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    미세결정 셀룰로오스 분해 활성을 갖는 갈색부후담자균포미톱시스 팔루스트리스에서 유래한 엔도글루카나제
    1.
    发明公开
    미세결정 셀룰로오스 분해 활성을 갖는 갈색부후담자균포미톱시스 팔루스트리스에서 유래한 엔도글루카나제 无效
    来自具有微晶纤维素降解活性的棕色基底肉芽孢杆菌的内源葡萄球菌

    公开(公告)号：KR1020090039972A

    公开(公告)日：2009-04-23

    申请号：KR1020070105496

    申请日：2007-10-19

    Applicant: 국민대학교산학협력단

    Inventor： 김영숙 ,  윤정준 ,  김영균 ,  차창준

    IPC: C12N15/31 ,  C12N15/52 ,  C12N1/20

    Abstract: An endoglucanase derived from fomitopsis palustris is provided to develop wood-biomass resolving system and manufacturing technique of wood-biomass lysis enzyme. An endoglucanase EG47 derivated from fomitopsis palustris contains amino sequence ALFGLMNEPH and AAGAWNYLLL, molecular weight is 47kDa. The endoglucanase has microcrystal cellulose lysis activity. Wood-biomass lysis enzyme comprises Endo-glucanase EG 47 or Endo-glucanase EG 35. The studying for the Endoglucanase comprises: a step of purifying glycosylhydrolase from fomitopsis palustris; a step of analyzing enzyme and concentrating protein; a step of identifying purified enzyme by analyzing amino sequence; a step of analyzing specificity of purified Endoglucanase; and a step of analyzing with chromatography.

    Abstract translation: 提供了一种来自瓢虫的内切葡聚糖酶，用于开发木材 - 生物质解析系统和木材 - 生物质裂解酶的制造技术。 来自瓢虫的内切葡聚糖酶EG47含有氨基酸序列ALFGLMNEPH和AAGAWNYLLL，分子量为47kDa。 内切葡聚糖酶具有微晶纤维素裂解活性。 木质生物质裂解酶包括内切葡聚糖酶EG47或内切葡聚糖酶EG 35.内切葡聚糖酶的研究包括：从番茄中提取糖基水解酶的步骤; 分析酶和浓缩蛋白的步骤; 通过分析氨基序列鉴定纯化酶的步骤; 分析纯化内切葡聚糖酶的特异性的步骤; 和用色谱法分析的步骤。

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