公开(公告)号：KR1020070117373A

公开(公告)日：2007-12-12

申请号：KR1020060051571

申请日：2006-06-08

Applicant: 경희대학교 산학협력단

Inventor： 정주호 ,  홍선표 ,  임성빈 ,  강민기 ,  홍미숙 ,  김학재 ,  박해정

IPC: A61K36/752 ,  A61P35/00 ,  A61P29/00

CPC classification number: A61K36/752 ,  A61K2236/39 ,  Y10S514/886

Abstract: A therapeutic agent HJ001 isolated from Citrus reticulata Blanco is provided to inhibit growth of cancer cells and reduce expressions of IL-3(interleukin-3), IL-6, IL-12p40p70, IL-12p70 and SCF(stem cell factor) and increase expressions of IL-10 and TNF(tumor necrosis factor), so that it is useful for treatment of cancer and inflammatory disease. A method for preparing the extract of Citrus reticulata Blanco HJ001 comprises the steps of: dipping Citrus reticulata Blanco powder in water and filtering the solution; passing the filtered solution through the column packed with fillers HP-20 and collecting passed solution; mixing the collected solution with water-saturated ethylacetate to transfer it into an ethylacetate layer; inducing layer separation of the mixture into the ethylacetate layer and water layer, and separating the water layer; mixing the water layer with water-saturated butanol and separating a butanol layer; absorbing the butanol layer to TLC(thin layer chromatography) silica; subjecting the TLC silica by using chloroform, methanol and water as development solvent and separating the developed portion; dissolving the developed portion in butanol, and filtering and concentrating the solution; freeze-drying the filtered solution to obtain materials Fr1 and Fr2; and separating the Fr2 from the Fr1 and Fr2.

Abstract translation: 提供从柑橘网布兰科分离的治疗剂HJ001，以抑制癌细胞的生长并降低IL-3（白细胞介素-3），IL-6，IL-12p40p70，IL-12p70和SCF（干细胞因子）的表达并增加 IL-10和TNF（肿瘤坏死因子）的表达，因此可用于治疗癌症和炎性疾病。 柑橘柑橘提取物Blanco HJ001的制备方法，包括：将柑橘网Blanco粉浸入水中过滤溶液; 将过滤的溶液通过填充有填料HP-20的柱并收集通过的溶液; 将收集的溶液与水饱和的乙酸乙酯混合以将其转移到乙酸乙酯层中; 诱导层将混合物分离成乙酸乙酯层和水层，并分离水层; 将水层与水饱和的丁醇混合并分离丁醇层; 将丁醇层吸收至TLC（薄层色谱法）二氧化硅; 用氯仿，甲醇和水作为显影溶剂对TLC二氧化硅进行处理，分离显影部分; 将显影部分溶解在丁醇中，过滤并浓缩溶液; 冷冻干燥过滤溶液以获得材料Fr1和Fr2; 并将Fr2与Fr1和Fr2分离开。