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公开(公告)号：KR101444297B1
公开(公告)日：2014-10-15
申请号：KR1020120001427
申请日：2012-01-05
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/113 , A01K67/027
Abstract: 본 발명은 CYP4F16 유전자가 넉다운된 형질전환마우스 및 그 제조방법에 관한 것이다. 보다 상세하게는, 수면병에 대한 저항성을 나타내는 CYP4F16유전자가 넉다운된 형질전환마우스 및 그 제조방법에 관한 것이다. 본 발명의 형질전환 마우스를 이용하면, 트리파노솜의 체내 침입후 발생되는 면역관련 유전자 중 수면병의 발생생원인 규명하고, 이를 치료할 수 있는 신약을 개발하는 데 이용될 수 있다.
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公开(公告)号：KR1020130080572A
公开(公告)日：2013-07-15
申请号：KR1020120001427
申请日：2012-01-05
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/113 , A01K67/027
Abstract: PURPOSE: A transgenic mouse in which CYP4F16 gene is knocked-down and a manufacturing method thereof are provided to inquire a cause of occurrence of a sleeping disease among immune-related genes generated after infestation of trypanosome and to develop new medicine for curing the diseases. CONSTITUTION: A siRNA expression recombinant vector for mouse CYP4F16 knocked-down is identified by a promoter and SEQ ID No. 1 that is operably connected to the promoter. The promoter is a U6 promoter. The recombinant vector is introduced to a transgenic mouse. A manufacturing method of a transgenic mouse in which CYP4F16 gene expression is knocked-down comprises the following steps of: (a) manufacturing a siRNA expression cassette for expressing CYP4F16 that is represented by a promoter and SEQ ID No. 1 that is operably connected to the promoter; (b) introducing the recombinant fused gene to a fertilized egg of a mouse; and (c) producing breeders by implanting the fertilized egg into a surrogate mother.
Abstract translation: 目的：提供CYP4F16基因被敲除的转基因小鼠及其制造方法，用于询问在锥虫感染后产生的免疫相关基因中发生睡眠疾病的原因，并开发用于治疗疾病的新药。 构成：用于小鼠CYP4F16敲除的siRNA表达重组载体通过与启动子可操作连接的启动子和SEQ ID No.1来鉴定。 启动子是U6启动子。 将重组载体导入转基因小鼠。 其中CYP4F16基因表达被敲除的转基因小鼠的制造方法包括以下步骤：（a）制备用于表达CYP4F16的siRNA表达盒，所述siRNA表达盒由启动子和SEQ ID No.1表示，所述启动子和SEQ ID No.1可操作地连接到 启动子; （b）将重组融合基因导入小鼠受精卵; 和（c）通过将受精卵植入替代母亲来培育育种者。
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