자바리와 능성어의 식별 방법 및 그 키트
    2.
    发明公开
    자바리와 능성어의 식별 방법 및 그 키트 有权
    用于分离EPINEPHELUS BRUNEU和EPINEPHELUS SEPTEMFASCIATUS及其制品的方法

    公开(公告)号:KR1020140093889A

    公开(公告)日:2014-07-29

    申请号:KR1020130038330

    申请日:2013-04-08

    CPC classification number: C12Q1/6806 C12N15/11 C12Q1/6811 C12Q1/6844

    Abstract: The present invention relates to a method for distinguishing Epinephelus bruneu from Epinephelus septemfasciatus and a kit thereof which use a D-loop region of mitochondrial genome sequences. The method for distinguishing Epinephelus bruneu from Epinephelus septemfasciatus includes the steps of: (a) obtaining biological samples from Epinephelus bruneu and Epinephelus septemfasciatus; (b) separating mitochondrial genome DNAs of Epinephelus bruneu from mitochondrial genome DNAs of Epinephelus septemfasciatus; (c) amplifying the D-loop region of genome DNAs; and (d) identifying the length of an amplified product.

    Abstract translation: 本发明涉及一种用于区分Ep蛇属和Ep蛇属的方法及其使用线粒体基因组序列的D环区的试剂盒。 鉴别Ep蛇属和Ep蛇属的方法包括以下步骤:(a)从Ep from和Ep皮瓣获得生物样品; (b)从Ep蛇线虫线粒体基因组DNA中分离Ep蛇属线粒体基因组DNA; (c)扩增基因组DNA的D环区域; 和(d)鉴定扩增产物的长度。

    감자난초와 두잎감자난초의 감별 방법 및 키트
    10.
    发明公开
    감자난초와 두잎감자난초의 감별 방법 및 키트 有权
    用于区分OREORCHIS PATENS和OREORCHIS COREANA之间的方法和工具包

    公开(公告)号:KR1020130022313A

    公开(公告)日:2013-03-06

    申请号:KR1020110085659

    申请日:2011-08-26

    CPC classification number: C12Q1/683 C12Q1/6858 C12Q2600/13 C12Q2600/156

    Abstract: PURPOSE: A method and a kit for discriminating Oreorchis patens and Oreorchis coreana Finet are provided to easily discriminate the Oreorchis patens and the Oreorchis coreana Finet by using a direct sequencing method, an SNP-genotyping method, and an RFLP(Restriction fragment length polymorphism) method. CONSTITUTION: A method for discriminating Oreorchis patens and Oreorchis coreana Finet comprises: a step of preparing biological samples from the Oreorchis patens and the Oreorchis coreana Finet; a step of isolating genome DNA from the biological samples; a step of amplifying a DNA sequence containing a GCCGGC sequence, which is a recognition site of a Nael restriction enzyme in an ITS sequence of the Oreorchis coreana Finet; a step of amplifying a DNA sequence containing a GCNGGC sequence, which is an alternative sequence of the Oreorchis patens; and a step of treating the amplified product with NaeI.

    Abstract translation: 目的:提供一种用于区分Oreorchis patens和Oreorchis coreana Finet的方法和试剂盒,以便通过使用直接测序方法,SNP基因分型法和RFLP(限制性片段长度多态性)轻松区分Oreorchis patens和Oreorchis coreana Finet, 方法。 构成:一种鉴别Oreorchis patens和Oreorchis coreana Finet的方法包括:从Oreorchis patens和Oreorchis coreana Finet制备生物样品的步骤; 从生物样品中分离基因组DNA的步骤; 在Oreorchis coreana Finet的ITS序列中扩增含有作为Nael限制酶的识别位点的GCCGGC序列的DNA序列的步骤; 扩增含有作为Oreorchis patens的替代顺序的GCNGGC序列的DNA序列的步骤; 以及用NaeI处理扩增产物的步骤。

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