公开(公告)号：KR101207213B1

公开(公告)日：2012-12-13

申请号：KR1020090114269

申请日：2009-11-25

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 탁동섭 ,  김효진 ,  손현주 ,  이윤희 ,  김민정 ,  윤은임 ,  조인수 ,  권창희 ,  주이석 ,  오토윈들 ,  마이클닐

IPC: C12N5/10 ,  C12N15/12 ,  C12N15/867

CPC classification number: C12N7/00 ,  C12N2740/15043 ,  C12N2790/10051

Abstract: 본발명은전염성해면상뇌증의전염성해면상뇌증(TSE; transmissible spongiform encephalopathy) 감수성세포의제조방법및 그방법에의해서수득된감수성세포의활용에관한것으로, 본발명에의해서수득되는전염성해면상뇌증감수성세포는전염성해면상뇌증의진단, 병인론및 신약개발등에유리하게활용될수 있다.

公开(公告)号：KR1020110057733A

公开(公告)日：2011-06-01

申请号：KR1020090114269

申请日：2009-11-25

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 탁동섭 ,  김효진 ,  손현주 ,  이윤희 ,  김민정 ,  윤은임 ,  조인수 ,  권창희 ,  주이석 ,  오토윈들 ,  마이클닐

IPC: C12N5/10 ,  C12N15/12 ,  C12N15/867

CPC classification number: C12N7/00 ,  C12N2740/15043 ,  C12N2790/10051

Abstract: PURPOSE: A method for preparing TSE(transmissible spongiform encephalopathy) sensitive cells is provided to develop novel drugs. CONSTITUTION: A method for preparing TSE-sensitive cells comprises: a step of isolating normal prion protein gene from the brain of an animal which infected by TSE and cloning; a step of inserting the gene to a lentivirus transfer vector to prepare infectious recombinant lentivirus; and a step of inoculating the infectious recombinant lentivirus to non-animal mammal cell line to prepare transformed cells which express a normal prion protein.

Abstract translation: 目的：提供一种制备TSE（传染性海绵状脑病）敏感细胞的方法开发新药。 构成：用于制备TSE敏感细胞的方法包括：从TSE感染的动物的脑中分离正常的朊病毒蛋白基因和克隆的步骤; 将该基因插入慢病毒转移载体以制备感染性重组慢病毒的步骤; 以及将感染性重组慢病毒接种到非动物哺乳动物细胞系以制备表达正常朊病毒蛋白的转化细胞的步骤。