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Patent Agency Ranking
公开(公告)号：KR100883397B1
公开(公告)日：2009-02-11
申请号：KR1020070034231
申请日：2007-04-06
Applicant: 고려대학교 산학협력단
CPC classification number: Y02P20/544
Abstract: 본 발명은 유지와 알코올을 기질로 하고 리파아제를 이용하여 바이오디젤을 생산하는 효소공정에 초임계 유체를 동시에 적용시키는 단계를 포함하는 것을 특징으로 하는 바이오디젤 생산 방법에 관한 것이다.
본 발명에 따르면, 성질이 다른 두 종류의 리파아제인 1.3-위치특이성 리파아제와 무위치 특이성 리파아제를 사용하는 효소공정에 초임계 이산화탄소를 동시에 적용시키는 바이오디젤 생산방법으로 바이오디젤의 생산시간을 최소화 시키며 생산효율을 극대화 시킬 수 있다.
유지, 알코올, 리파아제, 바이오디젤, 1.3-위치특이성 리파아제, 무위치 특이성 리파아제, 초임계 이산화탄소-
公开(公告)号：KR100817775B1
公开(公告)日：2008-03-31
申请号：KR1020070023007
申请日：2007-03-08
Applicant: 고려대학교 산학협력단
Abstract: A method for pretreating lipase before its immobilization is provided to improve activity of lipase when it is immobilized on the carrier without use of complicated procedures such as control of carrier pore size or addition of other additives during immobilization, so that the expensive lipase is reused repeatedly without loss of activity. A pretreatment of lipase is carried out by reacting lipase with a fatty acid as a substrate of lipase or oil before its immobilization to combine the fatty acid to the active site of lipase, so that the active site of lipase is protected, wherein the fatty acid is a fatty acid having the carbon number of 16-18 such as linoleic acid, oleic acid, stearic acid or palmitic acid, and the oil is vegetable oil such as olive oil, soybean oil or palm oil.
Abstract translation: 提供在固定之前预处理脂肪酶的方法，以便在固定化载体时不使用诸如控制载体孔径或在固定期间添加其它添加剂的复杂程序来改善脂肪酶的活性，从而使昂贵的脂肪酶重复使用 而不会失去活动。 脂肪酶的预处理是通过使脂肪酶与作为脂肪酶或油的底物的脂肪酸反应进行的，然后将其脂肪酸与脂肪酶的活性位点结合，从而保护脂肪酶的活性位点，其中脂肪酸 是碳原子数16-18的脂肪酸，如亚油酸，油酸，硬脂酸或棕榈酸，油是植物油如橄榄油，大豆油或棕榈油。
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公开(公告)号：KR100746666B1
公开(公告)日：2007-08-06
申请号：KR1020050061337
申请日：2005-07-07
Applicant: 고려대학교 산학협력단
IPC: C12P1/02
Abstract: 본 발명은 페니실리움 시트리눔(
Penicillium
citrinum ) KCCM 11663 균주로부터 프락토실 트랜스퍼라제의 분리, 정제방법에 관한 것으로서, 상세하게는 수크로스(sucrose)가 포함된 배지조성에서 배양한 페니실리움 시트리눔 KCCM 11663이 생산하는 여러 효소 중 설탕을 네오프락토 올리고당으로 만드는 새로운 프락토실 트랜스퍼라제의 분리정제방법에 관한 것이다. 일반적으로는 프락토 올리고당과 네오프락토 올리고당이 함께 생산되었지만 본 발명의 정제된 프락토실 트랜스퍼라제를 이용하여 수크로스에 반응시킨 결과 프락토 올리고당은 생산되지 않고 네오프락토 올리고당만을 선택적으로 생산할 수 있어 여러 식품, 의약 산업의 첨가제로 사용이 용이하게 된다.
페니실리움 시트리눔 KCCM 11663, 프락토실 트랜스퍼라제, 수크로스, 네오플락토 올리고당-
公开(公告)号：KR1020070006210A
公开(公告)日：2007-01-11
申请号：KR1020050061337
申请日：2005-07-07
Applicant: 고려대학교 산학협력단
IPC: C12P1/02
Abstract: A method for purifying fructosyl transferase from Penicillium citrinum KCCM 11663 is provided to obtain the fructosyl transferase capable of selectively producing neo-fructo oligosaccharide without producing fructo-oligosaccharide. The method comprises the steps of: (a) preculturing Penicillium citrinum KCCM 11663 in a culture medium containing sucrose at a temperature of 15-35 deg.C with the speed of 100-30 rpm for 60-90 hours; (b) performing a shake-culturing of the preculture obtained from the step(a) in the same culture medium as the step(a) at a temperature of 15-35 deg.C with the speed of 400-700 rpm for 80-120 hours to mass-produce fructosyl transferase; (c) centrifuging the fermented solution of the step(b) to remove fungus bodies therefrom and the precipitating protein from the remaining solution by using ammonium sulfate to concentrate the protein; (d) after performing a first chromatography on the concentrated protein through a diethylaminoethyl-sepharose(DEAE-sepharose) column, eluting the protein using a salt having the concentration of 0.05-0.2M; (e) after performing a second chromatography on the eluted protein through a carboxymethyl-sepharose(CM-sepharose) column, eluting the protein using a salt having the concentration of 0.05-0.2M; and (f) performing a third chromatography on the eluted protein from the step(e) through a sephadex G75 column to purify the fructosyl transferase. In the method, the fructosyl transferase selectively produces neo-fructo oligosaccharide such as neokestose and neo-nystose from the sucrose.
Abstract translation: 提供了一种从青霉菌KCCM 11663中纯化果糖基转移酶的方法，以获得能够选择性地生产新的果寡糖而不产生低聚果糖的果糖基转移酶。 该方法包括以下步骤：（a）在含有蔗糖的培养基中，在15-35℃的温度下以100-30rpm的速度预培养柑桔青霉KCCM 11663 60-90小时; （b）在与步骤（a）相同的培养基中，在15-35℃的温度下以400-700rpm的速度进行步骤（a）获得的预培养物的摇动培养， 大量生产果糖基转移酶120小时; （c）通过使用硫酸铵将蛋白质的浓缩物离心分离步骤（b）的发酵溶液以从其中除去真菌，并从剩余的溶液中除去沉淀蛋白质; （d）通过二乙基氨基乙基琼脂糖凝胶（DEAE-sepharose）柱对浓缩的蛋白质进行第一次色谱之后，使用浓度为0.05-0.2M的盐洗脱该蛋白质; （e）通过羧甲基琼脂糖凝胶（CM-sepharose）柱对洗脱的蛋白质进行第二次色谱之后，使用浓度为0.05-0.2M的盐洗脱蛋白质; 和（f）通过sephadex G75柱对来自步骤（e）的洗脱的蛋白质进行第三层析以纯化果糖基转移酶。 在该方法中，果糖基转移酶从蔗糖中选择性地产生新颖的低聚糖例如烟草酵母和新的糖。
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公开(公告)号：KR1020080090855A
公开(公告)日：2008-10-09
申请号：KR1020070034231
申请日：2007-04-06
Applicant: 고려대학교 산학협력단
CPC classification number: Y02P20/544 , C12P7/649 , C12N9/20 , Y02E50/13
Abstract: A method for producing bio-diesel is provided to shorten reaction time and obtain the bio-diesel with high yield using lipase in supercritical fluid condition during an enzymatic process. In a method for producing bio-diesel using oils and fats and alcohol as substrates and lipase through an enzymatic process, the enzymatic process is performed in supercritical fluid condition under the pressure of 100-150 bar at a temperature of 40-60 deg.C at a stirring speed of 100-300 rpm, wherein the lipase uses 1,3-position specific lipase and a non-position specific lipase in a weight ratio of 1:3-3:1. Further, a supercritical fluid is a supercritical carbon dioxide.
Abstract translation: 提供了一种生产生物柴油的方法，以在酶处理过程中超临界流体条件下，利用脂肪酶缩短反应时间并以高产率获得生物柴油。 在使用油和脂肪和醇作为底物和通过酶促方法的脂肪酶生产生物柴油的方法中，酶法在超临界流体条件下在100-150巴的压力下在40-60℃的温度下进行 在100-300rpm的搅拌速度下，其中脂肪酶使用重量比为1:3-3:1的1,3-位特异性脂肪酶和非位置特异性脂肪酶。 此外，超临界流体是超临界二氧化碳。
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