철분강화 감자
    2.
    发明公开
    철분강화 감자 有权
    铁ED ED POTATO

    公开(公告)号:KR1020040051389A

    公开(公告)日:2004-06-18

    申请号:KR1020020079315

    申请日:2002-12-12

    CPC classification number: C12N15/8243 C12N15/8205 C12N15/8216

    Abstract: PURPOSE: Iron fortified potato is provided, therein increasing the expression of the ferritin gene within potato, and resolving the iron deficient problems in food. CONSTITUTION: The method for producing the iron fortified potato comprises the steps of: connecting a ferritin gene of soybean to 35S CaMV vector; inserting PinII terminator into the 35S CaMV vector; transferring the 35S CaMV vector into pCAMBIA3300 binary vector; transforming potato with the pCAMBIA3300 binary vector containing the ferritin gene of soybean by co-culturing Agrobacterium tumefaciens LBA4404 with potato in MSO medium for 48 hours; inducing callus of infected potato in a callus inducing medium; and forming roots of infected potato in a root inducing medium.

    Abstract translation: 目的:提供铁强化马铃薯,增加马铃薯内铁蛋白基因的表达,解决食物中缺铁问题。 构成:铁强化马铃薯的生产方法包括:将大豆铁蛋白基因连接到35S CaMV载体上; 将PinII终止子插入35S CaMV载体; 将35S CaMV载体转移到pCAMBIA3300二元载体中; 通过与MSO培养基中的马铃薯共培养根癌土壤杆菌LBA4404 48小时,通过含有大豆铁蛋白基因的pCAMBIA3300二元载体转化马铃薯; 在愈伤组织诱导培养基中诱导感染马铃薯的愈伤组织; 并在根诱导培养基中形成感染马铃薯的根。

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