公开(公告)号：KR1020100121289A

公开(公告)日：2010-11-17

申请号：KR1020090040369

申请日：2009-05-08

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 송재영 ,  임성인 ,  김재조 ,  조인수 ,  김병한 ,  한규하 ,  박길순

IPC: C12N7/01 ,  C12N15/33 ,  A61K39/12

Abstract: PURPOSE: A recombinant Flc-LOM swine fever vaccine virus is provided to easily detect other vaccine virus and outdoor virus and to early detect infected pig. CONSTITUTION: A vaccine virus for swine fever, Flc-LOM virus(KFCC11441P) has a nucleotide sequence of sequence number 1. In the vaccine virus, 52, 96, 220, 405th bases are changed from C, A, G, and G to T, G, T, and A. The vaccine virus Flc-LOM genetic marker or END(Exaltaton of Newcastle Disease virus). A live vaccine for swine fever contains the vaccine virus Flc-LOM virus(KFCC 11441P).

Abstract translation: 目的：提供重组Flc-LOM猪瘟疫苗病毒，轻松检测其他疫苗病毒和户外病毒，及早发现感染猪。 构成：用于猪瘟的疫苗病毒Flc-LOM病毒（KFCC11441P）具有序列号1的核苷酸序列。在疫苗病毒中，52,96,220,405碱基从C，A，G和G改变为 T，G，T和A.疫苗病毒Flc-LOM遗传标记或END（新城疫病毒）。 用于猪瘟的活疫苗包含疫苗病毒Flc-LOM病毒（KFCC 11441P）。

公开(公告)号：KR1020100121288A

公开(公告)日：2010-11-17

申请号：KR1020090040368

申请日：2009-05-08

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 송재영 ,  임성인 ,  김재조 ,  조인수 ,  한규하 ,  박길순 ,  이오수

IPC: C12N7/01 ,  C12N15/33 ,  A61K39/12

Abstract: PURPOSE: A vaccine virus for swine fever of gene recombination is provided to easily detect mutation of vaccine virus and to early remove infected pig. CONSTITUTION: A vaccine virus for swine fever, Flc-LOM-BErns virus(KFCC11442P) contains a nucleotide of sequence number 1. An Erns gene of CSFV(classical swine fever virus) is substituted with Erns gene of BVDV(bovine viral disease virus) in the vaccine virus. The vaccine virus is Erns gene marker, Erns serologic marker or END(Exaltaton of Newcastle Disease virus) bio marker. A method for manufacturing swine vaccine virus, Flc-LOM-BErns virus(KFCC11442P) comprises: a step of extracting genomic RNA from swine fever virus strain; a step of synthesizing full-length cDNA to total RNA; a step of removing Erns gene from the synthesized cDNA; and a step of inserting Erns gene of BVDV.

Abstract translation: 目的：提供用于猪瘟基因重组的疫苗病毒，以便于检测疫苗病毒的突变并及早清除感染猪。 构成：用于猪瘟的疫苗病毒Flc-LOM-BErns病毒（KFCC11442P）含有序列号1的核苷酸。将CSFV（经典猪瘟病毒）的Erns基因用BVDV（牛病毒病毒）的Erns基因取代， 在疫苗病毒中。 疫苗病毒是Erns基因标记，Erns血清学标记或END（新城疫病毒Exaltaton）生物标志物。 一种制造猪疫苗病毒Flc-LOM-BErns病毒（KFCC11442P）的方法包括：从猪瘟病毒株中提取基因组RNA的步骤; 将全长cDNA合成到总RNA中的步骤; 从合成的cDNA中除去Erns基因的步骤; 和插入BVDV的Erns基因的步骤。