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公开(公告)号：KR101207213B1
公开(公告)日：2012-12-13
申请号：KR1020090114269
申请日：2009-11-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/10 , C12N15/12 , C12N15/867
CPC classification number: C12N7/00 , C12N2740/15043 , C12N2790/10051
Abstract: 본발명은전염성해면상뇌증의전염성해면상뇌증(TSE; transmissible spongiform encephalopathy) 감수성세포의제조방법및 그방법에의해서수득된감수성세포의활용에관한것으로, 본발명에의해서수득되는전염성해면상뇌증감수성세포는전염성해면상뇌증의진단, 병인론및 신약개발등에유리하게활용될수 있다.
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公开(公告)号：KR101077188B1
公开(公告)日：2011-10-31
申请号：KR1020080117663
申请日：2008-11-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은구제역바이러스억제및 예방을목적으로특이적으로제작된 siRNA를발현하는재조합아데노바이러스에관한것이다. 보다상세하게는, 우리나라에발생하였던구제역바이러스주(O/SKR/2002)의염기서열을바탕으로비구조단백질부위의특이염기서열을찾고이를디자인한후 아데노클로닝벡터에클로닝하여제조한유전자재조합아데노바이러스에관한것으로서, 이를동물이바이러스에감염되기전에적용하면, 바이러스억제효과를나타내며, 동물이바이러스에감염된이후에적용하더라도바이러스의증식억제효과를나타내어구제역의예방과치료에모두활용할수 있다.
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公开(公告)号：KR1020040073034A
公开(公告)日：2004-08-19
申请号：KR1020030008750
申请日：2003-02-12
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K7/08
CPC classification number: C07K7/08 , G01N33/56983 , G01N2333/115
Abstract: PURPOSE: A peptide specifically reactive with antibodies against rinderpest virus and uses thereof are provided, thereby reducing the detection time and improving detection activity of rinderpest virus antibodies by using the peptide with bovine serum albumin. CONSTITUTION: The peptide specifically reactive with antibodies against rinderpest virus has the amino acid sequence set forth in SEQ ID NO:1, wherein the peptide further contains cysteine in the N-terminal and amino group(-NH2) in the C-terminal. A composition for detecting antibodies against rinderpest virus contains the peptide of SEQ ID NO:1. A kit for detecting antibodies against rinderpest virus contains the peptide of SEQ ID NO:1.
Abstract translation: 目的：提供与抗牛瘟病毒抗体特异性反应的肽及其用途，通过使用牛血清白蛋白的肽，减少检测时间，提高牛瘟病毒抗体检测活性。 构成：与牛瘟病毒抗体特异性反应的肽具有SEQ ID NO：1所示的氨基酸序列，其中肽在N-末端还含有C-末端的氨基（-NH 2）。 用于检测针对牛瘟病毒的抗体的组合物含有SEQ ID NO：1的肽。 用于检测针对牛瘟病毒的抗体的试剂盒含有SEQ ID NO：1的肽。
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4.发明公开
公开(公告)号：KR1020030052858A
公开(公告)日：2003-06-27
申请号：KR1020010082976
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N15/866 , C07K14/005 , C07K16/1009 , C07K2317/14 , C12N5/163 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在昆虫细胞中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组体3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒通过 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。重组体3ABC非结构蛋白用重组杆状病毒感染昆虫细胞表达。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白质导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，将稀释液倒入平板上; （2）使测试血清与板反应; （3）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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公开(公告)号：KR101149396B1
公开(公告)日：2012-06-27
申请号：KR1020090067453
申请日：2009-07-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 사슴의 PrP 펩타이드 중 특정부위를 선발하여 이를 유전자 적중 마우스에 접종하여 면역시킨 후 여기에서 분리한 면역 B 세포와 마우스 골수종유래세포를 융합하여 제조한 융합세포주; 상기 융합세포주로부터 생산되고 사슴만성소모성질병의 변형프리온(PrP
sc )을 사슴의 정상프리온 및 소해면상뇌증 및 스크래피(Scrapie) 등의 다른 전염성해면상뇌증의 변형프리온과 감별하여 검출해 내는 단클론항체 및 그 용도에 관한 것이다.
사슴만성소모성질병, 변형프리온, 단클론항체, 융합세포주, PrP 펩타이드, 전염성해면상뇌증-
Patent Agency Ranking
公开(公告)号：KR1020110057733A
公开(公告)日：2011-06-01
申请号：KR1020090114269
申请日：2009-11-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N5/10 , C12N15/12 , C12N15/867
CPC classification number: C12N7/00 , C12N2740/15043 , C12N2790/10051
Abstract: PURPOSE: A method for preparing TSE(transmissible spongiform encephalopathy) sensitive cells is provided to develop novel drugs. CONSTITUTION: A method for preparing TSE-sensitive cells comprises: a step of isolating normal prion protein gene from the brain of an animal which infected by TSE and cloning; a step of inserting the gene to a lentivirus transfer vector to prepare infectious recombinant lentivirus; and a step of inoculating the infectious recombinant lentivirus to non-animal mammal cell line to prepare transformed cells which express a normal prion protein.
Abstract translation: 目的：提供一种制备TSE（传染性海绵状脑病）敏感细胞的方法开发新药。 构成：用于制备TSE敏感细胞的方法包括：从TSE感染的动物的脑中分离正常的朊病毒蛋白基因和克隆的步骤; 将该基因插入慢病毒转移载体以制备感染性重组慢病毒的步骤; 以及将感染性重组慢病毒接种到非动物哺乳动物细胞系以制备表达正常朊病毒蛋白的转化细胞的步骤。
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公开(公告)号：KR100531760B1
公开(公告)日：2005-11-29
申请号：KR1020030026809
申请日：2003-04-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 프린스톤바이오메디테크코포레이션 , (주)피비엠이스트
Inventor： 조인수 , 현방훈 , 이광녕 , 엄재구 , 계수정 , 고영준 , 구복경 , 주이석 , 안수환 , 김인중 , 김옥경 , 김희정 , 장기용 , 신남규 , 황서하 , 강제모 , 김창호 , 고송우
IPC: G01N33/543
CPC classification number: G01N33/56983 , G01N33/526 , G01N33/558 , G01N2333/09 , Y10S436/811
Abstract: 본 발명은 (1) 검체를 스트립의 로딩영역에 일정량 투입하는 단계; (2) 소정의 표지가 구비된 검출시약과 상기 검체 중의 분석물과 결합시켜 복합체를 형성하는 단계; (3) 상기 복합체를 전개막에 전개하는 단계; 및 (4) 상기 전개막 상의 소정 영역에 FMDV로부터 유래된 또는 FMDV로부터 면역반응을 통해 얻어질 수 있는 항원, 항체 또는 합텐 중에서 선택되는 적어도 하나 이상의 고정상을 갖는 반응부의 외관 변화를 관찰하여 구제역 바이러스의 감염여부를 판단하는 단계를 포함하는 구제역 바이러스 감염 진단방법을 제공한다.
또한 본 발명은 상기 진단방법을 구현하기 위한 진단 키트로서, FMDV로부터 유래된 또는 FMDV로부터 면역반응을 통해 얻어질 수 있는 항원, 항체 또는 합텐 중에서 선택되는 적어도 하나 이상의 고정상을 갖는 반응부(13) 및 정상동작여부를 판단하기 위한 대조부(14)가 전개막(9)상의 소정영역에 구비된 스트립(1); 상기 스트립(1)을 각종 오염물질로부터 보호하며, 적어도 검체를 투입하기 위한 검체투입부(2) 및 스트립상의 반응부(13)와 대조부(14)에서의 반응결과를 관찰하기 위한 표시창(4)이 구비된 하우징(20)을 포함하는 구제역 바이러스 감염 진단용 키트를 제공한다.-
公开(公告)号：KR100522086B1
公开(公告)日：2005-11-09
申请号：KR1020010082715
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/68
Abstract: 본 발명은 아프리카 마역 바이러스를 검출하여, 아프리카 마역을 진단하는 방법에 관한 것으로, 더욱 상세하게는 아프리카 마역바이러스에 감염된 혈청 내에 존재하는 VP7에 대한 항체를 아프리카 마역바이러스의 VP7 항원 및 상기 VP7 항원에 대한 모노클로날 항체를 이용하는 ELISA법으로 아프리카 마역 항체를 검출함으로써, 아프리카 마역을 진단하는 방법에 관한 것이다.
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9.发明授权수포성 구내염 바이러스의 외피당단백질 항원 및 그에대한 모노클로날 항체를 이용하는 수포성 구내염의 진단방법 有权수포성구내염바이러스의외피당단백질항원및그에대한모노클로날항체를이용하는수포성구내염의진단방
公开(公告)号：KR100449908B1
公开(公告)日：2004-09-22
申请号：KR1020010082714
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/12
Abstract: PURPOSE: A method of diagnosing vesicular stomatitis by performing an ELISA method using the recombinant envelope glycoprotein antigen of vesicular stomatitis virus(VSV) and monoclonal antibodies thereon is provided. Therefore, it permits quick, exact and specific antibody detection of the VSV and thus exact diagnosis of vesicular stomatitis through the detection. CONSTITUTION: The diagnosing method for vesicular stomatitis is achieved through a VSV detection method consisting of: attaching monoclonal antibodies against an envelope glycoprotein antigen of VSV on a solid supporter; binding the envelope glycoprotein antigen to the monoclonal antibodies attached on the solid supporter in the first step; reacting a sample containing an antibody against the envelope glycoprotein antigen with the envelope glycoprotein antigen of the second step; reacting a secondary antibody selected from the group consisting of a conjugate antibody attached with enzyme and a conjugate antibody attached with a luminescent material with the antibody of the third step.
Abstract translation: 目的：提供一种使用水疱性口炎病毒（VSV）的重组包膜糖蛋白抗原和单克隆抗体进行ELISA方法来诊断水疱性口炎的方法。 因此，它可以快速，准确和特异地检测VSV的抗体，从而通过检测来精确诊断水疱性口炎。 组成：水泡性口炎的诊断方法通过VSV检测方法实现，该方法包括：将抗VSV的包膜糖蛋白抗原的单克隆抗体附着在固体支持物上; 在第一步中将包膜糖蛋白抗原结合至附着于固体载体上的单克隆抗体; 使含有抗包膜糖蛋白抗原的抗体的样品与第二步的包膜糖蛋白抗原反应; 使选自附着有酶的缀合物抗体和附着有荧光物质的缀合物抗体的第二抗体与第三步骤的抗体反应。
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公开(公告)号：KR100449906B1
公开(公告)日：2004-09-22
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组载体通过克隆编码 重组FMCV 2C非结构蛋白SEQ ID NO：1。通过用重组载体转化细胞表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组FMCV 2C非结构蛋白特异性单克隆抗体稀释于包被缓冲液中，并将稀释液倒入平板中; （2）清洗平板以去除未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与平板反应; （4）洗涤平板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与平板反应; （6）洗涤平板以除去未反应的测试血清; （7）使与试验血清中的口蹄疫病毒抗体结合的缀合物与试验血清具有酶，放射性物质或荧光物质的步骤; 和（8）测量酶反应的强度，与缀合物的荧光反应或辐射反应。
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