公开(公告)号：KR1020140045774A

公开(公告)日：2014-04-17

申请号：KR1020120111953

申请日：2012-10-09

Applicant: 전북대학교산학협력단 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김원일 ,  최은진 ,  김은주 ,  이창희 ,  송재영

IPC: C12N7/01 ,  A61K39/12 ,  G01N33/569

Abstract: The present invention relates to a porcine reproductive and respiratory syndrome virus and a vaccine comprising the same for preventing and treating porcine reproductive and respiratory syndrome. PRRSV according to the present invention which an ORF (open reading frame) 5 or ORF 7 of CB11054 increase a production of a cytokine like TNF-alpha, INF-alpha, INF-beta, and IL-12, to have an excellent effect of inducing an immune response, therefore, has an excellent defense capabilities against PRRSV after vaccination.

Abstract translation: 本发明涉及一种猪繁殖与呼吸综合征病毒和一种包含其的预防和治疗猪繁殖与呼吸综合征的疫苗。 根据本发明的PR1V，CB11054的ORF（开放阅读框）5或ORF 7增加细胞因子如TNF-α，INF-α，INF-β和IL-12的产生，具有优异的 因此，诱导免疫应答在接种疫苗后对PRRSV具有极好的防御能力。

公开(公告)号：KR101430223B1

公开(公告)日：2014-09-25

申请号：KR1020120111953

申请日：2012-10-09

Applicant: 전북대학교산학협력단 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김원일 ,  최은진 ,  김은주 ,  이창희 ,  송재영

IPC: C12N7/01 ,  A61K39/12 ,  G01N33/569

Abstract: 본 발명은 면역유도능이 증가된 돼지생식기호흡기증후군 바이러스 및 이를 포함하는 돼지생식기호흡기 증후군 예방 또는 치료용 백신 조성물에 관한 것이다. 본 발명에 따른 CB11054의 ORF(open reading frame) 5 또는 ORF 7이 도입된 PRRSV는 TNF-α, INF-α, INF-β 및 IL-12 등 사이토카인의 생산을 증가시켜 면역 반응을 유도하는 효과가 우수하므로, 백신 접종 시 PRRSV에 대한 방어능이 우수하다.

公开(公告)号：KR100430935B1

公开(公告)日：2004-05-14

申请号：KR1020010082975

申请日：2001-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  권병준 ,  고영준 ,  나진주 ,  김원일 ,  이세영 ,  주이석

IPC: C12N15/33

Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.

Abstract translation: 目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基​​因具有SEQ ID NO：1的核苷酸序列。重组3ABC非结构蛋白由重组 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E.coli中表达的重组3ABC非结构蛋白制备杂交瘤细胞系3F-11（KCTC 10138BP） 将大肠杆菌导入动物体内，从动物体内收集免疫细胞，并将免疫细胞与癌细胞融合。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫诊断方法包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入板中; （2）使测试血清与平板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，与偶联物的荧光反应或辐射反应。

公开(公告)号：KR1020030052857A

公开(公告)日：2003-06-27

申请号：KR1020010082975

申请日：2001-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  권병준 ,  고영준 ,  나진주 ,  김원일 ,  이세영 ,  주이석

IPC: C12N15/33

CPC classification number: C07K14/005 ,  C07K16/1009 ,  C07K2317/14 ,  C12N5/163 ,  C12N15/70 ,  C12N2770/32131 ,  G01N33/56983 ,  G01N33/577 ,  G01N2333/09

Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.

Abstract translation: 目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫的诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基​​因具有SEQ ID NO：1的核苷酸序列。重组体3ABC非结构蛋白由重组体 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E中表达的重组3ABC非结构蛋白来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 将大肠杆菌转化成动物，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，并将稀释液倒在平板上; （2）使测试血清与板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，荧光反应或与缀合物的辐射反应。