公开(公告)号：KR1020020091628A

公开(公告)日：2002-12-06

申请号：KR1020010030456

申请日：2001-05-31

Applicant: 국방과학연구소

Inventor： 최기봉 ,  하연철 ,  윤희주 ,  양진아

IPC: G01N33/537

Abstract: PURPOSE: Provided are competitive enzyme-linked immunofiltration assay using multi-filters and a kit therefor, thereby reducing time and cost for assay. CONSTITUTION: The competitive enzyme-linked immunofiltration assay method comprises the steps of: carrying out immune reaction of a liquid sample to be analyzed by adding an antibody-enzyme complex; removing an immune complex and other immune aggregations by passing a mixed solution containing an immune complex and unreacted antibody-enzyme complex through a membrane filter; carrying out immune reaction of the filtered unreacted antibody-enzyme on an inclusion membrane where the sample to be analyzed is fixed; and analyzing the presence and concentration of the sample to be analyzed by adding an enzyme specific substrate included in the immune complex.

Abstract translation: 目的：提供使用多过滤器和其试剂盒的竞争性酶联免疫过滤测定法，从而减少测定的时间和成本。 构成：竞争性酶联免疫过滤测定方法包括以下步骤：通过加入抗体 - 酶复合物进行待分析液体样品的免疫反应; 通过使含有免疫复合物和未反应的抗体 - 酶复合物的混合溶液通过膜过滤器去除免疫复合物和其它免疫聚集体; 在被分析样品固定的包覆膜上进行过滤的未反应抗体酶的免疫反应; 并通过添加包含在免疫复合物中的酶特异性底物来分析待分析样品的存在和浓度。

公开(公告)号：KR100436567B1

公开(公告)日：2004-06-18

申请号：KR1020010030456

申请日：2001-05-31

Applicant: 국방과학연구소

Inventor： 최기봉 ,  하연철 ,  윤희주 ,  양진아

IPC: G01N33/537

Abstract: PURPOSE: Provided are competitive enzyme-linked immunofiltration assay using multi-filters and a kit therefor, thereby reducing time and cost for assay. CONSTITUTION: The competitive enzyme-linked immunofiltration assay method comprises the steps of: carrying out immune reaction of a liquid sample to be analyzed by adding an antibody-enzyme complex; removing an immune complex and other immune aggregations by passing a mixed solution containing an immune complex and unreacted antibody-enzyme complex through a membrane filter; carrying out immune reaction of the filtered unreacted antibody-enzyme on an inclusion membrane where the sample to be analyzed is fixed; and analyzing the presence and concentration of the sample to be analyzed by adding an enzyme specific substrate included in the immune complex.

Abstract translation: 目的：提供使用多重过滤器及其试剂盒的竞争性酶联免疫过滤测定法，由此减少测定的时间和成本。 组成：竞争性酶联免疫渗滤分析方法包括以下步骤：通过加入抗体 - 酶复合物进行待分析液体样品的免疫反应; 通过使含有免疫复合物和未反应的抗体 - 酶复合物的混合溶液通过膜过滤器来去除免疫复合物和其他免疫聚集体; 在待分析样品固定的包含膜上进行过滤的未反应抗体 - 酶的免疫反应; 并通过加入包含在免疫复合物中的酶特异性底物来分析待分析样品的存在和浓度。