적으로 저해한다.
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