公开(公告)号：KR100797152B1

公开(公告)日：2008-01-23

申请号：KR1020060116688

申请日：2006-11-24

Applicant: 고려대학교 산학협력단

Inventor： 김승욱 ,  강성우 ,  이종호 ,  김영환 ,  장효일 ,  윤철원 ,  백현동 ,  강창원

IPC: C12N1/16

Abstract: A method for improving production yield of Saccharomyces cerevisiae JUL3 having beta-glucan is provided to mass produce Saccharomyces cerevisiae JUL3 inexpensively by using an inexpensive medium and optimizing process parameters, and increase the content of beta-glucan having high physiological activity in Saccharomyces cerevisiae JUL3. A method for improving production yield of Saccharomyces cerevisiae JUL3(KFCC 11359P) having abundant beta-glucan comprises the steps of: determining the optimum medium composition, wherein the optimum medium compositions are 5-7% molasses, 15-19% corn steep liquor, 0.3-0.7% KH2PO4, 0.05-0.15% MgSO4 and trace amount of minerals through response surface methodology as a statistical analysis method; comparing the cell production amount of Saccharomyces cerevisiae JUL3 in the optimum medium composition; determining the culture conditions, wherein the culture conditions are 200-400 rpm and 1-3 vvm in 2.5 liter fermenter, which are required for scale-up by using the optimum medium composition; and optimizing the substrate feed rate(10-20 ml/h) and concentration(30-75%) required for optimization of fed-batch culture under the optimum medium composition and culture conditions.

Abstract translation: 提供了具有β-葡聚糖的酿酒酵母JUL3的产量提高的方法，通过使用便宜的培养基和优化工艺参数廉价地大量生产酿酒酵母JUL3，并且增加了在酿酒酵母JUL3中具有高生理活性的β-葡聚糖的含量。 提供具有丰富β-葡聚糖的酿酒酵母JUL3（KFCC 11359P）的生产产量的方法包括以下步骤：确定最佳培养基组成，其中最佳培养基组合物为5-7％糖蜜，15-19％玉米浆， 作为统计分析方法，通过响应面方法，0.3-0.7％KH 2 PO 4，0.05-0.15％MgSO 4和微量矿物质; 将酿酒酵母JUL3的细胞产生量与最佳培养基组成进行比较; 确定培养条件，其中培养条件为200-400rpm，2.5升发酵罐中1-3vvm，其通过使用最佳培养基组合物进行放大所需; 并优化在最佳培养基组成和培养条件下优化补料分批培养所需的底物进料速率（10-20ml / h）和浓度（30-75％）。

公开(公告)号：KR100700910B1

公开(公告)日：2007-03-28

申请号：KR1020050111224

申请日：2005-11-21

Applicant: 고려대학교 산학협력단

Inventor： 장효일 ,  하창훈 ,  강창원 ,  김승욱 ,  백현동 ,  윤철원

IPC: C12N1/16

Abstract: A novel Saccharomyces cerevisiae mutant is provided to be able to produce alkali-soluble beta-glucan having immuno-activity with high yield, thereby being applied to various products having immuno-activity such as functional foods and cosmetics. The Saccharomyces cerevisiae mutant producing alkali-soluble beta-glucan is deposited in a Korean Culture Center of Microorganisms(KCCM) as a deposition number of KFCC-11359P. The method for preparing the Saccharomyces cerevisiae mutant comprises the steps of: (a) applying UV to yeast; (b) firstly screening the mutant yeast survived after the UV radiation; (c) after adding lyticase to the firstly screened yeast to cleave cell wall, performing the osmotical lysis using distilled water; and (d) secondly screening the lyticase resistant yeast survived after the lysis. The method for producing the alkali-soluble beta-glucan comprises the steps of: (a) culturing the Saccharomyces cerevisiae mutant KFCC-11359P; (b) treating the cultured mutant with NaOH to perform an alkali extraction; and (c) treating the product obtained from the step(b) with cetavlon and borate to selectively precipitate mannoprotein.

Abstract translation: 提供了一种新型酿酒酵母突变体以能够以高产率生产具有免疫活性的碱溶性β-葡聚糖，从而应用于具有功能性食品和化妆品等免疫活性的各种产品。 产生碱溶性β-葡聚糖的酿酒酵母突变体作为KFCC-11359P的保藏号沉积在韩国文化微生物中心（KCCM）中。 制备酿酒酵母突变体的方法包括以下步骤：（a）将紫外线施加于酵母; （b）首先筛选在紫外线辐射后存活的突变酵母; （c）在首先筛选的酵母中加入溶解酶以切割细胞壁后，使用蒸馏水进行渗透裂解; 和（d）第二次筛选溶解酶的酵母在裂解后存活。 制备碱溶性β-葡聚糖的方法包括以下步骤：（a）培养酿酒酵母突变体KFCC-11359P; （b）用NaOH处理培养的突变体进行碱提取; 和（c）用鲸蜡酮和硼酸盐处理由步骤（b）获得的产物以选择性沉淀甘露糖蛋白。