Abstract:
PURPOSE: A feminization inducing method of cultured eel is provided to induce feminization by injecting 17 β - estradiol in elver step, and to use on a production technology of female brood fish for spawning induction which is needed for artificial seeding production technology of eel. CONSTITUTION: A feminization inducing method of cultivated eel comprises the steps of: injecting 10-25 mg of 17 β - estradiol per 1 kg of the fodder into elvers for 7 months; injecting the 17 β - estradiol for 5 months; and injecting the 17 β - estradiol on alternate days. The average weight of the elvers is 0.15-0.2 g, and the average total length is 5-6 cm.
Abstract:
The present invention relates to a donut-shaped water tank for an eel larva mass production having an integrated type water supply/drain pipe. The provided donut-shaped water tank is equipped with a feeding water rotation supply spray device which circulates at an appropriate flow rate so that larvae meet liquid feed in the tank which has a large diameter capable of accommodating the larvae in large quantities. Thus, the present invention reduces labor costs and prevents the mortality of larva by not only evenly mixing the liquid feed without forming a blind spot which is formed in an existing water tank when spraying feeding water or air but also automatically circulating the liquid feed in the water tank by controlling a feeding water spray device and the rotation speed of a sub motor for mixing the liquid feed. Also, the present invention can easily disassemble and assemble each component of a water tank main body, drain equipment, and the feeding water spray device which compose the water tank.
Abstract:
The present invention provides an incubation system for fish larva which automatically mixes a liquid feed in an aquaculture tank at a proper flow rate to increase the survival rate of early-stage larval eels and to manage the early-stage larval eels in large amounts. The circulation rate and location of water is adjusted to exact figures which do not impose stress on the early-stage larval eels for an increased survival rate and mass production of the early-stage larval eels.
Abstract:
A method for diagnosis of maturity by using a polyclonal antibody of rockfish(Sebastes schlegeli) is provided to improve diagnosis accuracy of rockfish maturity by measuring the amount of luteinizing hormone(LH) in rockfish. The method for diagnosis of maturity by using a polyclonal antibody of rockfish(Sebastes schlegeli) comprises the steps of: collecting the pituitary gland of rockfish and purifying it to obtain the rockfish luteinizing hormone; separating rockfish glycoprotein alpha(GPalpha) and rockfish luteinizing hormone beta(LHbeta) from the rockfish luteinizing hormone, and independently obtaining polyclonal antibodies against the rockfish glycoprotein alpha(GPalpha) and rockfish luteinizing hormone beta(LHbeta); and deciding maturity of rockfish through enzyme-linked immunoassay method by using the polyclonal antibody against rockfish luteinizing hormone beta(LHbeta).
Abstract:
본 발명은 우리나라의 주요 양식 어종인 조피볼락의 뇌하수체에서 분리한 생식선자극호르몬의 3가지 서브 유니트 유전자(GTHα, FSHβ, LHβ)의 염기서열을 제공하여 조피볼락의 생식선자극호르몬의 cDNA들의 성성숙을 유도하거나, 우수한 난질을 가진 난모세포의 획득 및 우량종묘생산을 위한 생리활성물질 생산에 유용하게 이용 가능함으로써 국내 양식산업의 고부가가치를 창출함은 물론, 어류로부터 분리한 유용 유전자의 산업화로 어류 유래 성성숙유도 호르몬제 개발에 기여할 뿐만 아니라, 조피볼락에 있어 생식선자극호르몬 유전자의 제어영역과 조절인자를 밝혀 여포자극호르몬 및 황체형성호르몬의 결손 및 과잉발현을 밝혀낼 수 있는 요인을 제공할 수 있도록 하는 조피볼락 뇌하수체 유래의 당단백질성 생식선자극호르몬의 서브 유니트 유전자 염기서열에 관한 것이다. 본 발명은 서열 1, 2, 3에 기재된 염기서열로 표시되는 조피볼락 뇌하수체 유래의 당단백질성 생식선자극호르몬의 서브 유니트 염기서열에 특징이 있다. 조피볼락, 유전자, 염기서열, 생식선자극호르몬, 황체형성호르몬, 여포자극호르몬