낭충봉아부패병을 포함한 꿀벌의 전염성 질병의 예방 및 치료를 위한 이산화염소 기체 소독장치
    1.
    发明公开
    낭충봉아부패병을 포함한 꿀벌의 전염성 질병의 예방 및 치료를 위한 이산화염소 기체 소독장치 有权
    生产二氧化氯气体的方法和方法,用于预防和治疗含有沙门氏菌的蜂蜜颗粒病

    公开(公告)号:KR1020130049377A

    公开(公告)日:2013-05-14

    申请号:KR1020110114369

    申请日:2011-11-04

    CPC classification number: A01K51/00 A61L2/20 A61L2202/11

    Abstract: PURPOSE: A disinfecting apparatus of chlorine dioxide gas is provided to prevent or treat sacbrood which a honey bee viral transmittable disease. CONSTITUTION: A disinfecting apparatus of chlorine dioxide comprises: a holder(100); a print circuit substrate(200) which is mounted at the side of the holder and has a UV LED; a power supply unit(300) which applies power to the print circuit substrate; and a medicine receiving unit(400) which is mounted at the holder and contained stabilized chlorine dioxide. The stabilized chlorine dioxide is gel, liquid, or solid phase. The power supply unit has an AC to DC converter for applying a current power of 3-9 bolts to the print circuit substrate.

    Abstract translation: 目的:提供二氧化氯气体的消毒装置,以防止或治疗蜜蜂病毒可传播疾病的囊袋。 构成:二氧化氯消毒装置包括:保持器(100); 印刷电路基板(200),其安装在所述保持件的侧面并具有UV LED; 电源单元(300),其向所述打印电路基板施加电力; 和药物接收单元(400),其安装在保持器上并且包含稳定的二氧化氯。 稳定的二氧化氯是凝胶,液体或固相。 电源单元具有AC-DC转换器,用于将3-9个螺栓的当前功率施加到印刷电路基板。

    돼지콜레라 바이러스 Erns 단백질 및 이에 특이적으로반응하는 단클론항체를 이용한 돼지콜레라 항체 감별진단방법
    5.
    发明公开
    돼지콜레라 바이러스 Erns 단백질 및 이에 특이적으로반응하는 단클론항체를 이용한 돼지콜레라 항체 감별진단방법 有权
    SWINE FEVER VIRUS ERNS蛋白质和筛选和检测与其相似的SWINE FEVER病毒抗体的方法

    公开(公告)号:KR1020070116382A

    公开(公告)日:2007-12-10

    申请号:KR1020060050357

    申请日:2006-06-05

    Abstract: A method for screening and detecting a swine fever virus antibody is provided to easily screen and diagnose infected swine fever virus antibody by using a monoclonal antibody specifically reacting with a recombinant swine fever virus Erns protein and secure constant sensitivity regardless of all genotypes of the swine fever virus when using all three kinds of recombinant protein. A recombinant swine fever virus Erns is prepared by expressing a gene encoding three Erns proteins among swine fever viruses having various genotypes using baculovirus. A method for preparing a monoclonal antibody specifically reacting to the Erns comprises the steps of: (a) constructing a swine fever virus LOM strain-derived Erns recombinant gene using a baculovirus expression system; and (b) producing the monoclonal antibody using the recombinant Erns protein. A method for screening and detecting a swine fever virus antibody comprises the steps of: (a) mixing three kinds of Erns recombinant proteins at proper concentration to prepare an antigen cocktail; (b) after diluting the antigen cocktail into a coating buffer solution, pouring it in a plate to be absorbed into the plate; (c) washing the recombinant antigen not-absorbed into the plate to remove it; (d) adding a sample to be tested to the plat to allow the reaction; (e) washing the sample to be tested not specifically bound to the recombinant Erns antigen protein absorbed into the plate to remove it; (f) adding a monoclonal antibody not being bound to the enzyme but being specifically reacted to the Erns protein to allow the reaction; (g) washing the monoclonal antibody not being bound to the recombinant Erns antigen protein absorbed into the plate to remove it; and (h) adding a substrate reacting with the enzyme to determine the swine fever virus infection.

    Abstract translation: 提供了一种筛选和检测猪瘟病毒抗体的方法,通过使用与重组猪瘟病毒Erns蛋白特异性反应的单克隆抗体轻松筛选和诊断感染的猪瘟病毒抗体,无论猪瘟的所有基因型如何 使用全部三种重组蛋白的病毒。 通过使用杆状病毒在具有各种基因型的猪瘟病毒中表达编码三种Erns蛋白质的基因来制备重组猪瘟病毒Erns。 制备与Erns特异性反应的单克隆抗体的方法包括以下步骤:(a)使用杆状病毒表达系统构建猪瘟病毒LOM菌株衍生的Erns重组基因; 和(b)使用重组Erns蛋白产生单克隆抗体。 筛选和检测猪瘟病毒抗体的方法包括以下步骤:(a)以适当浓度混合三种Erns重组蛋白以制备抗原混合物; (b)将抗原混合物稀释到涂布缓冲溶液中后,将其倒入板中吸收到板中; (c)将未吸收的重组抗原洗涤到板中以除去它; (d)将待测试样品加入平板以允许反应; (e)洗涤待测试的样品,未特异性地结合到吸收到板中的重组Erns抗原蛋白质以除去它; (f)加入未结合酶的单克隆抗体,但与Erns蛋白特异性反应以进行反应; (g)洗涤未结合到吸附到板中的重组Erns抗原蛋白的单克隆抗体以除去它; 和(h)加入与酶反应的底物以确定猪瘟病毒感染。

    낭충봉아부패병을 포함한 꿀벌의 전염성 질병의 예방 및 치료를 위한 이산화염소 기체 소독장치
    6.
    发明授权
    낭충봉아부패병을 포함한 꿀벌의 전염성 질병의 예방 및 치료를 위한 이산화염소 기체 소독장치 有权
    一种生产二氧化氯气体的装置和方法,用于预防和治疗含有sacbrood的蜂蜜蜂病

    公开(公告)号:KR101315260B1

    公开(公告)日:2013-10-10

    申请号:KR1020110114369

    申请日:2011-11-04

    Abstract: 본 발명의 낭충봉아부패병을 포함한 꿀벌의 전염성 질병의 예방 및 치료를 위한 이산화염소 기체 소독장치는, 양봉용 벌통에 설치되는 이산화염소 기체 소독장치에 있어서, 거치대; 상기 거치대 내 측면에 설치되며 자외선 LED가 설치된 인쇄회로기판; 상기 인쇄회로기판에 전원을 인가하는 전원부; 및 상기 자외선 LED와 대면하는 부위의 거치대에 설치되며 안정화이산화염소가 수납된 약제수납부;를 포함하는 것을 특징으로 한다.
    이에 따라, 안정화이산염소에 자외선을 조사하여 발생된 공기보다 비중이 무거운 이산화염소기체를 벌통에 주입하여 꿀벌(bee)의 유충 및 성충에서 발생되는 감염성 전염병의 대표적 사례인 꿀벌의 바이러스성 전염성 질병인 낭충봉아부패병(sacbrood)을 예방 또는 치료하는 효과가 있다.

    유전자재조합 돼지열병 백신바이러스 Flc―LOM―BErns virus 및 이의 제조방법
    8.
    发明公开
    유전자재조합 돼지열병 백신바이러스 Flc―LOM―BErns virus 및 이의 제조방법 无效
    遗传重组人兽毒病毒感染者FLC-LOM-BERNS病毒及其制备方法

    公开(公告)号:KR1020100121288A

    公开(公告)日:2010-11-17

    申请号:KR1020090040368

    申请日:2009-05-08

    Abstract: PURPOSE: A vaccine virus for swine fever of gene recombination is provided to easily detect mutation of vaccine virus and to early remove infected pig. CONSTITUTION: A vaccine virus for swine fever, Flc-LOM-BErns virus(KFCC11442P) contains a nucleotide of sequence number 1. An Erns gene of CSFV(classical swine fever virus) is substituted with Erns gene of BVDV(bovine viral disease virus) in the vaccine virus. The vaccine virus is Erns gene marker, Erns serologic marker or END(Exaltaton of Newcastle Disease virus) bio marker. A method for manufacturing swine vaccine virus, Flc-LOM-BErns virus(KFCC11442P) comprises: a step of extracting genomic RNA from swine fever virus strain; a step of synthesizing full-length cDNA to total RNA; a step of removing Erns gene from the synthesized cDNA; and a step of inserting Erns gene of BVDV.

    Abstract translation: 目的:提供用于猪瘟基因重组的疫苗病毒,以便于检测疫苗病毒的突变并及早清除感染猪。 构成:用于猪瘟的疫苗病毒Flc-LOM-BErns病毒(KFCC11442P)含有序列号1的核苷酸。将CSFV(经典猪瘟病毒)的Erns基因用BVDV(牛病毒病毒)的Erns基因取代, 在疫苗病毒中。 疫苗病毒是Erns基因标记,Erns血清学标记或END(新城疫病毒Exaltaton)生物标志物。 一种制造猪疫苗病毒Flc-LOM-BErns病毒(KFCC11442P)的方法包括:从猪瘟病毒株中提取基因组RNA的步骤; 将全长cDNA合成到总RNA中的步骤; 从合成的cDNA中除去Erns基因的步骤; 和插入BVDV的Erns基因的步骤。

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