公开(公告)号：KR100375673B1

公开(公告)日：2003-03-15

申请号：KR1020000004429

申请日：2000-01-29

Applicant: 대한민국(농촌진흥청장)

Inventor： 이현정 ,  이상철 ,  김진욱 ,  신기준 ,  최윤재 ,  강승하 ,  복진덕

IPC: C12N15/63

Abstract: PURPOSE: Provided is a recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase derived from gram-positive bacteria, Enterobactor sp.4. And transformant Pichia pastoris pGAP-phyA is also provided, which can secret myo-inositol hexaphosphate phosphohydrolase continuously, and is useful as a feed additive. CONSTITUTION: A recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase is prepared by the steps of: (a) separating myo-inositol hexaphosphate phosphohydrolase chromosome derived from Enterobactor sp.4; (b) cloning and sequencing the base sequence of the gene coding myo-inositol hexaphosphate phosphohydrolase originated from gram-positive bacteria, Enterobactor sp.4; (c) constructing an expression vector including a gene fragment for coding myo-inositol hexaphosphate phosphohydrolase; (d) transforming Pichia pastoris GS 115 yeast strain with the vector; and (e) selecting a transformant.

Abstract translation: 目的：提供一种重组载体pGAP-phyA，其包括编码肌酸六磷酸磷酸水解酶的肌醇六磷酸酶基因，所述肌醇六磷酸磷酸水解酶来源于革兰氏阳性细菌Enterobactor sp.4。 还提供了转化体巴斯德毕赤酵母pGAP-phyA，其可以连续分泌肌醇六磷酸磷酸水解酶，并且可用作饲料添加剂。 构成：通过以下步骤制备包含编码肌醇六磷酸磷酸水解酶的植酸酶基因的重组载体pGAP-phyA：（a）分离源自Enterobactor sp.4的肌醇六磷酸磷酸水解酶染色体; （b）克隆和测序编码源自革兰氏阳性细菌肌醇六磷酸磷酸水解酶的基因的碱基序列，Enterobactor sp.4; （c）构建包含编码肌肉六磷酸磷酸水解酶的基因片段的表达载体; （d）用载体转化巴斯德毕赤酵母GS115酵母菌株; 和（e）选择转化体。

公开(公告)号：KR1020010076962A

公开(公告)日：2001-08-17

申请号：KR1020000004429

申请日：2000-01-29

Applicant: 대한민국(농촌진흥청장)

Inventor： 이현정 ,  이상철 ,  김진욱 ,  신기준 ,  최윤재 ,  강승하 ,  복진덕

IPC: C12N15/63

CPC classification number: C12N15/63 ,  A23K10/16 ,  A61K35/741

Abstract: PURPOSE: Provided is a recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase derived from gram-positive bacteria, Enterobactor sp.4. And transformant Pichia pastoris pGAP-phyA is also provided, which can secret myo-inositol hexaphosphate phosphohydrolase continuously, and is useful as a feed additive. CONSTITUTION: A recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase is prepared by the steps of: (a) separating myo-inositol hexaphosphate phosphohydrolase chromosome derived from Enterobactor sp.4; (b) cloning and sequencing the base sequence of the gene coding myo-inositol hexaphosphate phosphohydrolase originated from gram-positive bacteria, Enterobactor sp.4; (c) constructing an expression vector including a gene fragment for coding myo-inositol hexaphosphate phosphohydrolase; (d) transforming Pichia pastoris GS 115 yeast strain with the vector; and (e) selecting a transformant.

Abstract translation: 目的：提供一种重组载体pGAP-phyA，其中包括植酸酶基因，其编码来自革兰氏阳性菌的Entero反应器sp.4的肌醇六磷酸磷酸水解酶。 还提供了转化体巴斯德毕赤酵母pGAP-phyA，其可以连续分泌肌醇六磷酸磷酸水解酶，并且可用作饲料添加剂。 构成：通过以下步骤制备包含编码肌醇六磷酸磷酸水解酶的肌醇六磷酸酶基因的重组载体pGAP-phyA：（a）分离源自Enterobactor sp.4的肌醇六磷酸磷酸水解酶染色体; （b）克隆和测序来源于革兰氏阳性菌的Enterobactor sp.4的编码肌醇六磷酸磷酸水解酶的基因的碱基序列; （c）构建包含编码肌醇六磷酸磷酸水解酶的基因片段的表达载体; （d）用载体转化巴斯德毕赤酵母GS 115酵母菌株; 和（e）选择转化体。