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公开(公告)号：KR101144994B1
公开(公告)日：2012-06-27
申请号：KR1020080041975
申请日：2008-05-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/002 , A61K39/00
Abstract: PURPOSE: A vaccine of neosporosis and a method for producing neosporosis are provided to induce infection of low level and prevent neosporosis. CONSTITUTION: A vaccine for bovine neosporosis contains Neospora caninum antigen. The vaccine further comprises Polygen^TM, IMS1313^TM, or PBS^TM as an adjuvant. A method for producing the vaccine comprises: a step of culturing Neospora protozoa in a cell; a step of collecting cultured Neospora protozoa; and a step of treating the Neospora protozoa with antigen.
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公开(公告)号：KR1020090088169A
公开(公告)日：2009-08-19
申请号：KR1020080013557
申请日：2008-02-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , (주)래피젠
IPC: G01N33/50 , G01N30/02 , G01N33/68 , G01N33/543
Abstract: A diagnosis kit of neosporosis using immunochromatography is provided to simply and economically diagnose neosporosis without expensive equipment. A diagnosis kit using immunochromatography comprises a neospora canium antigen which is adhered on a film. The antigen is Neospora caninum Kr2 strain. The film is a nitrocellulose film. The diagnosis further comprises anti-bovine immunoglobulin G on the film.
Abstract translation: 提供使用免疫色谱法的新孢子虫病诊断试剂盒，用于简单且经济地诊断新孢子虫病，无需昂贵的设备。 使用免疫色谱法的诊断试剂盒包括粘附在膜上的新孢子虫抗原。 抗原是新孢子虫Kr2株。 该膜是硝酸纤维素膜。 该诊断还包括抗牛免疫球蛋白G。
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Patent Agency Ranking
公开(公告)号：KR1020000021058A
公开(公告)日：2000-04-15
申请号：KR1019980039989
申请日：1998-09-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N1/20
Abstract: PURPOSE: The development of a diagnostic kit of Neosporosis, which is a major cause of abortion in cattle, rapidly and accurately is provided which promotes diagnostic efficiency. CONSTITUTION: The development of a diagnostic kit is provided for diagnosing Neosporosis which is a major cause of abortion in cattle rapidly and accurately. The kit is produced by separating bovine Neospora protozoal strains from a newborn baby' brain of dairy kettle, which is incapable of standing up immediately after a birth, and culturing in large quantities by cell culturing and attaching to a Teflon-coated slide.
Abstract translation: 目的：提供快速准确地开发牛血清流产的主要原因的新孢子虫病诊断试剂盒，从而提高诊断效率。 规定：开发诊断试剂盒用于诊断新孢子虫病，这是导致牛流产的主要原因。 该试剂盒通过从新生婴儿的乳壶脑中分离牛新胞子虫原生动物菌株而产生，其不能在出生后立即站立，并通过细胞培养大量培养并附着在特氟隆涂覆的载玻片上。
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公开(公告)号：KR1020090116190A
公开(公告)日：2009-11-11
申请号：KR1020080041975
申请日：2008-05-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/002 , A61K39/00
CPC classification number: A61K39/002 , A61K2039/552 , Y10S424/823
Abstract: PURPOSE: A vaccine of neosporosis and a method for producing neosporosis are provided to induce infection of low level and prevent neosporosis. CONSTITUTION: A vaccine for bovine neosporosis contains Neospora caninum antigen. The vaccine further comprises Polygen^TM, IMS1313^TM, or PBS^TM as an adjuvant. A method for producing the vaccine comprises: a step of culturing Neospora protozoa in a cell; a step of collecting cultured Neospora protozoa; and a step of treating the Neospora protozoa with antigen.
Abstract translation: 目的：提供新孢子虫病疫苗和生产新孢子虫病的方法，以诱导低水平感染并预防新孢子虫病。 构成：牛新孢子虫病疫苗含有新孢子虫犬抗原。 疫苗还包含Polygen TM，IMS1313？TM或作为佐剂的PBS ^ TM。 疫苗的制造方法包括：在细胞中培养新孢子虫原生动物的步骤; 收集培养的新孢子虫原生动物的一个步骤; 以及用抗原处理新孢子虫原生动物的步骤。
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公开(公告)号：KR100995291B1
公开(公告)日：2010-11-19
申请号：KR1020080013557
申请日：2008-02-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , (주)래피젠
IPC: G01N33/50 , G01N30/02 , G01N33/68 , G01N33/543
Abstract: 본 발명은 면역크로마토그래피를 이용한 네오스포라병의 진단 키트에 관한 것이다. 보다 구체적으로는, 세포 배양된 국내 분리주 네오스포라 원충의 항원을 이용하여 면역크로마토그래피법을 이용한 혈청학적 진단법에 관한 것으로, 본 발명에 따르면, 막에 항원을 결합시킨 후 가검 혈청과의 반응여부를 육안적으로 관찰하여 판단하기 때문에, 본 발명에 의해 고가의 장비를 필요로 하지 않는 간편하고 경제적 진단법을 제공할 수 있다.
소, 유·사산, 네오스포라병, 면역크로마토그래피, 진단방법-
6.发明授权
公开(公告)号：KR100809952B1
公开(公告)日：2008-03-06
申请号：KR1020060044347
申请日：2006-05-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 곰팡이독소 디옥시니발레놀에 대한 특이단백질 햅토글로빈을 이용한 디옥시니발레놀 중독증 및 노출 진단방법 및 진단키트에 관한 것으로, 더욱 상세하게는 단백질칩 기술을 이용하여 곰팡이독소 중 구토독소인 디옥시니발레놀(Deoxynivalenol)에 대한 생체특이발현 단백질을 확인하고 이를 이용하여 디옥시니발레놀 노출 및 중독증을 조기에 진단하는 방법에 관한 것이다.
본 발명에서는 곰팡이독소 DON에 중독되었을 경우 혈장 중에 특이적으로 햅토글로빈이 발현되는 것을 세계최초로 규명하였는바, 이를 이용하면 디옥시니발레놀의 생체노출정도 및 중독증을 진단하는데 용이하게 이용될 수 있다.
곰팡이독소, 특이발현단백질, 단백질칩, SELDI - TOF/MS, Haptoglobin-
7.发明公开
公开(公告)号：KR1020070111231A
公开(公告)日：2007-11-21
申请号：KR1020060044347
申请日：2006-05-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A method and kit for diagnosis of toxicity and exposure for deoxynivalenol are provided to improve detection convenience and time of deoxynivalenol by detecting increase of a haptoglobin level in blood plasma. A method for diagnosis of toxicity and exposure for deoxynivalenol comprises the steps of: coating an ELISA(enzyme-linked immunosorbent assay) plate with an anti-haptoglobin antibody; adding diluted blood plasma into the plate and reacting them for a certain period; reacting the plate with a secondary antibody to determine presence of haptoglobin having the amino acid sequence of SEQ ID NO:1. A kit for diagnosis of toxicity and exposure for deoxynivalenol comprises an antibody specific to haptoglobin or its immunogenic fragment, fixed in a reaction region of a strip.
Abstract translation: 提供了用于诊断脱氧雪腐镰刀菌烯醇的毒性和暴露的方法和试剂盒，通过检测血浆中触珠蛋白水平的增加来提高脱氧雪腐镰刀菌烯醇的检测方便性和时间。 用于诊断脱氧雪腐镰刀菌烯醇的毒性和暴露的方法包括以下步骤：用抗 - 触珠蛋白抗体包被ELISA（酶联免疫吸附测定）板; 将稀释的血浆加入板中并使其反应一定时间; 使该板与第二抗体反应以确定具有SEQ ID NO：1的氨基酸序列的触珠蛋白的存在。 用于诊断毒性的试剂盒和脱氧雪腐镰刀菌烯醇的暴露包括固定在条带的反应区域中对触珠蛋白或其免疫原性片段特异的抗体。
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