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公开(公告)号:KR100496026B1
公开(公告)日:2005-06-16
申请号:KR1020020079315
申请日:2002-12-12
Applicant: 대한민국(농촌진흥청장)
IPC: A01H4/00
Abstract: 본 발명은 철분강화 감자에 관한 것으로서, ferritin 유전자를 감자에서 증강시켜 같은 환경조건하에서 철분 함량을 많이 보유하는 감자 보급종 품종 또는 유종모본을 개발함으로서, 향후 음식물의 철분 결핍문제를 해결할 수 있도록 하기위한 것이다.
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公开(公告)号:KR1020040051389A
公开(公告)日:2004-06-18
申请号:KR1020020079315
申请日:2002-12-12
Applicant: 대한민국(농촌진흥청장)
IPC: A01H4/00
CPC classification number: C12N15/8243 , C12N15/8205 , C12N15/8216
Abstract: PURPOSE: Iron fortified potato is provided, therein increasing the expression of the ferritin gene within potato, and resolving the iron deficient problems in food. CONSTITUTION: The method for producing the iron fortified potato comprises the steps of: connecting a ferritin gene of soybean to 35S CaMV vector; inserting PinII terminator into the 35S CaMV vector; transferring the 35S CaMV vector into pCAMBIA3300 binary vector; transforming potato with the pCAMBIA3300 binary vector containing the ferritin gene of soybean by co-culturing Agrobacterium tumefaciens LBA4404 with potato in MSO medium for 48 hours; inducing callus of infected potato in a callus inducing medium; and forming roots of infected potato in a root inducing medium.
Abstract translation: 目的:提供铁强化马铃薯,增加马铃薯内铁蛋白基因的表达,解决食物中缺铁问题。 构成:铁强化马铃薯的生产方法包括:将大豆铁蛋白基因连接到35S CaMV载体上; 将PinII终止子插入35S CaMV载体; 将35S CaMV载体转移到pCAMBIA3300二元载体中; 通过与MSO培养基中的马铃薯共培养根癌土壤杆菌LBA4404 48小时,通过含有大豆铁蛋白基因的pCAMBIA3300二元载体转化马铃薯; 在愈伤组织诱导培养基中诱导感染马铃薯的愈伤组织; 并在根诱导培养基中形成感染马铃薯的根。
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