Abstract:
The present invention relates to a method for distinguishing Epinephelus bruneu from Epinephelus septemfasciatus and a kit thereof which use a D-loop region of mitochondrial genome sequences. The method for distinguishing Epinephelus bruneu from Epinephelus septemfasciatus includes the steps of: (a) obtaining biological samples from Epinephelus bruneu and Epinephelus septemfasciatus; (b) separating mitochondrial genome DNAs of Epinephelus bruneu from mitochondrial genome DNAs of Epinephelus septemfasciatus; (c) amplifying the D-loop region of genome DNAs; and (d) identifying the length of an amplified product.
Abstract:
본 발명은 감자난초 및 두잎감자난초의 감별 방법 및 그 키트에 관한 것이다. 본 발명의 감자난초 및 두잎감자난초의 감별 방법 및 그 키트는 두잎감자난초의 ITS 서열에 존재하는 Nae I 제한효소 인식 부위와 이에 대한 감자난초의 대립 서열을 이용하거나 감자난초와 두잎감자난초의 ITS 서열의 SNP 마커를 이용함을 특징으로 한다.
Abstract:
PURPOSE: A method and a kit for discriminating Oreorchis patens and Oreorchis coreana Finet are provided to easily discriminate the Oreorchis patens and the Oreorchis coreana Finet by using a direct sequencing method, an SNP-genotyping method, and an RFLP(Restriction fragment length polymorphism) method. CONSTITUTION: A method for discriminating Oreorchis patens and Oreorchis coreana Finet comprises: a step of preparing biological samples from the Oreorchis patens and the Oreorchis coreana Finet; a step of isolating genome DNA from the biological samples; a step of amplifying a DNA sequence containing a GCCGGC sequence, which is a recognition site of a Nael restriction enzyme in an ITS sequence of the Oreorchis coreana Finet; a step of amplifying a DNA sequence containing a GCNGGC sequence, which is an alternative sequence of the Oreorchis patens; and a step of treating the amplified product with NaeI.