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公开(公告)号：KR101135054B1
公开(公告)日：2012-04-13
申请号：KR1020080128786
申请日：2008-12-17
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 글루타티온 S 트랜스퍼라제 태깅(GST tagging) 단백질 분리용 나노입자와 제조 방법 및 이를 이용한 단백질 분리 정제 방법에 관한 것으로서, 보다 상세하게는 표면에 글루타티온기가 결합된 자성 나노입자를 이용하여 글루타티온 S 트랜스퍼라제 태깅(GST tagging) 단백질을 분리 정제하는 방법에 관한 것이다. 본 발명에 따르면 특정 단백질을 매우 선택적으로 간단하고 신속하게 분리할 수 있어, 암과 같은 질병 치료 연구에 유용하게 사용될 수 있다.
자성 나노입자, 펩티드, 시스테인-
公开(公告)号：KR1020100070171A
公开(公告)日：2010-06-25
申请号：KR1020080128786
申请日：2008-12-17
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A nanoparticle for isolating proteins is provided to isolate and purify GST-tagged protein using a magnetic nanoparticle on which glutathione is conjugated. CONSTITUTION: A nanoparticle for isolating GST-tagged protein has a magnetic nanoparticle surface on which glutathione group is conjugated. The magnetic nanoparticle is conjugated with a silica layer. The glutathione group is conjugated with 2-pyridyl disulfide which is bound to the silica layer. A method for manufacturing the nanoparticle for isolating the GST tagged protein comprises: a step of coating the surface of magnetic nanoparticle with the silica layer; a step of binding 2-pyridyl disulfide group to the silica layer; and a step of binding glutathione group to 2-pyridyldisulfide group.
Abstract translation: 目的：提供用于分离蛋白质的纳米颗粒，以使用其上结合有谷胱甘肽的磁性纳米颗粒分离和纯化GST标记的蛋白质。 构成：用于分离GST标记的蛋白质的纳米颗粒具有结合有谷胱甘肽基团的磁性纳米颗粒表面。 磁性纳米颗粒与二氧化硅层共轭。 谷胱甘肽基与与二氧化硅层结合的2-吡啶基二硫键缀合。 用于制造用于分离GST标记蛋白的纳米颗粒的方法包括：用二氧化硅层涂覆磁性纳米颗粒的表面的步骤; 将二吡啶基二硫键与二氧化硅层结合的步骤; 和将谷胱甘肽基团结合到2-吡啶基二硫基的步骤。
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公开(公告)号：KR1020090056868A
公开(公告)日：2009-06-03
申请号：KR1020080119056
申请日：2008-11-27
Applicant: 고려대학교 산학협력단
CPC classification number: B82Y5/00 , G01N33/54333 , G01N33/587 , Y10T428/2982
Abstract: A nanoparticle for peptide separation and a preparation method thereof are provided to rapidly and selectively separate desired peptide through a simple process owing to magnetic nanoparticles of which the surface is coupled with a first functional group which selectively reacts to a certain peptide. A nanoparticle for peptide separation has the magnetic surface which is coupled with a first functional group having thiol selectivity. The nanoparticle for peptide separation selectively couples with the peptide including cysteine. The first functional group includes 2-pyridyl disulfide residual group. The magnetic nanoparticle is coated with a silica layer. The first functional group is bonded with the silica layer. The surface of the nanoparticle additionally contains a second functional group which prevents the coherence of the nanoparticles.
Abstract translation: 提供了用于肽分离的纳米颗粒及其制备方法，通过简单的方法快速且有选择地分离所需的肽，因为磁性纳米颗粒与表面与选择性地与特定肽反应的第一官能团偶联。 用于肽分离的纳米颗粒具有与具有硫醇选择性的第一官能团偶联的磁性表面。 用于肽分离的纳米颗粒选择性地与包括半胱氨酸的肽结合。 第一官能团包括2-吡啶基二硫化物残基。 磁性纳米颗粒涂覆有二氧化硅层。 第一官能团与二氧化硅层结合。 纳米颗粒的表面还含有防止纳米颗粒的相干性的第二官能团。
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公开(公告)号：KR101077966B1
公开(公告)日：2011-10-31
申请号：KR1020080119056
申请日：2008-11-27
Applicant: 고려대학교 산학협력단
CPC classification number: B82Y5/00 , G01N33/54333 , G01N33/587 , Y10T428/2982
Abstract: 본발명은펩티드분리용나노입자와제조방법및 이를이용한펩티드분리방법에관한것으로서, 보다상세하게는자성나노입자의표면에티올(thiol) 선택성을갖는제1기능기가결합되어, 시스테인을포함하는펩티드와선택적으로결합하는펩티드분리용나노입자를이용하여특정펩티드를분리하는방법에관한것이다. 본발명에따르면특정펩티드를매우선택적으로간단하고신속하게분리할수 있어, 암과같은질병치료연구에유용하게사용될수 있다.
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