公开(公告)号：KR1020090006675A

公开(公告)日：2009-01-15

申请号：KR1020070070260

申请日：2007-07-12

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  이영한 ,  조계만

IPC: C12N1/20

Abstract: A Bacillus humi analogous strain, a method for preparing the compost by using the Bacillus humi analogous strain, and the organic compost prepared by the method are provided to improve the quality of organic compost and to reduce the fermentation time. A Bacillus humi analogous strain comprises the sequence represented by the sequence number 1. A method for preparing the compost comprises the step of using the excretions of domestic animals and the mushroom culture waste sawdust culture medium in the roller system using the Bacillus humi analogous strain. Preferably the excretions of domestic animals and the mushroom culture waste sawdust culture medium are mixed in a ratio of 8:2.

Abstract translation: 提供芽孢杆菌类似菌株，通过使用芽孢杆菌类似菌株制备堆肥的方法和通过该方法制备的有机堆肥，以提高有机堆肥的质量并减少发酵时间。 芽孢杆菌类似菌株包含由序列号1表示的序列。制备堆肥的方法包括在使用芽孢杆菌类似菌株的辊系统中使用家畜和蘑菇培养废木屑培养基的排泄物的步骤。 优选将家畜和蘑菇培养废砂屑培养基的排泄物以8:2的比例混合。

公开(公告)号：KR100851063B1

公开(公告)日：2008-08-12

申请号：KR1020070007760

申请日：2007-01-25

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  홍수영 ,  조계만

IPC: A23L1/238 ,  A23L1/202 ,  A23L1/28

Abstract: 본 발명은 한국 전통 간장으로부터 분리한 바실러스 퍼밀러스 HY1균주(
Bacillus pumilus HY1 )를 동정하고 상기 균주로부터 생성되는 설펙틴의 분리 및 정제 방법, 상기 균주로부터 분리된 설펙틴의 유방암과 대장암에 대한 세포독성 시험 및 바실러스 퍼밀러스 (
Bacillus

pumilus ) HY1를 이용하여 설펙틴이 다량 함유된 청국장 및 간장의 속성 제조방법을 제공하는 뛰어난 효과가 있다.
바실러스 퍼밀러스, 설펙틴, 청국장, 간장 속성제조법

公开(公告)号：KR100729938B1

公开(公告)日：2007-06-19

申请号：KR1020060078126

申请日：2006-08-18

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  홍수영

IPC: C12N9/24 ,  C12N9/00

Abstract: A novel multifunctional gene cel44C isolated from a Paenibacillus polymyxa GS01 strain is provided to decompose the cell wall of a plant and simultaneously decompose cellulose, xylan, lichenan and mannan, so that the gene is useful for paper industry and organic food production. The novel multifunctional gene cel44C isolated from a Paenibacillus polymyxa GS01 strain has the nucleotide sequence of SEQ ID NO:1, wherein the multifunctional enzyme encoded by the gene cel44C has the amino acid sequence of SEQ ID NO:2; the Paenibacillus polymyxa GS01 strain is isolated from the root of ginseng and is capable of decomposing plant cell wall; and the gene cel44C is analyzed by performing PCR(polymerase chain reaction) with a primer having any one nucleotide sequence selected from SEQ ID NO:3 to SEQ ID NO:8.

Abstract translation: 提供了一种从多粘类芽孢杆菌GS01菌株中分离出的新型多功能基因cel44C，用于分解植物的细胞壁并同时分解纤维素，木聚糖，地衣多糖和甘露聚糖，使得该基因可用于造纸工业和有机食品生产。 从多粘类芽孢杆菌GS01菌株中分离的新型多功能基因cel44C具有SEQ ID NO：1的核苷酸序列，其中由基因cel44C编码的多功能酶具有SEQ ID NO：2的氨基酸序列; 多粘类芽孢杆菌GS01菌株是从人参的根中分离出来的，能够分解植物细胞壁; 并通过用具有选自SEQ ID NO：3至SEQ ID NO：8的任何一个核苷酸序列的引物进行PCR（聚合酶链式反应）来分析基因cel44C。

公开(公告)号：KR1020080102873A

公开(公告)日：2008-11-26

申请号：KR1020070049884

申请日：2007-05-22

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  김민근

IPC: C12P7/54

CPC classification number: A01G18/00 ,  C07D209/12 ,  C12P17/10

Abstract: A method of separating indole acetic acid from Pseudomonas strain is provided to promote rearing of mushroom, to reduce considerably a cost for producing oil and a labor cost and to be used in order to develop mushroom culture medium for the low cost and to culture other edible mushrooms besides the pine mushroom, gardening and vegetables. An indole acetic acid is separated from Pseudomonas sp. 7014 KACC 91277P strain culture fluid or an ethylacetate layer extract of the culture fluid. A method of stimulating a growth of the edible mushrooms comprises a step of mixing the indole acetic acid and an amino acid at a rate of 1:1(v/v). The ethylacetate layer extract has an activity of stimulating a growth of the Pseudomonas sp. 7014 KACC 91277P strain.

Abstract translation: 提供了从假单胞菌属菌株中分离吲哚乙酸的方法，以促进蘑菇的饲养，大大降低生产油的成本和劳动成本，并用于低成本开发蘑菇培养基并培养其他可食用 蘑菇除松蘑菇，园艺和蔬菜外。 吲哚乙酸与假单胞菌属（Pseudomonas sp。）分离。 7014 KACC 91277P菌株培养液或培养液的乙酸乙酯层提取物。 刺激可食用蘑菇生长的方法包括以1：1（v / v）的速率混合吲哚乙酸和氨基酸的步骤。 乙酸乙酯层提取物具有刺激假单胞菌属（Pseudomonas sp。）生长的活性。 7014 KACC 91277P菌株。

公开(公告)号：KR1020080070127A

公开(公告)日：2008-07-30

申请号：KR1020070007760

申请日：2007-01-25

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  홍수영 ,  조계만

IPC: A23L1/238 ,  A23L1/202 ,  A23L1/28

CPC classification number: A23L27/50 ,  A23L29/065 ,  A23L31/00 ,  C12R1/07

Abstract: A method of rapidly producing fermented soybeans and soy sauce is provided to obtain fermented soybeans and soy sauce with a high content of surfactin exhibiting cytotoxicity against breast cancer cells and intestinal cancer. Bacillus pumilus HY1 strains(KACC 91280P) having excellent surfactin producing capacity are isolated from Korean traditional soy sauce. Surfactin and 5 types of isomers are identified from the Bacillus pumilus HY1 strains. Soy sauce is rapidly prepared by using the Bacillus pumilus HY1 strains as a starter. The soy sauce using Bacillus pumilus HY1 strains as a starter is fermented about 15 days faster than untreated control group. The surfactin content of soy sauce 45 days after fermentation exhibits 0.008mg/L in case the Bacillus pumilus HY1 strains are inoculated, as compared with 0.005mg/L in case the Bacillus pumilus HY1 strains are not inoculated.

Abstract translation: 提供快速生产发酵大豆和酱油的方法，以获得具有高表达活性的含量的发酵大豆和酱油，其对乳腺癌细胞和肠癌具有细胞毒性。 从韩国传统酱油中分离出具有优异表面活性素生产能力的短小芽孢杆菌HY1菌株（KACC 91280P）。 从短小芽孢杆菌HY1菌株鉴定枯草芽孢杆菌素和5种异构体。 通过使用短小芽孢杆菌HY1菌株作为起始物快速制备酱油。 使用短小芽孢杆菌HY1菌株作为起始物的酱油比未处理的对照组发酵约15天。 在接种短小芽孢杆菌HY1菌株的情况下，发酵45天后的酱油的表面活性素含量为0.008mg / L，与未接种短小芽孢杆菌HY1菌株相比，为0.005mg / L。

公开(公告)号：KR100817321B1

公开(公告)日：2008-03-27

申请号：KR1020060101510

申请日：2006-10-18

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만

IPC: C12N15/55 ,  C12N15/10

Abstract: A sequence of an esterase coding gene(est5S) is provided to be isolated from ruminant stomach bacteria, which is not be cultured by a conventional technique. An esterase is provided to be a novel esterase not belonging to a conventional fat hydrolysis group and show significant effect on metal ions and organic solvent, thereby being useful for food industry, environment industry, agricultural industry and medicinal industry. A novel esterase gene hydrolyzing fat includes a sequence described as SEQ ID : NO. 1 derived from ruminant stomach bacteria identified from cow rumen metagenome. An esterase hydrolyzing the fat includes an amino acid sequence described as SEQ ID : NO. 2 derived from the ruminant stomach bacteria identified from cow rumen metagenome.

Abstract translation: 提供酯酶编码基因（est5S）的序列，从反刍动物胃细菌中分离出来，不用常规技术培养。 提供酯酶作为不属于常规脂肪水解基团的新型酯酶，对金属离子和有机溶剂具有显着影响，从而可用于食品工业，环境工业，农业和医药行业。 水解脂肪的新型酯酶基因包含SEQ ID NO： 1来源于反刍动物胃细菌，从牛瘤胃巨噬细胞中鉴定。 水解脂肪的酯酶包括如SEQ ID NO：1所示的氨基酸序列。 2来源于从牛瘤胃巨噬细胞中鉴定的反刍动物胃细菌。

公开(公告)号：KR1020080066295A

公开(公告)日：2008-07-16

申请号：KR1020070003529

申请日：2007-01-11

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  김민근 ,  조계만

IPC: C12N1/20 ,  C12N1/00

Abstract: A Pseudomonas sp. 7014 strain is provided to shorten a total cultivation period of king oyster mushroom by promoting growth of mushroom mycelium and reduce manufacturing cost and labor cost significantly, thereby being very useful for cultivating other mushrooms as well as the king oyster mushroom, for gardening and for cultivating vegetables. A Pseudomonas sp. 7014 strain having the growth promoting activity during cultivation of bottle mushroom is deposited as a deposition no. KACC 91277P. A method for rapid cultivation of the bottle mushroom comprises the steps of: (a) preparing a culture medium by mixing the Oregon pine, Salix koreensis, wheat bran and rice bran in a volumetric ratio of 5-7:1-3:0.5-1.5:0.5-1.5, adding tap water thereto to adjust a moisture content to be 60-70%, filling up a plastic bottle with the mixture, applying mechanical perforation thereto and then sterilizing it; and (b) after inoculating the Pseudomonas sp. strain into the culture medium and allowing it to be fixated, inoculating mushroom sawdust spawn thereinto and culturing.

Abstract translation: 假单胞菌属 提供7014菌株，通过促进蘑菇菌丝体的生长，缩短蚝菇的总栽培期，显着降低制造成本和劳动力成本，因此对于栽培其他蘑菇以及国王牡蛎蘑菇，园艺栽培非常有用 蔬菜。 假单胞菌属 7014菌株在培养瓶蘑期间具有生长促进活性。 KACC 91277P。 一种用于快速栽培瓶子的方法包括以下步骤：（a）通过以5-7:1-3:0.5:1的体积比混合俄勒冈松，柳桉，麦麸和米糠来制备培养基， 1.5：0.5-1.5，加入自来水以调节水分含量为60-70％，用混合物填充塑料瓶，对其进行机械穿孔，然后对其进行消毒; 和（b）接种假单胞菌属 将其置入培养基中并使其固定，接种蘑菇锯屑并进行培养。

公开(公告)号：KR1020110123540A

公开(公告)日：2011-11-15

申请号：KR1020100043068

申请日：2010-05-07

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  윤명근 ,  김종민 ,  조지중

IPC: C12N11/14 ,  C12N1/20 ,  C12R1/01

Abstract: PURPOSE: A novel microorganism carrier using bottom ash is provided to enhance stable survival rate of microorganism. CONSTITUTION: A microorganism fixation carrier is manufactured by adding a coating material to a microorganism carrier. The coating material is polyvinyl alcohol(PVA) or CMC. A method for manufacturing the microorganism fixing carrier comprises: a step of alternatively performing spore formation and coating method; and a step of preparing a microorganism carrier using bottom ash. The spore formation is performed using TSB medium containing 50mg/L of MnSO_4·H_2O and alternatively using 5% PVA solution.

Abstract translation: 目的：提供使用底灰的新型微生物载体，以提高微生物的稳定存活率。 构成：通过向微生物载体添加涂层材料来制造微生物固定载体。 涂料是聚乙烯醇（PVA）或CMC。 微生物固定载体的制造方法包括：交替进行孢子形成和涂布方法的步骤; 以及使用底灰制备微生物载体的步骤。 使用含有50mg / L MnSO 4·H 2 O的TSB培养基，或者使用5％PVA溶液进行孢子形成。

公开(公告)号：KR1020100027742A

公开(公告)日：2010-03-11

申请号：KR1020080086772

申请日：2008-09-03

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  조지중

IPC: A23B7/10 ,  A23L19/20 ,  C11D3/386 ,  C12N9/02

CPC classification number: A23B7/10 ,  A23L19/20 ,  C11D3/386 ,  C12N9/001

Abstract: PURPOSE: Organophosphorus hydrolases effectively used for removing organophosphorus insecticide are provided to effectively decompose the organophosphorus insecticide and to confirm an optimized fermentation condition of young radish watery Kimchi with degrading lactic acid bacteria. CONSTITUTION: A method for fermenting of young radish watery Kimchi includes the following steps: salting young radish in salt water for 3 ~ 5 hours; washing the salted young radish; adjusting the slat concentration to 2 ~ 4 %; mixing wheat flour with water; manufacturing wheat flour liquid; mixing the wheat flour liquid with the young radish; making a seasoning for the kimchi; adding the seasoning in the young radish; and fermenting the young radish for 10 ~ 15 days.

Abstract translation: 目的：有效利用有机磷杀虫剂去除有机磷水解酶，有效分解有机磷杀虫剂，确定年轻萝卜水泡菜的优化发酵条件，降解乳酸菌。 构成：萝卜水煮泡菜的发酵方法包括以下步骤：将咸萝卜在咸水中盐化3〜5小时; 洗盐渍的年轻萝卜; 调整板条浓度至2〜4％; 将小麦粉与水混合; 制造小麦粉液体; 将小麦面粉液与青萝卜混合; 为泡菜做调味料; 在萝卜中加入调味料; 并将萝卜萝卜发酵10〜15天。

公开(公告)号：KR1020100022355A

公开(公告)日：2010-03-02

申请号：KR1020080080991

申请日：2008-08-19

Applicant: 경상대학교산학협력단

Inventor： 윤한대 ,  조계만 ,  김종민

IPC: C12N15/10 ,  C12N15/31 ,  C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6827

Abstract: PURPOSE: A method for detecting lactobacillus in Kimchi fermentation through multiplex PCR is provided to accurately and quickly detect grouping pattern of lactobacillus and recue time and cost. CONSTITUTION: A primer set for detecting lactobacillus in Kimchi fermentation using multiplex PCR comprises a primer pair of sequence number 1 and 2, 3 and 4, 5 and 6, 7 and 8, 9 and 10, 11 and 12, 13 and 14, 15 and 16, 17 and 18, or 19 and 20. A method for detecting lactobacillus in Kimchi fermentation comprises: a step of making a primer set which is specific to lactobacillus relating to Kimchi fermentation; a step of collecting a sample from Kimchi fermented 4°C~25°C in a specific time interval; a step of measuring change of strain number; a step of irritation; a step of confirming 10 kinds of lactobacillus by performing m-PCR; a step of confirming with a 16S rDNA(ribosomal DNA); and a step of detecting final m-PCR.

Abstract translation: 目的：提供通过多重PCR检测泡菜发酵乳杆菌的方法，准确，快速地检测乳酸菌的分组模式，并记录时间和成本。 构成：使用多重PCR在泡菜发酵中检测乳杆菌的引物组包括序列号1和2,3和4,5和6,7和8,9和10,11和12,13和14,15的引物对 在泡菜发酵中检测乳杆菌的方法包括：制备与泡菜发酵相关的乳杆菌特异性的引物组的步骤; 在特定的时间间隔内从泡菜收集样品4℃〜25℃的步骤; 测量应变数变化的一个步骤; 刺激的一步; 通过m-PCR确认10种乳酸杆菌的步骤; 用16S rDNA（核糖体DNA）确认的步骤; 以及检测最终m-PCR的步骤。