Abstract:
A kit for genetically discriminating ginseng species by restriction fragment length polymorphism(RFLP) methods is provided to improve reproducibility and accuracy of discrimination, and evaluate relationship and genetic mutations among ginseng species. The kit for genetically discriminating ginseng species by restriction fragment length polymorphism(RFLP) methods comprises a DNA extracting reagent, a PCR reagent containing sense and antisense primers and restriction enzymes, wherein the sense primer has the nucleotide sequence of SEQ ID NO:1 and the antisense primer has the nucleotide sequence of SEQ ID NO:2; and the restriction enzymes include Sau3AI capable of recognizing the nucleotide sequence of SEQ ID NO:3. A method for genetically discriminating ginseng species comprises the steps of: extracting DNA from a ginseng sample; preparing the reaction solution containing extracted DNA and sense and antisense primers; PCR-amplifying the reaction solution; performing the RFLP method with the amplified DNA product.
Abstract translation:提供了一种通过限制性片段长度多态性(RFLP)方法对人参进行遗传鉴定的试剂盒,以提高鉴别的重现性和准确性,并评估人参物种之间的关系和遗传突变。 通过限制性片段长度多态性(RFLP)方法用于遗传鉴别人参物种的试剂盒包括DNA提取试剂,含有义和反义引物和限制酶的PCR试剂,其中正义引物具有SEQ ID NO:1的核苷酸序列和 反义引物具有SEQ ID NO:2的核苷酸序列; 并且限制酶包括能识别SEQ ID NO:3的核苷酸序列的Sau3AI。 遗传上鉴别人参物种的方法包括以下步骤:从人参中提取DNA; 制备含有提取的DNA和有义和反义引物的反应溶液; PCR扩增反应溶液; 用扩增的DNA产物进行RFLP方法。
Abstract:
본 발명은 홍화자(Carthami Semen), 숙지황(Rehmanninae Rhizoma Preparat) 및 사인(Amomi fructus)의 혼합생약 추출물을 유효성분으로 함유하는 골다공증질환의 예방 및 치료용 약학 조성물에 관한 것으로, 본 발명의 혼합생약 추출물은 조골세포주인 MG-63세포의 생존율을 증가시킬 뿐만 아니라 골다공증유발 원인인 감소된 골(질)량, 에스트라디올(estradiol) 및 오스테오칼신(osteocalcin)을 증가시켜주는 효과를 나타내므로, 골다공증질환의 예방 및 치료용 약학 조성물 및 건강기능식품에 유용하게 이용될 수 있다. 홍화자, 숙지황, 사인, 골다공증질환
Abstract:
Disclosed is a pharmaceutical composition having an extract of curculigo orchioides for preventing or treating reflux esophagitis. The present invention provides the pharmaceutical composition having the extract of curculigo orchioides for preventing or treating reflux esophagitis and functional foods for preventing or alleviating reflux esophagitis.
Abstract:
A pharmaceutical composition for preventing and treating osteoporosis, which contains mixed herbal extract of Carthami Semen, Rehmanninae Rhizoma Preparat, and Amomi fructus is provided to increase the viability of MG-63 cell which is osteoblast cell line, MG-63 cell and increase estradiol and osteocalcin. A pharmaceutical composition for preventing and treating osteoporosis comprises mixed herbal extract of Carthami Semen, Rehmanninae Rhizoma Preparat and Amomi fructus as an active ingredient. The mixed herbal extract is isolated using water, low alcohol of 1-4 carbon atoms or their mixture solvent. The mixed herbal extract of Carthami Semen, Rehmanninae Rhizoma Preparat and Amomi fructus is contained in the weight ratio of 1-10:1-5:1-5.