公开(公告)号：KR1020070117373A

公开(公告)日：2007-12-12

申请号：KR1020060051571

申请日：2006-06-08

Applicant: 경희대학교 산학협력단

Inventor： 정주호 ,  홍선표 ,  임성빈 ,  강민기 ,  홍미숙 ,  김학재 ,  박해정

IPC: A61K36/752 ,  A61P35/00 ,  A61P29/00

CPC classification number: A61K36/752 ,  A61K2236/39 ,  Y10S514/886

Abstract: A therapeutic agent HJ001 isolated from Citrus reticulata Blanco is provided to inhibit growth of cancer cells and reduce expressions of IL-3(interleukin-3), IL-6, IL-12p40p70, IL-12p70 and SCF(stem cell factor) and increase expressions of IL-10 and TNF(tumor necrosis factor), so that it is useful for treatment of cancer and inflammatory disease. A method for preparing the extract of Citrus reticulata Blanco HJ001 comprises the steps of: dipping Citrus reticulata Blanco powder in water and filtering the solution; passing the filtered solution through the column packed with fillers HP-20 and collecting passed solution; mixing the collected solution with water-saturated ethylacetate to transfer it into an ethylacetate layer; inducing layer separation of the mixture into the ethylacetate layer and water layer, and separating the water layer; mixing the water layer with water-saturated butanol and separating a butanol layer; absorbing the butanol layer to TLC(thin layer chromatography) silica; subjecting the TLC silica by using chloroform, methanol and water as development solvent and separating the developed portion; dissolving the developed portion in butanol, and filtering and concentrating the solution; freeze-drying the filtered solution to obtain materials Fr1 and Fr2; and separating the Fr2 from the Fr1 and Fr2.

Abstract translation: 提供从柑橘网布兰科分离的治疗剂HJ001，以抑制癌细胞的生长并降低IL-3（白细胞介素-3），IL-6，IL-12p40p70，IL-12p70和SCF（干细胞因子）的表达并增加 IL-10和TNF（肿瘤坏死因子）的表达，因此可用于治疗癌症和炎性疾病。 柑橘柑橘提取物Blanco HJ001的制备方法，包括：将柑橘网Blanco粉浸入水中过滤溶液; 将过滤的溶液通过填充有填料HP-20的柱并收集通过的溶液; 将收集的溶液与水饱和的乙酸乙酯混合以将其转移到乙酸乙酯层中; 诱导层将混合物分离成乙酸乙酯层和水层，并分离水层; 将水层与水饱和的丁醇混合并分离丁醇层; 将丁醇层吸收至TLC（薄层色谱法）二氧化硅; 用氯仿，甲醇和水作为显影溶剂对TLC二氧化硅进行处理，分离显影部分; 将显影部分溶解在丁醇中，过滤并浓缩溶液; 冷冻干燥过滤溶液以获得材料Fr1和Fr2; 并将Fr2与Fr1和Fr2分离开。

公开(公告)号：KR1020110020073A

公开(公告)日：2011-03-02

申请号：KR1020090077777

申请日：2009-08-21

Applicant: 경희대학교 산학협력단

Inventor： 정주호 ,  김재영 ,  김경남 ,  홍미숙 ,  김학재

IPC: A61K31/192 ,  A61P25/16

Abstract: PURPOSE: A pharmaceutical composition containing tranexamic acid for preventing or treating Parkinson's disease is provided to prevent apoptosis of nerve cells in neuroblastoma(SH-SY5Y) and to suppress caspase 3 activation. CONSTITUTION: A pharmaceutical composition for preventing or treating Parkinson's disease or similar disease contains tranexamic acid of chemical formula 1 or pharmaceutically acceptable salt thereof as an active ingredient. The compound of chemical formula 1 forms an additive salt by reacting with pharmaceutically acceptable inorganic acid, organic acid, or base. The base is sodium, potassium, magnesium, or calcium. The inorganic acid is hydrochloric acid, or sulfuric acid. The organic acid is acetic acid or maleic acid. The composition is manufactured in the form of tablet, powder, elixir, suspension, emulsion, solution, syrup, aerosol, soft or hard gelatin capsule, or sterilized injection solution.

Abstract translation: 目的：提供含有用于预防或治疗帕金森病的凝血酸的药物组合物，以防止神经母细胞瘤（SH-SY5Y）中神经细胞的凋亡并抑制半胱天冬酶3的活化。 构成：用于预防或治疗帕金森病或类似疾病的药物组合物含有化学式1的氨甲环酸或其药学上可接受的盐作为活性成分。 化学式1的化合物通过与药学上可接受的无机酸，有机酸或碱反应形成添加剂盐。 碱是钠，钾，镁或钙。 无机酸是盐酸或硫酸。 有机酸是乙酸或马来酸。 组合物以片剂，粉末，酏剂，悬浮液，乳剂，溶液，糖浆，气雾剂，软或硬明胶胶囊或灭菌注射液的形式制备。

公开(公告)号：KR1020100104930A

公开(公告)日：2010-09-29

申请号：KR1020090023667

申请日：2009-03-19

Applicant: 경희대학교 산학협력단

Inventor： 홍미숙 ,  박히준 ,  정주호 ,  이혜정

IPC: C12Q1/68 ,  C12N15/63

CPC classification number: C12Q1/6837 ,  C12N15/63 ,  G01N33/5008 ,  G01N33/6896 ,  G01N2500/00

Abstract: PURPOSE: A cDNA microarray chip for diagnosing Parkinson's disease is provided to monitor gene expression pattern from a patient with Parkinson's diseases and to easily select a target gene. CONSTITUTION: A microarray chip for diagnosing Parkinson's diseases contains a gene of which expression is specifically increased or decreased in MPTP(1-methyl-4phenyl-1,2,3,6-tetrahydropyridine). The gene is calcium signaling pathway-relating gene and receptor thereof, cyclin AMP pathway-relating gene, or transcription factor. A method for screening a therapeutic agent for Parkinson's disease is performed using microarray chip. The therapeutic agent for Parkinson's disease is a material which controls expression level by binding to antisense oligonucleotide, peptide, small molecular compound, antibody or target gene promoter.

Abstract translation: 目的：提供用于诊断帕金森病的cDNA微阵列芯片，用于监测患有帕金森病的患者的基因表达模式，并容易地选择靶基因。 构成：用于诊断帕金森病的微阵列芯片含有在MPTP（1-甲基-4-苯基-1,2,3,6-四氢吡啶）中表达特异性增加或降低的基因。 该基因是钙信号通路相关基因及其受体，细胞周期蛋白AMP途径相关基因或转录因子。 使用微阵列芯片进行用于筛选帕金森病治疗剂的方法。 帕金森病的治疗剂是通过与反义寡核苷酸，肽，小分子化合物，抗体或靶基因启动子结合来控制表达水平的物质。