公开(公告)号：KR100851063B1

公开(公告)日：2008-08-12

申请号：KR1020070007760

申请日：2007-01-25

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  홍수영 ,  조계만

IPC: A23L1/238 ,  A23L1/202 ,  A23L1/28

Abstract: 본 발명은 한국 전통 간장으로부터 분리한 바실러스 퍼밀러스 HY1균주(
Bacillus pumilus HY1 )를 동정하고 상기 균주로부터 생성되는 설펙틴의 분리 및 정제 방법, 상기 균주로부터 분리된 설펙틴의 유방암과 대장암에 대한 세포독성 시험 및 바실러스 퍼밀러스 (
Bacillus

pumilus ) HY1를 이용하여 설펙틴이 다량 함유된 청국장 및 간장의 속성 제조방법을 제공하는 뛰어난 효과가 있다.
바실러스 퍼밀러스, 설펙틴, 청국장, 간장 속성제조법

公开(公告)号：KR100729938B1

公开(公告)日：2007-06-19

申请号：KR1020060078126

申请日：2006-08-18

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  홍수영

IPC: C12N9/24 ,  C12N9/00

Abstract: A novel multifunctional gene cel44C isolated from a Paenibacillus polymyxa GS01 strain is provided to decompose the cell wall of a plant and simultaneously decompose cellulose, xylan, lichenan and mannan, so that the gene is useful for paper industry and organic food production. The novel multifunctional gene cel44C isolated from a Paenibacillus polymyxa GS01 strain has the nucleotide sequence of SEQ ID NO:1, wherein the multifunctional enzyme encoded by the gene cel44C has the amino acid sequence of SEQ ID NO:2; the Paenibacillus polymyxa GS01 strain is isolated from the root of ginseng and is capable of decomposing plant cell wall; and the gene cel44C is analyzed by performing PCR(polymerase chain reaction) with a primer having any one nucleotide sequence selected from SEQ ID NO:3 to SEQ ID NO:8.

Abstract translation: 提供了一种从多粘类芽孢杆菌GS01菌株中分离出的新型多功能基因cel44C，用于分解植物的细胞壁并同时分解纤维素，木聚糖，地衣多糖和甘露聚糖，使得该基因可用于造纸工业和有机食品生产。 从多粘类芽孢杆菌GS01菌株中分离的新型多功能基因cel44C具有SEQ ID NO：1的核苷酸序列，其中由基因cel44C编码的多功能酶具有SEQ ID NO：2的氨基酸序列; 多粘类芽孢杆菌GS01菌株是从人参的根中分离出来的，能够分解植物细胞壁; 并通过用具有选自SEQ ID NO：3至SEQ ID NO：8的任何一个核苷酸序列的引物进行PCR（聚合酶链式反应）来分析基因cel44C。

公开(公告)号：KR1020080070127A

公开(公告)日：2008-07-30

申请号：KR1020070007760

申请日：2007-01-25

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  홍수영 ,  조계만

IPC: A23L1/238 ,  A23L1/202 ,  A23L1/28

CPC classification number: A23L27/50 ,  A23L29/065 ,  A23L31/00 ,  C12R1/07

Abstract: A method of rapidly producing fermented soybeans and soy sauce is provided to obtain fermented soybeans and soy sauce with a high content of surfactin exhibiting cytotoxicity against breast cancer cells and intestinal cancer. Bacillus pumilus HY1 strains(KACC 91280P) having excellent surfactin producing capacity are isolated from Korean traditional soy sauce. Surfactin and 5 types of isomers are identified from the Bacillus pumilus HY1 strains. Soy sauce is rapidly prepared by using the Bacillus pumilus HY1 strains as a starter. The soy sauce using Bacillus pumilus HY1 strains as a starter is fermented about 15 days faster than untreated control group. The surfactin content of soy sauce 45 days after fermentation exhibits 0.008mg/L in case the Bacillus pumilus HY1 strains are inoculated, as compared with 0.005mg/L in case the Bacillus pumilus HY1 strains are not inoculated.

Abstract translation: 提供快速生产发酵大豆和酱油的方法，以获得具有高表达活性的含量的发酵大豆和酱油，其对乳腺癌细胞和肠癌具有细胞毒性。 从韩国传统酱油中分离出具有优异表面活性素生产能力的短小芽孢杆菌HY1菌株（KACC 91280P）。 从短小芽孢杆菌HY1菌株鉴定枯草芽孢杆菌素和5种异构体。 通过使用短小芽孢杆菌HY1菌株作为起始物快速制备酱油。 使用短小芽孢杆菌HY1菌株作为起始物的酱油比未处理的对照组发酵约15天。 在接种短小芽孢杆菌HY1菌株的情况下，发酵45天后的酱油的表面活性素含量为0.008mg / L，与未接种短小芽孢杆菌HY1菌株相比，为0.005mg / L。