Abstract:
The present invention relates to a method for distinguishing Epinephelus bruneu from Epinephelus septemfasciatus and a kit thereof which use a D-loop region of mitochondrial genome sequences. The method for distinguishing Epinephelus bruneu from Epinephelus septemfasciatus includes the steps of: (a) obtaining biological samples from Epinephelus bruneu and Epinephelus septemfasciatus; (b) separating mitochondrial genome DNAs of Epinephelus bruneu from mitochondrial genome DNAs of Epinephelus septemfasciatus; (c) amplifying the D-loop region of genome DNAs; and (d) identifying the length of an amplified product.